1. Paired box 7 inhibits differentiation in 3T3-L1 preadipocytes
- Author
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Mako Nakamura, Ryo Ebihara, Yuko Takuma, Wakana Izumi, Wataru Mizunoya, and Ryuichi Tatsumi
- Subjects
0301 basic medicine ,Receptor, Platelet-Derived Growth Factor alpha ,Down-Regulation ,Gene Expression ,Fatty Acid-Binding Proteins ,Muscle Development ,MyoD ,Mice ,03 medical and health sciences ,3T3-L1 Cells ,Adipocytes ,Animals ,Myocyte ,MyoD Protein ,Adipogenesis ,030102 biochemistry & molecular biology ,Myogenesis ,Chemistry ,PAX7 Transcription Factor ,Cell Differentiation ,Lipid Droplets ,General Medicine ,musculoskeletal system ,Cell biology ,PPAR gamma ,030104 developmental biology ,Myogenic regulatory factors ,MYF5 ,Myogenic Regulatory Factor 5 ,PAX7 ,Stem cell ,General Agricultural and Biological Sciences ,tissues - Abstract
Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.
- Published
- 2018
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