1. Antioxidant, anti-inflammatory and healing potential of ethyl acetate fraction of Bauhinia ungulata L. (Fabaceae) on in vitro and in vivo wound model
- Author
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Ana Paula Negreiros Nunes Alves, Greyce Luri Sasahara, Juliana Navarro Ueda Yaochite, Paulo Germano de Carvalho, Andressa Almeida Albuquerque, Aparecida Tiemi Nagao-Dias, Said Gonçalves da Cruz Fonseca, Thiago Dias de Vasconcelos Araújo, Leôncio Mesquita de Sousa, Jarbas Lima de Carvalho, Gilvandete M. P. Santiago, and Raphael de Oliveira Rodrigues
- Subjects
0301 basic medicine ,Male ,medicine.drug_class ,Acrylic Resins ,Anti-Inflammatory Agents ,Pharmacology ,Acetates ,Anti-inflammatory ,Antioxidants ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,In vivo ,Genetics ,medicine ,Animals ,Humans ,Molecular Biology ,Skin ,A549 cell ,Wound Healing ,Chemistry ,Plant Extracts ,Surgical wound ,Cell migration ,Fabaceae ,General Medicine ,Bauhinia ungulata ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,A549 Cells ,030220 oncology & carcinogenesis ,Bauhinia ,Wound healing ,Phytotherapy - Abstract
The present work aimed to investigate the antioxidant, anti-inflammatory and wound healing potential of ethyl acetate fraction from Bauhinia ungulata L. (FABU) on in vitro and in vivo models. Wound healing assay using human lung adenocarcinoma A549 cell line was employed to evaluate the ability of FABU in modulating cell migration. In addition, a surgical wound model in C57BL/6 mice was used to study the healing potential of FABU incorporated into gel carbomer 940 (Carbopol®). Evaluation of lipid peroxidation, inflammatory and anti-inflammatory mediator gene expression, rate of wound closure, and histological analysis were done. FABU significantly reduced the gap area in in vitro wound healing assay, 24 h after treatment. In the animal model, FABU at 0.5% topically applied once-daily for 5 days to the surgical wounds significantly reduced the lesion area. Moreover, it significantly decreased the levels of lipid peroxidation in the lesions and decreased the relative gene expression levels of IL-1β and TNF-α in the injured region. In conclusion, our study suggests that Bauhinia ungulata can effectively promote the wound healing, probably by regulating the inflammatory environment during the early stages of the process.
- Published
- 2019