1. Facilitating Imaging Mass Spectrometry of Microbial Specialized Metabolites with METASPACE
- Author
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René De Mot, Veronika Saharuka, Lachlan Stuart, Theodore Alexandrov, Don D. Nguyen, Vittorio Venturi, Kinga Lubowiecka, Vitaly Kovalev, and Massimo Del Prete
- Subjects
0301 basic medicine ,Biochemistry & Molecular Biology ,Science & Technology ,010405 organic chemistry ,Chemistry ,Communication ,Endocrinology, Diabetes and Metabolism ,Metabolite ,Data interpretation ,Computational biology ,01 natural sciences ,Biochemistry ,Microbiology ,imaging mass spectrometry ,Mass spectrometry imaging ,QR1-502 ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,spatial metabolomics ,030104 developmental biology ,microbial natural products ,Molecular Biology ,Life Sciences & Biomedicine - Abstract
Metabolite annotation from imaging mass spectrometry (imaging MS) data is a difficult undertaking that is extremely resource intensive. Here, we adapted METASPACE, cloud software for imaging MS metabolite annotation and data interpretation, to quickly annotate microbial specialized metabolites from high-resolution and high-mass accuracy imaging MS data. Compared with manual ion image and MS1 annotation, METASPACE is faster and, with the appropriate database, more accurate. We applied it to data from microbial colonies grown on agar containing 10 diverse bacterial species and showed that METASPACE was able to annotate 53 ions corresponding to 32 different microbial metabolites. This demonstrates METASPACE to be a useful tool to annotate the chemistry and metabolic exchange factors found in microbial interactions, thereby elucidating the functions of these molecules. ispartof: METABOLITES vol:11 issue:8 ispartof: location:Switzerland status: published
- Published
- 2021