1. Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs
- Author
-
Yizhi Xiao, Maria Pia Canales, Walter L. Siqueira, Lina María Marín, and Karla Tonelli Bicalho Crosara
- Subjects
0301 basic medicine ,Bacterial Diseases ,Saliva ,Teeth ,Proteome ,Orthodontic Brackets ,Gene Expression ,Proteomics ,Biochemistry ,0302 clinical medicine ,Medical Conditions ,Spectrum Analysis Techniques ,Caries ,Medicine and Health Sciences ,Chromatography, High Pressure Liquid ,Multidisciplinary ,Chemistry ,Bracket ,Separation Processes ,Infectious Diseases ,Metals ,visual_art ,visual_art.visual_art_medium ,Medicine ,Structural Proteins ,Anatomy ,Research Article ,Spectrometry, Mass, Electrospray Ionization ,Science ,Oral Medicine ,Orthodontics ,Research and Analysis Methods ,Metal ,03 medical and health sciences ,Adsorption ,X-ray photoelectron spectroscopy ,DNA-binding proteins ,Genetics ,Humans ,Gene Regulation ,Salivary Proteins and Peptides ,Distillation ,Biology and life sciences ,Mucin ,Proteins ,030206 dentistry ,X-Ray Photoelectron Spectroscopy ,Regulatory Proteins ,030104 developmental biology ,Durapatite ,Jaw ,Electron Beam Spectrum Analysis Techniques ,Digestive System ,Head ,Nuclear chemistry ,Transcription Factors - Abstract
The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired bracket pellicle was characterized after brackets were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs were used as a control. Acquired pellicle was harvested from the HA discs (n = 12) and from the metallic brackets (n = 12). Proteomics based on mass spectrometry technology was used for salivary protein identification and characterization. Results showed that most of the proteins adsorbed on the surface of orthodontic metallic brackets and on the HA discs were identified specifically to each group, indicating a small overlapping between the salivary proteins on each study group. A total of 311 proteins present on the HA discs were unique to this group while 253 proteins were unique to metallic brackets, and only 45 proteins were common to the two groups. Even though most proteins were unique to each study group, proteins related to antimicrobial activity, lubrication, and remineralization were present in both groups. These findings demonstrate that the salivary proteins adsorbed on the bracket surface are dependent on the material molecular composition.
- Published
- 2021