1. Identification of ICIS-1, a new protein involved in cilia stability
- Author
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Frédéric Tournier, Seltzer, Gallinger J, Sandrine Middendorp, Skowron-Zwarg M, Ponsard C, Fisch C, Dupuis-Williams P, Eric Perret, Caruso N, Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Université Paris Diderot - Paris 7 (UPD7), Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Unité de Biologie Moléculaire du Gène, Sanofi Aventis Recherche, Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Développement et évolution (DE), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Département Interactions Physique, Chimie et Vivant (DIPCV-IPHC), and Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Axoneme ,Protozoan Proteins ,Sequence Homology ,MESH: Amino Acid Sequence ,MESH: Base Sequence ,MESH: Transforming Growth Factor beta1 ,RNA interference ,Gene expression ,Basal body ,Tissue Distribution ,MESH: Animals ,MESH: Proteins ,MESH: Sequence Homology ,Promoter Regions, Genetic ,MESH: Phylogeny ,MESH: Protozoan Proteins ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Cells, Cultured ,Phylogeny ,0303 health sciences ,Differential display ,Cilium ,030305 genetics & heredity ,Cell Differentiation ,Fluid transport ,Cell biology ,MESH: Promoter Regions (Genetics) ,MESH: Nasal Mucosa ,RNA Interference ,MESH: Cells, Cultured ,MESH: Cell Differentiation ,MESH: Paramecium tetraurelia ,Molecular Sequence Data ,MESH: RNA Interference ,Biology ,Transforming Growth Factor beta1 ,03 medical and health sciences ,MESH: Cilia ,Ciliogenesis ,Animals ,Humans ,Amino Acid Sequence ,Cilia ,MESH: Tissue Distribution ,030304 developmental biology ,MESH: Humans ,MESH: Molecular Sequence Data ,Base Sequence ,Proteins ,Nasal Mucosa ,Paramecium tetraurelia - Abstract
International audience; Cilia are specialized organelles that exert critical functions in numerous organisms, including that of cell motility, fluid transport and protozoan locomotion. Ciliary architecture and function strictly depend on basal body formation, migration and axoneme elongation. Numerous ultrastructural studies have been undertaken in different species to elucidate the process of ciliogenesis. Recent analyses have led to identification of genes specifically expressed in ciliated organisms, but most proteins involved in ciliogenesis remain uncharacterized. Using human nasal epithelial cells capable of ciliary differentiation in vitro, differential display was carried out to identify new proteins associated with ciliogenesis. We isolated a new gene, ICIS-1 (Involved in CIlia Stability-1), upregulated during mucociliary differentiation. This gene is localized within the TGF-beta1 promoter and is ubiquitously expressed in human tissues. Functional analyses of gene expression inhibition by RNA interference in Paramecium tetraurelia indicated that the ICIS-1 homologue interfered with cilia stability or formation. These findings demonstrate that ICIS-1 is a new protein associated with ciliated cells and potentially related to cilia stability.
- Published
- 2007
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