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Start Over Author "Sandy Peltier" Topic 0302 clinical medicine
3 results on '"Sandy Peltier"'

1. Failed Disruption of Tick Feeding, Viability, and Molting after Immunization of Mice and Sheep with Recombinant Ixodes ricinus Salivary Proteins IrSPI and IrLip1

Author

Martine Cote, Ladislav Šimo, Sarah Bonnet, Sandy Peltier, Jennifer Maye, Consuelo Almazán, Nicolas Versillé, Jennifer Richardson, Sabine Rakotobe, Lisa Fourniol, Jeremie Bornères, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Air Liquide Healthcare, Air Liquide [Siège Social], Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ERGANEO (ex SATTIdF Innov), Animal Health Department of INRAE, Ceva Santé Animale, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0301 basic medicine, Ixodes ricinus, 030231 tropical medicine, Immunology, lcsh:Medicine, salivary proteins, Tick, Microbiology, ticks, 03 medical and health sciences, 0302 clinical medicine, Immune system, Antigen, Drug Discovery, parasitic diseases, Pharmacology (medical), Pathogen, Pharmacology, biology, lcsh:R, Ricinus, fungi, biology.organism_classification, bacterial infections and mycoses, 3. Good health, Vaccination, 030104 developmental biology, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Immunization, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, anti-tick vaccination
Abstract

To identify potential vaccine candidates against Ixodes ricinus and tick-borne pathogen transmission, we have previously sequenced the salivary gland transcriptomes of female ticks infected or not with Bartonella henselae. The hypothesized potential of both IrSPI (I. ricinus serine protease inhibitor) and IrLip1 (I. ricinus lipocalin 1) as protective antigens decreasing tick feeding and/or the transmission of tick-borne pathogens was based on their presumed involvement in dampening the host immune response to tick feeding. Vaccine endpoints included tick larval and nymphal mortality, feeding, and molting in mice and sheep. Whether the antigens were administered individually or in combination, the vaccination of mice or sheep elicited a potent antigen-specific antibody response. However, and contrary to our expectations, vaccination failed to afford protection against the infestation of mice and sheep by I. ricinus nymphs and larvae, respectively. Rather, vaccination with IrSPI and IrLip1 appeared to enhance tick engorgement and molting and decrease tick mortality. To the best of our knowledge, these observations represent the first report of induction of vaccine-mediated enhancement in relation to anti-tick vaccination.

Published
2020
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2. Plasma cell-directed therapies in monoclonal gammopathy-associated scleromyxedema

Author

Sébastien Barbarot, O. Carpentier, François Lifermann, Bernard Cribier, Jean-Luc Schmutz, Amélie Osio, Marie Jachiet, Cristina Bulai Livideanu, Marie Beylot-Barry, Martine Bagot, Fabien Le Bras, Jean-David Bouaziz, Marie Le Moigne, Amandine Servy, Emilie Sbidian, Alexis Talbot, Laurence Michel, Pierre Aucouturier, Nicolas Limal, Camille Francès, Marie Tauber, Philippe Humbert, Vincent Descamps, Sébastien Debarbieux, Alain Dupuy, Ruba Y. Taha, Emilie Baubion, Arsène Mekinian, Michel Rybojad, Adèle de Masson, Maxime Battistella, Charles Zarnitsky, T. Mahévas, Philippe Modiano, Michel D'Incan, Olivier Fain, Bruno Sassolas, Claire de Moreuil, Fanny Brault, Bertrand Arnulf, Sandy Peltier, D. Thomas-Beaulieu, Romain Prud'homme, Thierry Passeron, Olivier Hermine, Dan Lipsker, Sorbonne Université (SU), Hôpital Saint-Louis, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, CHU Henri Mondor, Centre Hospitalier Universitaire de Strasbourg (CHU de Strasbourg ), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Clermont-Ferrand, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), CHU Bordeaux [Bordeaux], Centre Hospitalier Universitaire de Nice (CHU Nice), National Center for Cancer Care and Research (NCCCR - DOHA - QATAR), Centre Hospitalier Universitaire [Rennes], Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire de Lyon, Centre Hospitalier de Roubaix, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), CHI Poissy-Saint-Germain, Hôpital Saint-Vincent de Paul, Centre Hospitalier, Centre Hospitalier de Dax, Centre Hospitalier Universitaire (CHU), Centre hospitalier universitaire de Nantes (CHU Nantes), CHU Toulouse [Toulouse], CHU Limoges, Université de Toulouse (UT), CHU Henri Mondor [Créteil], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)

