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Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.

The aim of this paper is to examine the effect of different sound stress duration on adrenal gland of broiler chickens of different age. The experiments were conducted on 90 HYBRO broiler chickens, divided into nine groups (each group consisting of 10 birds) by different age and lenght of sound stress duration. The chickens were exposed to sound stress in sound attenuated building using a fire alarm bell (95 dB) for stress sessions lasted 120 min every day. The histological structure of adrenal gland was analyzed by light microscopy. The results indicate that adrenal gland is sensitive on this kind of stress and sound as stressor could introduce organism in stress reaction. Adrenal glands of broilers exposed to sound stress characterized by marked hyperemia, hypertrophy of interrenal cells, smaller affinity for staining of chromaffin cells compared with control groups. According to our results, degree of histological changes of adrenal gland under the influence of sound stress depends on the length of exposure and age of chickens. Cilj ovog rada je da ispita uticaj različite dužine trajanja zvučnog stresa na histološku građu nadbubrežnih žlezda brojlerskih pilića u različitim uzrastima. Ogled je izveden na 90 HYBRO brojlerskih pilića, podeljenih u 9 grupa u skladu sa uzrastom i dužinom izlaganja stresu. Pilići su zvučnom stresu bili izlagani u zvučno izolovanim objektima uz korišćenje požarnog alarmnog zvona (95dB) u trajanju od 120 min svakog dana. Histološka građa nadbubrežnih žlezda je analizirana svetlosnim mikroskopom. Rezultati ukazuju da je nadbubrežna žlezda osetljiva na ovu vrstu stresa i da zvuk, kao stresor, može da uvede organizam u stresnu reakciju. Nadbubrežne žlezde pilića izlaganih zvučnom stresu karakteriše značajna hiperemija, hipertrofija interrenalnih ćelija, manji afinitet prema bojenju hromafinih ćelija u odnosu na kontrolnu grupu. U skladu sa ovim rezultatima, stepen histoloških promena nadbubrežne žlezde pod uticajem zvučnog stresa zavisi i od dužine izlaganja i uzrasta pilića.

42nd European-Society-for-Medical-Oncology Congress (ESMO), Sep 08-12, 2017, Madrid, Spain

It is proved that the protein expression of IGF-1 is detected in several tissues including liver, brain, eye, lung, pancreas and muscle. This growth factor exerts autocrine and paracrine effect during embryonic and postnatal development and is involved in muscle development. The aim of this study was to determine the level of protein expression of IGF-1 in breast muscle during embryonic and postnatal development of broiler chickens. For the experiment were used fertilized eggs and broiler chickens of heavy hybrid "Ross 308", originating from parent flock at the age of 54 weeks. Samples of breast muscle were taken from embryos during the 15th and 19th day of embryonic development and 3rd and 21st day of postnatal development from 5 birds in each age category. From all of the embryos and chickens the right side of the brest muscles were individually sampled. The muscle tissue was macerated and then homogenized in buffer with protease inhibitors. After that immunoprecipitation, electrophoresis and transfer the Western blot analysis were performed on all samples. For visualization of proteins the membranes were incubated in chemiluminescent substrate. The results indicate statistically significant increase in protein expression of IGF-1 in breast muscle on the 19th day of embryonic development and 3rd of postnatal development in compared to 15th day of embryonic development (p

Embryonic stage of development, in conditions of intensive broiler production, is a period in which the dynamics of postnatal development and the productive performance can be affected significantly. Green monochromatic light and thermal conditioning are the most commonly used treatments that have given satisfactory results in postnatal development. The aim of this study was to examine the effect of these treatments on meat quality of broiler chickens. Our study is based on the four experimental groups (control and three different treatments). Each group was incubated in separate incubator with 80 eggs, with controlled incubation parameters (temperature, humidity, ventilation). The control group was incubated under standard conditions (37.8 ?C, without light). The first treatment consisted of thermal conditioning (raising the temperature to 39,8 ?C for 3 h on 16th, 17th and 18th day of incubation). Another treatment consisted of lighting a green monochrome light (diffused LED lighting intensity of 0.1 W/m2) from 5th until 14th day of incubation, in the intermittent mode (15 min. light, 15 min. darkness), and from 15th day of incubation under constant light regime. The third treatment was a combination of first two treatments, with the temperature and lighting changed by these regimes. Our results indicate a significantly higher average body weight, protein content in meat, and less progressive loss of moisture (drip loss) in all treated groups. The applied treatments had no effect on mortality, conversion, or the content of fat and ash in meat. The results also indicate a significant synergistic effects achieved by the simultaneous application of both treatments.