1. Assessment of mTOR pathway molecules during implantation in rats
- Author
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Sevinc Inan, Ece Onur, Gulperi Oktem, Gülçin Ekizceli, Kemal Ozbilgin, Department of Histology and Embryology, Uludag University Faculty of Medicine, Bursa, Turkey, Department of Histology and Embryology, Izmir University of Economics, Faculty of Medicine, Izmir, Turkey, Department of Histology and Embryology, Ege University, Faculty of Medicine, Izmir, Turkey, Department of Medical Biochemistry, Celal Bayar University, Faculty of Medicine, Manisa, Turkey, Department of Histology and Embryology, Celal Bayar University, Faculty of Medicine, Manisa, Turkey, Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı., Ekizceli, Gülçin, AAZ-2915-2020, and AAP-3523-2020
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Physiology ,Angiogenesis ,Expression ,mTORC1 ,mTORC2 ,ERK1 ,Western blotting ,0302 clinical medicine ,Pregnancy ,Nidation ,Decidual cells ,Phosphorylation ,030219 obstetrics & reproductive medicine ,Biotechnology & applied microbiology ,Signaling pathway ,TOR Serine-Threonine Kinases ,Target of rapamycin kinase ,General Medicine ,Select nutrients ,Immunohistochemistry ,Implantation ,Stem-cells ,Medical Laboratory Technology ,Animals ,3 Phosphoinositide Dependent Protein Kinase ,Phosphatidylinositols ,Female ,Signal Transduction ,Cell biology ,MTOR protein, human ,Cell signaling ,medicine.medical_specialty ,Histology ,Blotting, Western ,Biology ,03 medical and health sciences ,Complex ,Internal medicine ,medicine ,Embryo Implantation ,Protein kinase B ,PI3K/AKT/mTOR pathway ,MTORC1 ,PAKT1/2/3 ,Vaginal Smears ,P70 s6 kinase ,MTORC2 ,Vagina smear ,Animal ,Uterus ,Rats ,Glucose ,Metabolism ,030104 developmental biology ,Endocrinology ,Cancer research ,Rat ,Growth-factors - Abstract
Mammalian target of rapamycin (mTOR) is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. We investigated the role of mTOR and other signaling molecules in the rat uterus during implantation. Female pregnant rats were divided into three groups: embryonic days (ED) 4.5, 5.5 and 6.5 according to vaginal smears. Immunohistochemical staining of mTORC1, mTORC2, IGF1, PI3K, pAkt1/2/3, ERK1 and pERK1/2 was performed on formalin fixed, paraffin embedded uterine tissue samples. pAkt1/2/3 and ERK1 also were analyzed using western blotting. We found that PI3K/Akt/mTOR and ERK/pERK were increased during the implantation period. Different amounts of mTORC1, mTORC2, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 were expressed in luminal epithelium, decidual cells, embryoblast and trophoblast cells during implantation. We suggest that mTOR and associated signaling molecules may participate in implantation. Celal Bayar Üniversitesi Bilimsel Araştırma Proje Komitesi - 2011/038
- Published
- 2017
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