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To investigate the prevalence and genetic diversity of Grapevine berry inner necrosis virus (GINV) in China, 195 grapevine samples from 15 Chinese provinces and regions were tested using reverse-transcription polymerase chain reaction. The samples included symptomatic and asymptomatic cultivars, with 35.9% (70 of 195) of samples testing positive for GINV. Seventeen samples had obvious ring spot symptoms, and 94.1% (16 of 17) tested positive for GINV, suggesting that GINV may be highly associated with the ring spot symptom. The genetic diversity of GINV isolates was analyzed based on the partial nucleotide and amino acid sequences of the coat protein (CP) and movement protein (MP) genes. Phylogenetic analyses of the MP and CP gene sequences divided the GINV isolates into three groups. The majority of the Chinese isolates were in groups 1 and 2, and only one Chinese isolate, along with a previously reported Japanese isolate, was in group 3. This is the first report on the genetic diversity of GINV isolates and their prevalence and distribution in China.

Recently, a novel virus tentatively named grapevine fabavirus (GFabV) was reported in North America, it currently classified as a putative member of the genus Fabavirus (Al Rwahnih et al. 2016). Currently, its effects on grapevines remain unknown. In this study, leaves of three virus-eliminating ‘Beta’ grapevines (Vitis riparia×V.labrusca) showed chlorotic mottling and deformation after being grafted with ‘Ruby seedless’ grapevine (Vitis vinifera L.) scion. Before grafting, the ‘Beta’ and ‘Ruby seedless’ grapevines had been screened by RT-PCR for Grapevine leafroll-associated virus -1-4 and 7 (GLRaV-1-4 and 7), Grapevine fanleaf virus, Grapevine rupestris stem pitting-associated virus, Grapevine fleck virus, Grapevine virus A (GVA), GVB, GVE, Grapevine pinot gris virus, and Grapevine berry inner necrosis virus. No viruses were detected in the ‘Beta’ grapevines, except for GLRaV-3 in the ‘Ruby seedless’ grapevine. Additionally, small RNAs from the diseased ‘Beta’ leaves were sequenced as described previous...