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Made available in DSpace on 2021-06-25T11:00:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Photoperiod is the main cue that controls seasonal reproduction in deer from temperate regions, like secretion of testosterone by the testis, neck enlargement, and testis development. However, little is known about its effect on the reproduction of tropical deer species. This research aimed to assess the potential seasonality of the brown brocket deer (Mazama gouazoubira) by melatonin treatment. Reproductive parameters such as testicular volume, neck circumference, and semen quality were evaluated from day 0 to 90, and fecal androgen metabolites (FAM) from day −28 to 91. Melatonin treatment was administered from day 0 to 60. In total, seven individuals were used in the study. Four animals were allocated to the treated group (TG) and three to the control group (CG). The neck circumference and some seminal parameters did not show a significant difference between CG and TG and along the experimental period. High FAM levels were observed for the TG on days 7, 14, 21, 35, 84, and 91 (p < 0.05). The mean testicular volume was reduced from day 0 to 30 (p < 0.05) and from day 0 to 90 (p < 0.05), with no increase in the mean testicular volume from day 30 to 60 (p > 0.05). Sperm motility was the highest on day 60 compared with day 0 (p < 0.05). Although not statistically different, membrane integrity and major defects tended to be higher and lower, respectively, in the TG compared with the CG. For these parameters, the incremental change (%) per individual showed that males from the TG tended to have a higher percentage during the melatonin treatment, hence melatonin might affect these seminal characteristics. These findings suggest that exogenous melatonin might enhance FAM levels and sperm motility. Veterinary Science Postgraduate Program School of Agricultural and Veterinary Studies (FCAV) São Paulo State University (UNESP), Jaboticabal Animal Science Postgraduate Program School of Agricultural and Veterinary Studies (FCAV) São Paulo State University (UNESP), Jaboticabal Deer Research and Conservation Center (NUPECCE) School of Agricultural and Veterinary Studies (FCAV) São Paulo State University (UNESP), Jaboticabal Veterinary Science Postgraduate Program School of Agricultural and Veterinary Studies (FCAV) São Paulo State University (UNESP), Jaboticabal Animal Science Postgraduate Program School of Agricultural and Veterinary Studies (FCAV) São Paulo State University (UNESP), Jaboticabal Deer Research and Conservation Center (NUPECCE) School of Agricultural and Veterinary Studies (FCAV) São Paulo State University (UNESP), Jaboticabal

Made available in DSpace on 2021-06-25T10:27:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-01 Central European Institute of Technology ASCRS Research Foundation Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grantová Agentura České Republiky The genus Mazama stands out among the Neotropical deer due to their wide intra and interspecific karyotypic diversification, which is associated with an accentuated chromosomal fragility. There are reports of heterozygous Robertsonian translocation (RT) carriers in a free-range population of Mazama gouazoubira (brown brocket deer), as well as in captive animals of this and other species of the genus. To analyze possible negative impacts of heterozygous chromosome rearrangements on reproductive fitness of the carriers, we performed an analysis of sperm meiotic segregation in four brown brocket bucks, carriers of a rob(4;16), and compared the results with those of a normal buck. We established a reliable FISH and sperm-FISH protocol for the brown brocket deer using bovine (Bos taurus; diploid number, 2n = 60) whole chromosome painting (WCP) and BAC probes. Using BAC probes, we revealed the presence of a paracentric inversion (PAI) of the fused chromosome 4 in two of the four analyzed RT carriers. The mean frequency of normal/balanced sperm in the translocation carriers was significantly lower than in the normal buck (94.78% vs 98.40%). The mean value of total unbalanced spermatozoa was almost doubled in the RT/PAI carriers (6.68%) when compared to RT carriers (3.76%), but the difference was not statistically significant. This study demonstrated the efficiency of FISH with bovine WCP and BAC probes in the characterization of chromosome rearrangements and gametic segregation patterns in brown brocket deer. Our results indicate a low to moderate increase in the rates of unbalanced meiotic segregation products in brown brocket bucks heterozygous for RT and RT/PAIs. Núcleo de Pesquisa e Conservação de Cervídeos Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – NUPECCE/FCAV/UNESP Central European Institute of Technology-Veterinary Research Institute Núcleo de Pesquisa e Conservação de Cervídeos Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – NUPECCE/FCAV/UNESP Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica: 116-2017-FONDECYT FAPESP: 2017/07014-8 FAPESP: 2019/06940-1 Grantová Agentura České Republiky: 20–22517J

