1. Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities
- Author
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Rubén Ayala Suárez, Juan Miguel Mancera, Hany M. Ibrahim, Antonio Astola González, Gonzalo Martínez-Rodríguez, Asmaa Galal-Khallaf, Khaled Mohammed-Geba, Juan Antonio Martos-Sitcha, Ministerio de Asuntos Exteriores y Cooperación (España), Academy of Scientific Research and Technology (Egypt), and Universidad de Cádiz
- Subjects
0301 basic medicine ,Mediterranean climate ,Fish Proteins ,medicine.medical_specialty ,endocrine system ,Salinity ,DNA, Complementary ,Physiology ,medicine.medical_treatment ,Acclimatization ,Aquatic Science ,Biology ,Argyrosomus regius ,Biochemistry ,Perciformes ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Insulin-Like Growth Factor I ,Phylogeny ,Osmotic concentration ,Base Sequence ,prl ,Growth factor ,actb ,04 agricultural and veterinary sciences ,General Medicine ,Euryhaline ,biology.organism_classification ,Prolactin ,gh ,igf1 ,qPCR ,030104 developmental biology ,Endocrinology ,Growth Hormone ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,hormones, hormone substitutes, and hormone antagonists - Abstract
Aquaculture industry in the Mediterranean region exhibits a growing interest for the Mediterranean meager Argyrosomus regius. Some preliminary works showed a good growth performance of the species in nearly isosmotic salinities. However, the patterns of alteration of prolactin (Prl) as well as growth hormone (Gh)/insulin growth factor-1 (Igf1) axis at the molecular level are not yet described in this species. Therefore, we cloned and sequenced partial cDNAs for pituitary prolactin (prl) and growth hormone (gh), hepatic insulin-like growth factor (igf1), and β-actin (actb). Expression patterns of these transcripts were tested in juveniles of A. regius acclimated to four different environmental salinities: (1) 5 ‰ (hyposmotic); (2) 12 ‰ (isosmotic); (3) 38 ‰ (hyperosmotic; seawater control); and (4) 55 ‰ (extremely hyperosmotic). All investigated transcripts shared high sequence identities with their counterparts in other perciformes. prl mRNA levels showed inverse pattern with increasing salinities. gh mRNA enhanced significantly in both 12 and 55 ‰ salinity groups in comparison with the control group, while igf1 showed its maximum expression levels under the nearly isosmotic environment. The results indicated clear sensitivity of prl, gh and igf1 to changes in environmental salinity, which can possibly control the euryhalinity capacity of this species., This work was carried out as a part of the Spanish-Egyptian joint project AP/039755/11(Development of molecular, physiological and immunological biomarkers for the detection of stress related to the Mediterranean meager aquaculture Argyrosomus regius) awarded from the Spanish agency of international cooperation (Agencia Española de Cooperación Internacional, AECID) and the Egyptian Academy of Scientific Research and Technology, to Juan Miguel Mancera (Universidad de Cádiz, Spain) and Khaled Mohammed-Geba (Menofia University, Egypt).
- Published
- 2016