1. Bile duct reconstruction using scaffold-free tubular constructs created by Bio-3D printer
- Author
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Shun Yamaguchi, Susumu Eguchi, Tota Kugiyama, Akihiko Soyama, Takayuki Miyoshi, Akihide Kamiya, Mitsuhisa Takatsuki, Masaaki Hidaka, Takanobu Hara, Koichi Nakayama, Anna Nakamura, Takashi Hamada, and Yusuke Sakai
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Scaffold ,Medicine (General) ,Angiogenesis ,EDTA, trypsin-ethylenediaminetetraacetic acid ,medicine.medical_treatment ,Biomedical Engineering ,PBS, phosphate-buffered saline ,Intrahepatic bile ducts ,Lumen (anatomy) ,Cr, creatinine ,Artificial bile duct ,AST, aspartate aminotransferase ,Biomaterials ,DMEM, Dulbecco's Modified Eagle's Medium ,03 medical and health sciences ,0302 clinical medicine ,R5-920 ,Bio-3D printer ,FBS, fetal bovine serum ,ALT, alanine aminotransferase ,medicine ,IBDI, iatrogenic bile duct injury ,T-Bil, total bilirubin ,Fibroblast ,Scaffold-free tubular construct ,γ-GTP, γ-glutamyl transpeptidase ,QH573-671 ,ALP, alkaline phosphatase ,LDLT, living donor liver transplantation ,business.industry ,Bile duct ,Stent ,QOL, quality of life ,030104 developmental biology ,medicine.anatomical_structure ,Immunohistochemistry ,Original Article ,Reconstruction ,business ,Cytology ,030217 neurology & neurosurgery ,Developmental Biology ,KCL, potassium chloride - Abstract
Introduction Biliary strictures after bile duct injury or duct-to-duct biliary reconstruction are serious complications that markedly reduce patients’ quality of life because their treatment involves periodic stent replacements. This study aimed to create a scaffold-free tubular construct as an interposition graft to treat biliary complications. Methods Scaffold-free tubular constructs of allogeneic pig fibroblasts, that is, fibroblast tubes, were created using a Bio-3D Printer and implanted into pigs as interposition grafts for duct-to-duct biliary reconstruction. Results Although the fibroblast tube was weaker than the native bile duct, it was sufficiently strong to enable suturing. The pigs' serum hepatobiliary enzyme levels remained stable during the experimental period. Micro-computed tomography showed no biliary strictures, no biliary leakages, and no intrahepatic bile duct dilations. The tubular structure was retained in all resected specimens, and the fibroblasts persisted at the graft sites. Immunohistochemical analyses revealed angiogenesis in the fibroblast tube and absence of extensions of the biliary epithelium into the fibroblast tube's lumen. Conclusions This study's findings demonstrated successful reconstruction of the extrahepatic bile duct with a scaffold-free tubular construct created from pig fibroblasts using a novel Bio-3D Printer. This construct could provide a novel regenerative treatment for patients with hepatobiliary diseases.
- Published
- 2021