1. Deep Proteomic Insights into the Individual Short-Term Pellicle Formation on Enamel-An In Situ Pilot Study
- Author
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Matthias Hannig, Simone Trautmann, Volkhard Helms, Ahmad Barghash, Johanna Dudek, Claudia Fecher-Trost, Pascal Schalkowsky, Nicolas Künzel, and Judith Delius
- Subjects
0301 basic medicine ,In situ ,Adult ,Male ,Proteomics ,Saliva ,Time Factors ,Clinical Biochemistry ,Dental pellicle ,Pilot Projects ,Enamel Pellicle ,03 medical and health sciences ,Young Adult ,stomatognathic system ,Humans ,Bovine enamel ,Dental Pellicle ,Proteomic Profile ,030102 biochemistry & molecular biology ,Enamel paint ,Chemistry ,Tooth surface ,stomatognathic diseases ,030104 developmental biology ,Biochemistry ,visual_art ,visual_art.visual_art_medium ,Female - Abstract
Purpose Dental pellicle formation starts instantaneously after oral hygiene due to the adsorption of salivary proteins to all orally exposed surfaces. The pellicle acts as a physiological mediator, protects the tooth surface from mechanical damages and reduces acid-induced enamel demineralization. The aim of this pilot study is to identify and characterize individual proteomic profiles of the initial pellicle formed on dental enamel and to compare the profiles with the corresponding saliva to analyze specific adsorption patterns occurring during pellicle formation. Experimental design The 3-min pellicle of five subjects formed in situ on bovine enamel is eluted chemically and analyzed separately by nano-mass spectrometry. The analysis of the corresponding saliva is conducted in parallel. Results Up to 498 pellicle proteins and up to 1032 salivary proteins are identified on an individual level. Comparison of the salivary and pellicle protein profiles demonstrates the pellicle formation to be highly individual. Nineteen proteins are significantly enriched in the 3-min pellicle of all subjects and 22 proteins are significantly depleted indicating that pellicle formation relies on selective adsorption. Conclusions and clinical relevance The short-term enamel pellicle is composed of several hundreds of adsorbed salivary proteins and reveals a highly individual proteomic profile.
- Published
- 2019