1. Antigenic diversity in Theileria parva populations from sympatric cattle and African buffalo analysed using long read sequencing
- Author
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Johanneke D. Hemmink, Edith Paxton, Emmanuel Sindoya, Tiziana Lembo, W. Ivan Morrison, Harriet Auty, Stephen J. Torr, Siddharth Jayaraman, Furaha Mramba, Philip G. Toye, Tito Kibona, Ian Handel, Fiona K. Allan, Robert D. Fyumagwa, and Liam J. Morrison
- Subjects
0301 basic medicine ,Theileria parva ,animal diseases ,030231 tropical medicine ,Population ,Population genetics ,Zoology ,Biology ,QH426-470 ,molecular epidemiology ,03 medical and health sciences ,0302 clinical medicine ,Genotype ,parasitic diseases ,Genetics ,East Coast fever ,education ,Genetics (clinical) ,African buffalo ,Genetic diversity ,education.field_of_study ,Molecular epidemiology ,business.industry ,food and beverages ,biology.organism_classification ,African buffalo (Syncerus caffer) ,030104 developmental biology ,cattle ,Molecular Medicine ,Livestock ,business ,geographic locations ,Syncerus caffer - Abstract
East Coast fever (ECF) in cattle is caused by the Apicomplexan protozoan parasiteTheileria parva, transmitted by the three-host tickRhipicephalus appendiculatus. The African buffalo (Syncerus caffer) is the natural host forT. parvabut does not suffer disease, whereas ECF is often fatal in cattle. The genetic relationship betweenT. parvapopulations circulating in cattle and buffalo is poorly understood, and has not been studied in sympatric buffalo and cattle. This study aimed to determine the genetic diversity ofT. parvapopulations in cattle and buffalo, in an area where livestock co-exist with buffalo adjacent to the Serengeti National Park, Tanzania. ThreeT. parvaantigens (Tp1, Tp4, and Tp16), known to be recognized by CD8+and CD4+T cells in immunized cattle, were used to characterize genetic diversity ofT. parvain cattle (n= 126) and buffalo samples (n= 22). Long read (PacBio) sequencing was used to generate full or near-full length allelic sequences. Patterns of diversity were similar across all three antigens, with allelic diversity being significantly greater in buffalo-derived parasites compared to cattle-derived (e.g., for Tp1 median cattle allele count was 9, and 81.5 for buffalo), with very few alleles shared between species (8 of 651 alleles were shared for Tp1). Most alleles were unique to buffalo with a smaller proportion unique to cattle (412 buffalo unique vs. 231 cattle-unique for Tp1). There were indications of population substructuring, with one allelic cluster of Tp1 representing alleles found in both cattle and buffalo (including the TpM reference genome allele), and another containing predominantly only alleles deriving from buffalo. These data illustrate the complex interplay betweenT. parvapopulations in buffalo and cattle, revealing the significant genetic diversity in the buffaloT. parvapopulation, the limited sharing of parasite genotypes between the host species, and highlight that a subpopulation ofT. parvais maintained by transmission within cattle. The data indicate that fuller understanding of buffaloT. parvapopulation dynamics is needed, as only a comprehensive appreciation of the population genetics ofT. parvapopulations will enable assessment of buffalo-derived infection risk in cattle, and how this may impact upon control measures such as vaccination.
- Published
- 2021