1. CD147 expression in peritoneal injury
- Author
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M. Dominik Alscher, Rudolf P. Wüthrich, Joerg Latus, Jin Chen, Stephan Segerer, Harald Seeger, Ilka Edenhofer, Daniel Kitterer, Dagmar Biegger, University of Zurich, and Segerer, Stephan
- Subjects
Male ,0301 basic medicine ,Nephrology ,medicine.medical_specialty ,Pathology ,Physiology ,Biopsy ,medicine.medical_treatment ,030232 urology & nephrology ,Adipose tissue ,610 Medicine & health ,Peritoneal dialysis ,Extracellular matrix ,03 medical and health sciences ,Peritoneal cavity ,2737 Physiology (medical) ,0302 clinical medicine ,Physiology (medical) ,Internal medicine ,medicine ,Humans ,10035 Clinic for Nephrology ,Myofibroblasts ,Aged ,Retrospective Studies ,2727 Nephrology ,business.industry ,Encapsulating peritoneal sclerosis ,Peritoneal Fibrosis ,1314 Physiology ,Middle Aged ,Mesothelium ,030104 developmental biology ,medicine.anatomical_structure ,CD147 ,Emmprin ,Basigin ,Immunohistochemistry ,Female ,EPS ,Peritoneum ,business ,Peritoneal Dialysis ,Myofibroblast - Abstract
Peritoneal injury is an important cause of technical failure of long-term peritoneal dialysis (PD). Encapsulating peritoneal sclerosis (EPS) is a severe complication of long-term PD with potentially life threatening consequences. CD147 is a glycoprotein with diverse functions including modulation of extracellular matrix via induction of matrix metalloproteinases, cell adhesion, and regulation of immune reactions. We hypothesized that CD 147 plays a role in the peritoneal cavity. In this retrospective study, we localized CD147 by immunohistochemistry in peritoneal biopsies from uremic patients not on PD (n = 8), on PD without signs of EPS (n = 7), and in biopsies in patients with the diagnosis of EPS (n = 7). Double immunofluorescence was used to co-localize α-smooth-muscle actin (α-SMA) and CD147 in selected biopsies from each group. Expression was scored semi-quantitatively. In biopsies from uremic controls, CD147 was prominently expressed in mesothelial cells, focally between fat cells and by some perivascular cells. In patients on PD, a similar distribution was present (although mesothelium was rarely conserved), with some focal accentuation. In EPS, layers of fibroblastic cells were positive for CD147. EPS biopsies demonstrated a significantly higher score in a blinded evaluation, compared to uremic patients. Cells expressing CD147 were α-SMA positive myofibroblasts as demonstrated by double immunofluorescence. Mean CD147 scores did not differ between patients with different transporter status. This is the first study demonstrating CD147 on a major part of fibroblastic cells in EPS. Future studies need to address the role of these cells in this severe complication of long-term PD.
- Published
- 2017