1. Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
- Author
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Ivo Vieira de Sousa Neto, H. S. Selistre-de-Araujo, Amanda Lima Ruivo, João Luiz Quaglioti Durigan, Keico Okino Nonaka, Rita de Cássia Marqueti, Fabio Henrique Bogni, Juliana Oliveira de Araújo, and Gonçalo Carreiro de Farias Junior
- Subjects
Male ,medicine.medical_specialty ,matrix metalloproteinase ,skeletal system ,Lumbar vertebrae ,Matrix metalloproteinase ,Exercícios físicos ,Bone and Bones ,Extracellular matrix ,03 medical and health sciences ,Random Allocation ,0302 clinical medicine ,Downregulation and upregulation ,Internal medicine ,Physical Conditioning, Animal ,Medicine ,Animals ,Femur ,Zymography ,030212 general & internal medicine ,Tibia ,Sistema musculoesquelético ,senescent ,Rats, Wistar ,Original Research ,remodeling ,exercise ,business.industry ,Envelhecimento ,Resistance training ,Resistance Training ,General Medicine ,Metaloproteinase ,Adaptation, Physiological ,Extracellular Matrix ,Rats ,medicine.anatomical_structure ,Endocrinology ,Clinical Interventions in Aging ,Cancellous Bone ,Matrix Metalloproteinase 2 ,Geriatrics and Gerontology ,business ,030217 neurology & neurosurgery ,Remodelação - Abstract
Ivo Vieira de Sousa Neto,1 João Luiz Quaglioti Durigan,1,2 Gonçalo Carreiro de Farias Junior,2 Fabio Henrique Bogni,3 Amanda Lima Ruivo,1 Juliana Oliveira de Araújo,1 Keico Okino Nonaka,3 Heloísa Selistre-de-Araújo,3 Rita de Cássia Marqueti1,2 1Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil; 2Graduate Program in Rehabilitation Sciences, Universidade de Brasília, Distrito Federal, Brazil; 3Department of Physiological Sciences, Universidade Federal de São Carlos, São Paulo, BrazilCorrespondence: Ivo Vieira de Sousa Neto; Rita de Cássia MarquetiLaboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, BrazilEmail ivoneto04@hotmail.com; marqueti@gmail.comBackground: Aging decreases osteogenic ability, inducing harmful effects on the bone extracellular matrix (ECM), while exercise training has been indicated as a tool to counteract bone disorders related to advancing age. The modulation of bone ECM is regulated by several types of matrix metalloproteinase (MMP); however, MMP-2 activity in different trabecular bones in response to resistance training (RT) has been neglected. Remodeling differs in different bones under the application of the same mechanical loading. Thus, we investigated the effects of 12 weeks of RT on MMP-2 activity in the lumbar vertebra (L6), tibia, and femur of young (3 months) and older rats (21 months).Methods: Twenty Wistar rats were divided into four groups (five animals per group): young sedentary or trained and older sedentary or trained. The 12-week RT consisted of climbing a 1.1-m vertical ladder three times per week with progressive weights secured to the animals’ tails. The animals were killed 48 h after the end of the experimental period. The MMP-2 activity was assessed by the zymography method.Results: The aging process induced lower MMP-2 activity in the lumbar vertebrae and tibia (p=0.01). RT upregulated pro, intermediate, and active MMP-2 activity in the tibia of young rats (p=0.001). RT also upregulated pro and active MMP-2 activity in the lumbar vertebrae and tibia with advancing age (p=0.01). There was no significant difference (p> 0.05) between groups for MMP-2 of the femur, regardless of age and RT.Conclusion: The aging process impairs MMP-2 activity, but RT is a potential therapeutic approach to minimize the deleterious effects of ECM degeneration in different aged bones. Distinct MMP-2 responses to exercise training may result in specific remodeling processes.Keywords: senescent, skeletal system, remodeling, matrix metalloproteinase, exercise
- Published
- 2021