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Your search keyword '"MESH: Cell-Free Nucleic Acids"' showing total 2 results
Start Over Descriptor "MESH: Cell-Free Nucleic Acids" Topic 030220 oncology & carcinogenesis
2 results on '"MESH: Cell-Free Nucleic Acids"'

1. Comparison of five cell-free DNA isolation methods to detect the EGFR T790M mutation in plasma samples of patients with lung cancer

Author

Romain Senal, Marion Larrieux, Jérôme Solassol, Julie A. Vendrell, Alain Mangé, David Grand, Eleftherios P. Diamandis, Xavier Quantin, Patricia Audran, Service de Biopathologie [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Laboratoire de génétique des maladies rares. Pathologie moleculaire, etudes fonctionnelles et banque de données génétiques (LGMR), Université Montpellier 1 (UM1)-IFR3, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), CRLCC Val d'Aurelle - Paul Lamarque, Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Mount Sinai Hospital [Toronto, Canada] (MSH), University of Toronto, and University Health Network

Subjects
0301 basic medicine, medicine.medical_treatment, Clinical Biochemistry, MESH: Reagent Kits, Diagnostic, EGFR T790M, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: ErbB Receptors, Targeted therapy, droplet digital PCR, 03 medical and health sciences, 0302 clinical medicine, medicine, Digital polymerase chain reaction, Lung cancer, ComputingMilieux_MISCELLANEOUS, circulating tumor DNA, MESH: Humans, Plasma samples, business.industry, MESH: Real-Time Polymerase Chain Reaction, Biochemistry (medical), General Medicine, medicine.disease, Isolation (microbiology), targeted therapy, 3. Good health, MESH: Lung Neoplasms, lung cancer, 030104 developmental biology, Cell-free fetal DNA, MESH: Gene Deletion, 030220 oncology & carcinogenesis, Mutation (genetic algorithm), Cancer research, MESH: Cell-Free Nucleic Acids, EGFR mutation, business, MESH: Exons, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2018
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2. Droplet digital PCR detects high rate of TP53 R249S mutants in cell-free DNA of middle African patients with hepatocellular carcinoma

Author

Aubin Béré, Pascal Pineau, Paul Jean Adrien Atangana, Dominique Noah Noah, Claudine Bekondi, Narcisse-Patrice Komas, Marie Amougou Atsama, Agnès Marchio, Richard Njouom, Serge-Magloire Camengo-Police, Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Bangui, Hôpital Central de Yaoundé, Hôpital de l'Amitié [Bangui], This work was supported by Institut Pasteur International Network (Action Concertée Inter-Pasteurienne No17-2010, and Grant Dedonder), by the direction of Research Applications and Relations with the Industry (VALO Express), and by the French Ligue Nationale contre le Cancer (Equipe labélisée d’Anne Dejean)., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and MARCHIO, Agnes

Subjects
0301 basic medicine, Male, Aflatoxin B1, MESH: Mutation Rate, Hepatocellular carcinoma, Gene Expression, medicine.disease_cause, Gastroenterology, Polymerase Chain Reaction, Cell-free DNA, 0302 clinical medicine, MESH: Aged, 80 and over, Mutation Rate, MESH: Liver Neoplasms, Digital polymerase chain reaction, Cameroon, MESH: Tumor Suppressor Protein p53, MESH: Carcinoma, Hepatocellular, Aged, 80 and over, MESH: Aged, education.field_of_study, Hematology, MESH: Middle Aged, Liver Neoplasms, MESH: Aflatoxin B1, General Medicine, Middle Aged, Viral Load, MESH: Case-Control Studies, 3. Good health, Central African Republic, MESH: Hepatitis B virus, Cell-free fetal DNA, Droplet digital PCR, MESH: Central African Republic, 030220 oncology & carcinogenesis, Female, alpha-Fetoproteins, Liver cancer, MESH: Viral Load, Cell-Free Nucleic Acids, Adult, medicine.medical_specialty, Hepatitis B virus, Carcinoma, Hepatocellular, MESH: Gene Expression, Adolescent, MESH: Hepatitis B, Chronic, Population, [SDV.CAN]Life Sciences [q-bio]/Cancer, Malignancy, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Hepatitis B, Chronic, [SDV.CAN] Life Sciences [q-bio]/Cancer, Internal medicine, medicine, Humans, education, Aged, MESH: Adolescent, Hepatitis B Surface Antigens, MESH: Humans, business.industry, MESH: Adult, MESH: Polymerase Chain Reaction, MESH: Cameroon, medicine.disease, MESH: Male, MESH: DNA, Viral, MESH: Hepatitis B Surface Antigens, 030104 developmental biology, TP53 mutants, Case-Control Studies, DNA, Viral, MESH: Cell-Free Nucleic Acids, MESH: alpha-Fetoproteins, Tumor Suppressor Protein p53, business, MESH: Female
Abstract

International audience; Hepatocellular carcinoma (HCC) is still a major killing malignancy in sub-Saharan Africa. Lifelong intoxication with aflatoxin B1 is considered as one of the primary causes of this situation. The role of aflatoxin in HCC from a given population is commonly estimated through the prevalence of R249S mutation of TP53, a hallmark for previous exposure to the mycotoxin. However, the role of AFB1 is barely known in large part of Africa. We conducted a survey on circulating cell-free DNA from 149 patients with HCC and 213 control subjects with and without liver diseases from Cameroon and Central African Republic using droplet digital PCR technique. We observed a mutation prevalence of 24.8% (n = 37/149) in patients with tumor and 5.6% (n = 12/213) in controls (P = 2.2E-07). Patients with mutations usually displayed significantly increased circulating alpha-fetoprotein (AFP) values, high hepatitis B virus (HBV) DNA loads as well as worsened values of blood cells count. Interestingly, the fraction of droplets positive for R249S was significantly larger in patients with liver cancer (15.3 ± 3.7%) than in controls (0.5 ± 0.3%, P = 7.1E-04). Our survey indicates that AFB1 is instrumental for HCC development in Middle Africa and that droplet digital PCR might be used in the region both to diagnose HCC and to conduct public health surveys on populations at risk of chronic aflatoxin intoxication.

Published
2018
Full Text
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