1. Identification of peptide biomarkers for authentication of Atlantic salmon and rainbow trout with untargeted and targeted proteomics approaches and quantitative detection of adulteration
- Author
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Fang Han, Shuqing Gu, Dehua Guo, Yiyin Shi, Xiaojun Deng, Yichun Cai, and Yihui Huo
- Subjects
Fish Proteins ,Proteomics ,endocrine system ,animal structures ,Proteome ,animal diseases ,Clinical Biochemistry ,Salmo salar ,Peptide ,Food Contamination ,Computational biology ,digestive system ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Limit of Detection ,Animals ,chemistry.chemical_classification ,Chromatography ,urogenital system ,010401 analytical chemistry ,Reproducibility of Results ,Cell Biology ,General Medicine ,0104 chemical sciences ,Targeted proteomics ,chemistry ,Seafood ,Close relationship ,Oncorhynchus mykiss ,Linear Models ,Biomarker (medicine) ,Rainbow trout ,Biomarkers - Abstract
Atlantic salmon is often adulterated or substituted by rainbow trout with much lower price and quality. However, it is extremely difficult to distinguish Atlantic salmon and rainbow trout due to their similar appearance and close relationship in species. In the present work, untargeted and targeted proteomics approaches were both implemented to identify species-specific peptide biomarkers of Atlantic salmon and rainbow trout. Potential peptide biomarkers were obtained through matching HRMS data with UniProt database, screened by BLAST and then verified with real samples. Five peptide biomarkers were identified each for Atlantic salmon and rainbow trout. MRM method was established for quantitative measurement of rainbow trout Adulteration in Atlantic salmon, showing high sensitivity and repeatability. The biomarker peptide GDPGPGGPQGEQGVVGPAGISGDK was used for quantification. The limit of the detection (LOD) of adulteration of rainbow trout is 0.19%, and the limit of quantitation (LOQ) is 0.62%. Furthermore, this method was successfully applied to analyze a number of Atlantic salmon and Rainbow trout samples from different regions and different batches, as well as commercially available processed products.
- Published
- 2020