Subjects
Adult, Male, 0301 basic medicine, medicine.medical_specialty, medicine.medical_treatment, Plasma Cells, Immunology, Paraproteinemias, Antineoplastic Agents, Plasma cell, Biochemistry, Gastroenterology, Dexamethasone, Bortezomib, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Scleromyxedema, medicine, Humans, Immunologic Factors, Glucocorticoids, Lenalidomide, Aged, Retrospective Studies, Skin, business.industry, Immunoglobulins, Intravenous, Plasmapheresis, Cell Biology, Hematology, Middle Aged, medicine.disease, 3. Good health, 030104 developmental biology, medicine.anatomical_structure, Leonine facies, Female, Transcriptome, business, Refractory cytopenia with multilineage dysplasia, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology, 030215 immunology, medicine.drug
Abstract

International audience; Scleromyxedema is a rare skin and systemic mucinosis that is usually associated with monoclonal gammopathy (MG). In this French, multicenter, retrospective study of 33 patients, we investigated the clinical and therapeutic features of MG-associated scleromyxedema. Skin molecular signatures were analyzed using a transcriptomic approach. Skin symptoms included papular eruptions (100%), sclerodermoid features (91%), and leonine facies (39%). MG involved an IgG isotype in all patients, with a predominant λ light chain (73%). Associated hematologic malignancies were diagnosed in 4/33 patients (12%) (smoldering myeloma, n=2; chronic lymphoid leukemia, n=1; and refractory cytopenia with multilineage dysplasia n=1). Carpal tunnel syndrome (33%), arthralgia (25%) and dermato-neuro syndrome (DNS) (18%) were the most common systemic complications. One patient with mucinous cardiopathy died of acute heart failure. Intravenous immunoglobulin (HDIVig) treatment alone or in combination with steroids appeared to be quite effective in nonsevere cases (clinical complete response achieved in 13/31 patients). Plasma cell-directed therapies using lenalidomide and/or bortezomib with dexamethasone and HDIVig led to a significant improvement in severe cases (HDIVig-refractory or cases with central nervous system or cardiac involvement). The emergency treatment of DNS with combined plasmapheresis, HDIVig, and high-dose corticosteroids induced the complete remission of neurological symptoms in 4/5 patients. Quantitative reverse transcriptase-PCR (RT-PCR) analysis of 6 scleromyxedema skin samples showed significantly higher profibrotic pathway levels (transforming growth factor β (TGFβ) and collagen-1) than in healthy skin. Prospective studies targeting plasma cell clones and/or fibrotic pathways are warranted for long-term scleromyxedema management.

Published
2020
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3. Multiple Antigenic Peptide-Based Vaccines Targeting Ixodes ricinus Neuropeptides Induce a Specific Antibody Response but Do Not Impact Tick Infestation

Author

Nicolas Versillé, Martine Cote, Ladislav Šimo, Consuelo Almazán, Lisa Fourniol, Sabine Rakotobe, Sarah Bonnet, Jennifer Richardson, Jennifer Maye, Sandy Peltier, Jeremie Bornères, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Air Liquide Healthcare, Air Liquide [Siège Social], Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ERGANEO (ex SATTIdF Innov), CEVA SantéAnimale, Animal Health Department of INRAE, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Microbiology (medical), Ixodes ricinus, Tick infestation, SIFamide, 030231 tropical medicine, lcsh:Medicine, Biology, Tick, 03 medical and health sciences, 0302 clinical medicine, Antigen, Immunity, parasitic diseases, medicine, Immunology and Allergy, Anaplasma, Nymph, Molecular Biology, 030304 developmental biology, 0303 health sciences, General Immunology and Microbiology, lcsh:R, vaccines, medicine.disease, biology.organism_classification, 3. Good health, MIP, Vaccination, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Immunology, multiple antigenic peptides
Abstract

International audience; Synthetic peptide vaccines were designed to target the neuropeptides innervating Ixodes ricinus salivary glands and hindgut and they were tested for their capacity to afford protective immunity against nymphs or larvae and Anaplasma phagocytophilum-infected nymph infestation, in mice and sheep, respectively. In both models, the assembly of SIFamide (SIFa) or myoinhibitory peptide (MIP) neuropeptides into multiple antigenic peptide constructs (MAPs) elicited a robust IgG antibody response following immunization. Nevertheless, no observable detrimental impact on nymphs was evidenced in mice, and, unfortunately, the number of engorged nymphs on sheep was insufficient for firm conclusions to be drawn, including for bacterial transmission. Regarding larvae, while vaccination of the sheep did not globally diminish tick feeding success or development, analyses of animals at the individual level revealed a negative correlation between anti-SIFa and MIP antibody levels and larva-to-nymph molting success for both antigens. Our results provide a proof of principle and precedent for the use of MAPs for the induction of immunity against tick peptide molecules. Although the present study did not provide the expected level of protection, it inaugurates a new strategy for protection against ticks based on the immunological targeting of key components of their nervous system.

Published
2020
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