1. Sugarcane mosaic virus remodels multiple intracellular organelles to form genomic RNA replication sites
- Author
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Zaifeng Fan, Zhifang Li, Xiangdong Li, Tao Zhou, Jipeng Xie, and Tong Jiang
- Subjects
Crops, Agricultural ,Cytoplasm ,Chloroplasts ,Potyvirus ,Endoplasmic Reticulum ,Poaceae ,Virus Replication ,Viral Proteins ,03 medical and health sciences ,Virology ,Turnip mosaic virus ,Endomembrane system ,Plant Diseases ,030304 developmental biology ,0303 health sciences ,Mosaic virus ,biology ,030306 microbiology ,Brassica napus ,food and beverages ,RNA ,Genomics ,General Medicine ,biology.organism_classification ,RNA silencing ,Sugarcane mosaic virus ,Host-Pathogen Interactions ,RNA, Viral ,Viral genome replication - Abstract
Positive-stranded RNA viruses usually remodel host endomembrane system to form virus-induced intracellular vesicles for replication during infections. The genus Potyvirus of Potyviridae represents the largest number of positive single-stranded RNA viruses and causes great damage on crop production worldwide. Though potyviruses have wide host ranges, each potyvirus infects relatively limited host species. Phylogenesis and host range analysis can divide potyviruses into monocot-infecting and dicot-infecting groups, suggesting that some infection mechanism, probably on replication may be distinct for each group. Comprehensive studies on the model dicot-infecting turnip mosaic virus indicated that the 6K2-induced replication vesicles are derived from endoplasmic reticulum (ER) and subsequently target chloroplasts for viral genome replication. However, we have no knowledge on the replication site of monocot-infecting potyviruses. In this study, we firstly show that the precursor 6K2-VPg-Pro polyproteins of dicot-infecting potyviruses and monocot-infecting potyviruses phylogenetically cluster in two separate groups. With a typical gramineae-infecting potyvirus sugarcane mosaic virus (SCMV), we found that SCMV replicative double-stranded RNA (dsRNA) forms aggregates in cytoplasm but does not associate with chloroplasts. SCMV 6K2-VPg-Pro-induced vesicles colocalize with replicative dsRNA. Moreover, SCMV 6K2-VPg-Pro-induced structures target multiple intracellular organelles including ER, Golgi apparatus, mitochondria and peroxisomes, and have no evident association with chloroplasts. In conclusion, SCMV remodels multiple intracellular organelles for its genomic RNA replication.
- Published
- 2021