1. Cellular internalization mechanism of novel Raman probes designed for plant cells
- Author
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Kousuke Tsuchiya, Takanori Iino, Keiji Numata, Yutaka Kodama, Takuya Asai, Yasuyuki Ozeki, Keiko Midorikawa, Yu Miyagi, and Simon Sau Yin Law
- Subjects
media_common.quotation_subject ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,symbols.namesake ,Internalization ,Cytotoxicity ,Molecular Biology ,Diphenylacetylene ,030304 developmental biology ,media_common ,0303 health sciences ,biology ,technology, industry, and agriculture ,Penetration (firestop) ,biology.organism_classification ,Plant cell ,0104 chemical sciences ,Chemistry ,chemistry ,Chemistry (miscellaneous) ,Biophysics ,symbols ,Tetra ,Raman spectroscopy ,Ethylene glycol - Abstract
Diphenylacetylene derivatives containing different polymeric components, poly(l-lysine) (pLys) or tetra(ethylene glycol) (TEG) were designed as novel Raman imaging probes with high Raman sensitivity and low cytotoxicity in living plant cells. The pLys-conjugated probe is internalized via an endocytosis-dependent pathway, whereas TEG-conjugated probe most likely induces direct penetration into the plant cells., Diphenyl acetylene derivatives containing various polymeric components have been designed as new Raman imaging probes. These are taken up by plant cells via different pathways, and the internalization of exogenous molecules can be visualized.
- Published
- 2020
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