Made available in DSpace on 2021-06-25T11:12:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01. Added 1 bitstream(s) on 2021-07-15T15:24:12Z : No. of bitstreams: 1 S1984-31432020000400206.pdf: 543405 bytes, checksum: bec495f1bb122898b83e030f631bac84 (MD5) Deer are sensitive to stressful stimuli by handling and their reproductive physiology could be altered by these procedures, making it necessary to develop less invasive protocols for ART. Melengestrol acetate (MGA), a synthetic progestin administered orally, appears as an alternative for estrous synchronization protocols (ESP), such as reported in cattle. Firstly, we compared two MGA doses (0.5 and 1.0 mg/day/animal), which would have suppression effect in estrous behavior (EB). Eight females were randomly and equally distributed in Group 1 (G1) and Group 2 (G2), which received 0.5 and 1.0 mg/day/animal respectively for 15 days (D1 to D15). Two cloprostenol (CP) applications were performed on D0 and D11. Estrus detection (ED) was performed every day. All females from G1 displayed estrus during treatment period, whereas all females from G2 displayed estrus after treatment, suggesting a suppressive effect of 1.0mg in the EB. Once the suppressive MGA dose (1.0 mg) was defined, we used this dose for assessing ESP. The same eight females received 1.0 mg/animal for eight days (D-8 to D-1), followed by 0.25 mg of estradiol benzoate on D-8 and 265 μg of CP on D0. Feces for fecal progesterone metabolites (FPM) measurement were collected from D0 until seven days after the last day of estrus. Seven females displayed estrus between 12 and 72 h after CP application, which was followed by a significant increase in FPM levels (except female MG6), suggesting the formation of corpus luteum. After ED, females were placed with a fertile male to assess the fertility of the protocol. Pregnancy was confirmed by ultrasound 30 days after mating in 3/6 individuals. Although the low effectiveness of MGA protocol, it should be considered as a promising alternative in deer ESP since this protocol has less stressful effect on the animal during reproductive management when compared to other ESP Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE) Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP) Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE) Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)

Made available in DSpace on 2018-12-11T17:04:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-01 The Amazonian brown brocket deer (Mazama nemorivaga) is an endemic species of the Amazon rainforest region, which has suffered constant threats due to hunting and increasing deforestation. Artificial insemination (AI), combined with genomic banks, is considered an important tool for maintaining conservation programs of endangered species; however, the number of live offspring born from AI in non-domesticated cervids is extremely low. Thus, studies designed to develop, adapt, or enhance AI techniques are of fundamental importance. This report describes a successful transposition of the cervix with semen deposition in the uterine lumen of a M. nemorivaga female, based on the transcervical AI technique used in sheep, as well as using specific tools develop for IA in small ruminants, which have resulted in the birth of a healthy male fawn. Department of Preventive Veterinary Medicine and Animal Reproduction College of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP) Deer Research and Conservation Center (NUPECCE) Department of Animal Science FCAV-UNESP Department of Clinical and Veterinary Surgery FCAV-UNESP Department of Preventive Veterinary Medicine and Animal Reproduction College of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP) Deer Research and Conservation Center (NUPECCE) Department of Animal Science FCAV-UNESP Department of Clinical and Veterinary Surgery FCAV-UNESP

Made available in DSpace on 2018-11-26T17:44:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-25 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The marsh deer is an endangered species from the marshlands of central South America. This study aimed to characterize certain aspects of the reproductive physiology of marsh deer hinds, including the duration and fecal progestins profile of the estrous cycle, pregnancy and post-partum periods, and evaluate the effect of cloprostenol administration on this species. The experimental group consisted of six females and one fertile male marsh deer. During monitoring of the estrous cycle, the fresh fecal samples were collected daily and, during pregnancy, they were collected twice weekly. The hormonal profile obtained from daily fecal samples indicated that the mean duration of the estrous cycle was 21.3 +/- 1.3 days (6.4 days inter-luteal phase and 14.8 days luteal phase; n = 16 estrous cycles). The mean concentration of fecal progestins in the inter-luteal phase was 834 +/- 311 ng g(-1), in the luteal phase was 3979 +/- 1611 ng g(-1), value between them was 1457 ng g(-1). No significant difference in fecal estrogen concentrations was determined during the estrous cycle. The corpora luteum was not responsive to cloprostenol until Day 6 of the estrous cycle, the period previously described as the inter-luteal phase. Half the females became pregnant following treatment with cloprostenol and two others were fertilized in their natural estrous cycle. Four females delivered fawns, and the mean duration of pregnancy was 253 +/- 4 days. Fecal progestin concentrations were similar to those of the estrous cycle during the first 11 weeks of pregnancy and increased significantly (> 15250 ng g(-1)) thereafter, providing a presumptive diagnosis guideline. Within 60 days of post-partum analyses, 75% of the deer exhibited behavioural estrus and/or ovarian activity. This study generated a broader understanding of the marsh deer species concerning the production of consistent data related to its reproduction. This knowledge can be used to assist the reproductive management of this species and, consequently, to promote its conservation. Univ Estadual Paulista, Fac Ciencias Agr & Vet, NUPECCE Nucleo Pesquisa & Conservacao Cervideos, Deer Res & Conservat Ctr,Dept Zootecnia, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, NUPECCE Nucleo Pesquisa & Conservacao Cervideos, Deer Res & Conservat Ctr,Dept Zootecnia, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil