1. EcoTILLING revealed SNPs in GhSus genes that are associated with fiber- and seed-related traits in upland cotton
- Author
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Kang-Sheng Deng, Yuan-Ming Zhang, Yan-Da Zeng, Tianzhen Zhang, Jun-Ling Sun, Xiongming Du, Su-Hong Bu, Baoliang Zhou, and Tao Tao
- Subjects
0106 biological sciences ,0301 basic medicine ,Linkage disequilibrium ,Quantitative Trait Loci ,Single-nucleotide polymorphism ,Quantitative trait locus ,Gossypium ,Polymorphism, Single Nucleotide ,01 natural sciences ,Linkage Disequilibrium ,Article ,03 medical and health sciences ,Cotton Fiber ,Allele ,Gene ,Alleles ,Molecular breeding ,Genetics ,Multidisciplinary ,biology ,biology.organism_classification ,Phenotype ,030104 developmental biology ,Seeds ,Epistasis ,Genome, Plant ,010606 plant biology & botany - Abstract
Cotton is the most important textile crop in the world due to its cellulose-enriched fibers. Sucrose synthase genes (Sus) play pivotal roles in cotton fiber and seed development. To mine and pyramid more favorable alleles for cotton molecular breeding, single nucleotide polymorphisms (SNPs) of GhSus family genes were investigated across 277 upland cotton accessions by EcoTILLING. As a result, a total of 24 SNPs in the amplified regions of eight GhSus genes were identified. These SNPs were significantly associated with at least one fiber- or seed-related trait measured in Nanjing, Anyang and Kuche in 2007–2009. Four main-effect quantitative trait nucleotides (QTNs) and five epistatic QTNs, with 0.76–3.56% of phenotypic variances explained by each QTN (PVE), were found to be associated with yield-related traits; six epistatic QTNs, with the 0.43–3.48% PVE, were found to be associated with fiber quality-related traits; and one main-effect QTN and one epistatic QTN, with the PVE of 1.96% and 2.53%, were found to be associated with seed oil content and protein content, respectively. Therefore, this study provides new information for molecular breeding in cotton.
- Published
- 2016
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