1. WDHD1 facilitates G1 checkpoint abrogation in HPV E7 expressing cells by modulating GCN5
- Author
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Qianqian Zhao, Mingyu Ji, Fang Zhang, Yunshan Wang, Juanjuan Tian, Yatian Hong, Yunying Zhou, Fengyan Pei, Jason J. Chen, Yingshuo Sun, and Xiao Wang
- Subjects
0301 basic medicine ,Genome instability ,Cancer Research ,HPV ,Cell cycle checkpoint ,Carcinogenesis ,Papillomavirus E7 Proteins ,WDHD1 ,Biology ,medicine.disease_cause ,Transfection ,lcsh:RC254-282 ,Genomic Instability ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Humans ,p300-CBP Transcription Factors ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Gene knockdown ,Human papillomavirus 16 ,DNA synthesis ,G1 arrest ,Papillomavirus Infections ,Cell cycle ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,G1 Phase Cell Cycle Checkpoints ,P-Akt ,Cell biology ,DNA-Binding Proteins ,030104 developmental biology ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Gene Knockdown Techniques ,Proto-Oncogene Proteins c-akt ,Research Article ,GCN5 ,DNA Damage - Abstract
Background Genomic instability is a hallmark of cancer. The G1 checkpoint allows cells to repair damaged DNA that may lead to genomic instability. The high-risk human papillomavirus (HPV) E7 gene can abrogate the G1 checkpoint, yet the mechanism is still not fully understood. Our recent study showed that WDHD1 (WD repeat and high mobility group [HMG]-box DNA-binding protein 1) plays a role in regulating G1 checkpoint of E7 expressing cells. In this study, we explored the mechanism by which WDHD1 regulates G1 checkpoint in HPV E7 expressing cells. Methods NIKS and RPE1 derived cell lines were used. Real-time PCR, Rescue experiment, FACS and BrdU labeling experiments were performed to examine role of GCN5 in G1 checkpoint abrogation in HPV-16 E7 expressing cells. Results In this study, we observed that WDHD1 facilitates G1 checkpoint abrogation by modulating GCN5 in HPV E7 expressing cells. Notably, depletion of WDHD1 caused G1 arrest while overexpression of GCN5 rescued the inhibitory effects of WDHD1 knockdown on G1/S progression. Furthermore, siWDHD1 significantly decreased cell cycle proliferation and DNA synthesis that was correlated with Akt phosphorylation (p-Akt), which was reversed by GCN5 overexpression in HPV E7 expressing cells. Conclusions In summary, our data identified a WDHD1/GCN5/Akt pathway leading to the abrogation of G1 checkpoint in the presence of damaged DNA, which may cause genomic instability and eventually HPV induced tumorigenesis.
- Published
- 2020