1. Plant-derived bioactive compounds produced by Streptomyces variabilis LCP18 associated with Litsea cubeba (Lour.) Pers as potential target to combat human pathogenic bacteria and human cancer cell lines
- Author
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Ngoc Tung Quach, Quang Huy Nguyen, Thi Hanh Nguyen Vu, Thi Thu Hang Le, Thi Thu Thuy Ta, Tien Dat Nguyen, Thuoc Van Doan, The Van Nguyen, Tat Thanh Dang, Xuan Canh Nguyen, Hoang Ha Chu, and Quyet Tien Phi
- Subjects
Lung Neoplasms ,Litsea ,Phytochemicals ,Biology ,Streptomyces variabilis ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Staphylococcus epidermidis ,Cell Line, Tumor ,Media Technology ,medicine ,Humans ,Biotechnology and Industrial Microbiology - Research Paper ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Plant Extracts ,Daidzein ,food and beverages ,Litsea cubeba ,Pathogenic bacteria ,Syringic acid ,Daucosterol ,biology.organism_classification ,Streptomyces ,Anti-Bacterial Agents ,chemistry ,A549 Cells ,Antibacterial activity - Abstract
To date, endophytic actinomycetes have been well-documented as great producers of novel antibiotics and important pharmaceutical leads. The present study aimed to evaluate potent bioactivities of metabolites synthesized by the strain LCP18 residing in the Vietnamese medicinal plant Litsea cubeba (Lour.) Pers towards human pathogenic bacteria and human cancer cell lines. Endophytic actinomycete strain LCP18 showed considerable inhibition against seven bacterial pathogens and three human tumor cell lines and was identified as species Streptomyces variabilis. Strain S. variabilis LCP18 was phenotypically resistant to fosfomycin, trimethoprim-sulfamethoxazole, dalacin, cefoxitin, rifampicin, and fusidic acid and harbored the two antibiotic biosynthetic genes such as PKS-II and NRPS. Further purification and structural elucidation of metabolites from the LCP18 extract revealed five plant-derived bioactive compounds including isopcrunetin, genistein, daidzein, syringic acid, and daucosterol. Among those, isoprunetin, genistein, and daidzein exhibited antibacterial activity against Salmonella typhimurium ATCC 14,028 and methicillin-resistant Staphylococcus epidermidis ATCC 35,984 with the MIC values ranging from 16 to 128 µg/ml. These plant-derived compounds also exhibited cytotoxic effects against human lung cancer cell line A549 with IC(50) values of less than 46 μM. These findings indicated that endophytic S. variabilis LCP18 can be an alternative producer of plant-derived compounds which significantly show potential applications in combating bacterial infections and inhibition against lung cancer cell lines.
- Published
- 2021