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Saline groundwater (SGW) is the underground saline water obtained from basalt layer through which seawater has infiltrated. SGW contains more than 10,000 mg/L dissolved salt solid, the value of which is less than that of seawater. As part of its safety test, single and repeated oral dose toxicity tests were conducted with male and female ICR mice for 14 days. In single oral dose test with dosage of 10, 30, and 50 mL/kg, no gross changes in appearance or mortality were observed. In repeated oral dose test with dosage of 8, 14, and 20 mL/kg, no significant changes in mortality or weights of body and organ were observed. Additional analysis of serum biochemical parameter and histopathology also indicated no meaningful change during the tests. When taken all together, these results show that no toxicity of SGW could be found with single and repeated toxicity tests. However, for final conclusion of safety, further toxicity studies need to be performed with animal and human subjects.

Although wheat is a common staple food in the world, some people suffer from a variety of wheat allergies. For example, wheat-dependent exercise-induced anaphylaxis is induced in the gastrointestinal tract by wheat proteins. Relatively high molecular weight proteins that are salt-insoluble induce many wheat allergies. In the present study, we investigated the induction of an allergy response using crude wheat proteins, which are relatively low molecular weight, salt-soluble proteins. The crude antigen used in this study was extracted using phosphate buffered saline. When the antigen extracts from various wheat cultivars were orally administered, differentiable degrees of allergy responses were observed as measured by serum IgE and histamine secretion compared to the control. Serum IgE levels increased following administration of three of the wheat extracts. This evidence suggests that a combination of salt-soluble wheat proteins could be antigens for the induction of various allergy responses.

Recently, the incidence of diabetes has increased steadily worldwide. Type 2 diabetes constitutes about 90% of all cases of diabetes, and it is associated with many complications. Currently, the drugs for therapy of type 2 diabetes are considerably limited owing to disadvantages such as side effects and high rate of secondary failure. To overcome these problems, it is necessary to develop effective therapeutic agents from safe natural products. Deep-sea water (DSW) is abundant in minerals such as potassium (K), calcium (Ca), and magnesium (Mg). Mg supplements are known for their usefulness in the treatment of type 2 diabetes. In this study, we examined the anti-diabetic effect of Mg salt extracts from DSW in a diabetic mouse model. We observed that the groups treated with Mg salt extract showed better response toward fasting blood glucose level and oral glucose tolerance test when compared to the positive control. Overall, the Mg salt extract improved the symptoms of impaired fasting glucose and glucose tolerance, suggesting that it can be used as an effective agent for the treatment of type 2 diabetes mellitus.

Allergies and indigestion problems have increased in our society with increasing wheat consumption. Among wheat proteins, omega-5 gliadins (ω5-gliadins) and high molecular weight (HMW)-glutenin are known to be some of the major factors responsible for an allergic response and indigestion. Certain low molecular weight (LMW)-glutenin subunits are also able to promote local inflammation, and share common epitopes with ω5-gliadins. It is known that different wheat cultivars have different combinations of HMW- and LMW-glutenins. We investigated the relationship between the composition of LMW-glutenins and the degree of allergic response or indigestion. The extent of allergic reaction and indigestion characteristics in response to six different wheat cultivars were compared. Patterns of the change of blood glucose level with time, which were employed to measure the indigestion, were quite different, depending on the type of wheat cultivars. The extent of allergic response, as measured with the histamine and IgE levels, was also quite different, depending on the type of wheat cultivars. In addition, the extents were not correlated with patterns of LMW-glutenin DNA and HMW/LMW glutenin protein of various wheat cultivars. The results from the present study suggest that some more other factors, together with factors studied above, need to be considered to better explain the physiological phenomena of variation in blood glucose level and allergic response.

Deep sea water (DSW) is located 100 to 500 m below the sea surface. DSW is widely used in various fields, and is an important source of minerals that can be used to treat mineral deficiency. In the present study, the oral toxicity of DSW-mineral extracts was determined using single-dose and 14-day repeated dose oral toxicity tests in ICR mice. For the single-dose oral toxicity tests, mineral extracts of magnesium (Mg) and calcium (Ca) at doses of 0, 6, 270, 810, and 1,350 mg/kg, respectively, were orally administered to mice once at the beginning of the experiment, and the mice were observed for 14 days. For the 14- day repeated dose oral toxicity tests, Mg and Ca mineral extracts at doses of 0, 3, 135, 405, 675 mg/kg, respectively, were orally administered to mice daily, and the mice were observed for 14 days. Various tests were performed including visual observation; analysis of relative organ weight, food intake, and organ weight; biochemical analysis, and histopathology. The results indicated that mortality and changes in appearance were not observed among differentially administered groups of male and female ICR mice during the experimental period. Differences in body weight gain, food intake, organ weight, and histopathology parameters were not significant between the control and mineral-administered groups. Some results of the biochemical analyses were significantly different, but showed no specific tendencies. Overall, no evidence of toxicity was observed from the oral administration of DSW extracts of Ca and Mg in ICR mice.