1. Engineering cancer stem-like cells from normal human lung epithelial cells
- Author
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Machiko Itoh-Yagi, Ken Sasai, Etsuko Takao-Rikitsu, Tsuyoshi Akagi, Yukina Izumi, Taiko Sukezane, Harumi Nakagawa, Emmy Yanagita, and Tomoo Itoh
- Subjects
0301 basic medicine ,Lung Neoplasms ,Carcinogenesis ,Cellular differentiation ,lcsh:Medicine ,Squamous Cell Lung Carcinoma ,Lung and Intrathoracic Tumors ,Interleukin 22 ,Mice ,0302 clinical medicine ,Animal Cells ,Cancer Stem Cells ,Adenocarcinomas ,Medicine and Health Sciences ,lcsh:Science ,Cell Engineering ,Lung ,Cells, Cultured ,Mice, Inbred BALB C ,Multidisciplinary ,Adenocarcinoma of the Lung ,Stem Cells ,Squamous Cell Carcinomas ,Cell Differentiation ,Cell Transformation, Neoplastic ,Oncology ,030220 oncology & carcinogenesis ,Amniotic epithelial cells ,Neoplastic Stem Cells ,Female ,Cellular Types ,Research Article ,Tumour heterogeneity ,Mice, Nude ,Respiratory Mucosa ,Biology ,Carcinomas ,03 medical and health sciences ,Cancer stem cell ,medicine ,Adenocarcinoma of the lung ,Animals ,Humans ,Differentiated Tumors ,A549 cell ,lcsh:R ,Biology and Life Sciences ,Cancers and Neoplasms ,Cancer ,Cell Biology ,medicine.disease ,030104 developmental biology ,Cancer research ,lcsh:Q ,Secondary Lung Tumors ,Neoplasm Transplantation ,Developmental Biology - Abstract
It has been proposed that a subpopulation of tumour cells with stem cell-like characteristics, known as cancer stem cells (CSCs), drives tumour initiation and generates tumour heterogeneity, thus leading to cancer metastasis, recurrence, and drug resistance. Although there has been substantial progress in CSC research into many solid tumour types, an understanding of the biology of CSCs in lung cancer remains elusive, mainly because of their heterogeneous origins and high plasticity. Here, we demonstrate that engineered lung cancer cells derived from normal human airway basal epithelial cells possessed CSC-like characteristics in terms of multilineage differentiation potential and strong tumour-initiating ability. Moreover, we established an in vitro 3D culture system that allowed the in vivo differentiation process of the CSC-like cells to be recapitulated. This engineered CSC model provides valuable opportunities for studying the biology of CSCs and for exploring and evaluating novel therapeutic approaches and targets in lung CSCs.
- Published
- 2017