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9 results on '"Raymonde Baltenweck"'

1. Ancestral chemotypes of cultivated grapevine with resistance to Botryosphaeriaceae‐related dieback allocate metabolism towards bioactive stilbenes

Author

Vaidurya Pratap Sahi, Alessandra Maia-Grondard, Islam M. Khattab, Eva Bieler, Michael Riemann, Peter Nick, Raymonde Baltenweck, Markus Dürrenberger, Philippe Hugueney, Karlsruhe Institut für Technologie, Bot Inst, Mol Cell Biol, Karlsruhe, Allemagne (KIT), Damanhour University, Faculty of Agriculture, Department of Horticulture, Damanhour, Egypte (agya), Karlsruhe Institute of Technology (KIT), Université de Bâle, and Karlsruher Institut für Technologie (KIT)

Subjects
0106 biological sciences, 0301 basic medicine, Life sciences, biology, Physiology, Population, Viniferin, Plant Science, Fungus, 01 natural sciences, piceid chemotype, 03 medical and health sciences, chemistry.chemical_compound, Ascomycota, Germany, ddc:570, Botany, Stilbenes, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Colonization, Vitis, Vitis resistance, education, Piceid, Plant Diseases, education.field_of_study, Chemotype, Inoculation, Botryosphaeriaceae, biology.organism_classification, resveratrol-viniferin chemotype, Plant Breeding, 030104 developmental biology, chemistry, phenyl-propanoid pathway, Neofusicoccum parvum, 010606 plant biology & botany
Abstract

Grapevine trunk diseases have devastating consequences on vineyards worldwide. European wild grapevines (Vitis vinifera subs. sylvestris) from the last viable population in Germany along the Rhine river showed variable degrees of resistance against Neofusicoccum parvum (strain Bt‐67), a fungus associated with Botryosphaeriaceae‐related dieback. - Representative genotypes from different subclades of this population were mapped with respect to their ability to induce wood necrosis, as well as their defence responses in a controlled inoculation system. - The difference in colonization patterns could be confirmed by cryo‐scanning electron microscopy, while there was no relationship between vessel diameter and infection success. Resistant lines accumulated more stilbenes, that were in addition significantly partitioned to nonglycosylated viniferin trimers. By contrast, the susceptible genotypes accumulated less stilbenes with a significantly higher proportion of glycosylated piceid. - We suggest a model in which in the resistant genotypes phenylpropanoid metabolism is channelled rapidly and specifically to the bioactive stilbenes. Our study specifies a resistant chemotype against grapevines trunk diseases and paves a way to breed for resistance against grapevine Botryosphaeriaceae‐related dieback.

Published
2021

2. Functional diversification in the Nudix hydrolase gene family drives sesquiterpene biosynthesis in Rosa × wichurana

Author

Clément Dégut, Michel A. Haring, Robert C. Schuurink, Sylvie Baudino, Jean-Louis Magnard, Ahmed Abd-El-Haliem, Jean-Claude Caissard, Saretta N. Paramita, Laurence Hibrand-Saint Oyant, Pulu Sun, Aurélie Bony, Corentin Conart, Fabrice Foucher, Tatiana Thouroude, Julien Jeauffre, Carine Tisné, Stéphane Réty, Raymonde Baltenweck, Philippe Hugueney, Jordan Marie-Magdelaine, Plant Physiology (SILS, FNWI), Swammerdam Institute for Life Sciences (SILS), University of Amsterdam [Amsterdam] (UvA), Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (LBVPAM), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Santé de la vigne et qualité du vin (SVQV), Université de Strasbourg (UNISTRA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Region Auvergne-Rhone-Alpes, CNRS GDR MediatEC: 3658, French National Research Agency (ANR)., Université Jean Monnet [Saint-Étienne] (UJM), Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université d'Angers (UA), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, CNRS GDR MediatEC : 3658., ANR: ANR, Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (BVPAM), Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and ANR-16-CE20-0024,ROSASCENT,Biosynthèse des terpènes du parfum chez la rose(2016)

Subjects
0106 biological sciences, 0301 basic medicine, Nudix structure, Quantitative Trait Loci, Heterologous, Plant Science, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Quantitative trait locus, Genes, Plant, Rosa, 01 natural sciences, Nudix hydrolase, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, 03 medical and health sciences, chemistry.chemical_compound, quantitative trait locus, Gene expression, Genetics, Gene family, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, volatile compounds, Pyrophosphatases, [SDV.SA.HORT]Life Sciences [q-bio]/Agricultural sciences/Horticulture, Gene, Phylogeny, Plant Proteins, rose scent, Cell Biology, Farnesol, 030104 developmental biology, chemistry, Biochemistry, Genetic marker, Sequence Alignment, Sesquiterpenes, Geraniol, 010606 plant biology & botany
Abstract

Roses use a non-canonical pathway involving a Nudix hydrolase, RhNUDX1, to synthesize their monoterpenes, especially geraniol. Here we report the characterization of another expressed NUDX1 gene from the rose cultivar Rosa x wichurana, RwNUDX1-2. In order to study the function of the RwNUDX1-2 protein, we analyzed the volatile profiles of an F1 progeny generated by crossing R. chinensis cv. 'Old Blush' with R. x wichurana. A correlation test of the volatilomes with gene expression data revealed that RwNUDX1-2 is involved in the biosynthesis of a group of sesquiterpenoids, especially E,E-farnesol, in addition to other sesquiterpenes. In vitro enzyme assays and heterologous in planta functional characterization of the RwNUDX1-2 gene corroborated this result. A quantitative trait locus (QTL) analysis was performed using the data of E,E-farnesol contents in the progeny and a genetic map was constructed based on gene markers. The RwNUDX1-2 gene co-localized with the QTL for E,E-farnesol content, thereby confirming its function in sesquiterpenoid biosynthesis in R. x wichurana. Finally, in order to understand the structural bases for the substrate specificity of rose NUDX proteins, the RhNUDX1 protein was crystallized, and its structure was refined to 1.7 Å. By molecular modeling of different rose NUDX1 protein complexes with their respective substrates, a structural basis for substrate discrimination by rose NUDX1 proteins is proposed.

Published
2020

3. Carrot resistance against Alternaria leaf blight: potential involvement of terpenes and flavonoids

Author

M.-H. Bouvet Merlet, Latifa Hamama, Raymonde Baltenweck, S.-A. Moussa, David Halter, A. Suel, V. Le Clerc, Patricia Claudel, Philippe Hugueney, Claude Koutouan, S. Huet, Mathilde Briard, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Santé de la vigne et qualité du vin (SVQV), and Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA)

Subjects
0106 biological sciences, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 0303 health sciences, biology, Resistance (ecology), specialized metabolites, fungi, [SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy, food and beverages, Alternaria dau, Horticulture, Alternaria, biology.organism_classification, 01 natural sciences, plant resistance, Daucus carota, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Terpene, 03 medical and health sciences, Blight, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience; Alternaria leaf blight (ALB), caused by the fungus Alternaria dauci (A. dauci), is the most damaging foliar disease affecting carrot (Daucus carota subsp. sativus). Some evidences support the potential involvement of specialized metabolites such as falcarindiol (Lecomte et al., 2012) in the resistance against ALB. In our study, we investigated the specialized metabolite composition of carrot leaf in different environments (fields and tunnel) following A. dauci infection, by comparing four carrot genotypes with different resistance levels. We identified discriminating specialized metabolites belonging to the terpene and flavonoid families, whose differential accumulation levels among genotypes correlated with their resistance. Then, we performed a bulk segregant analysis on F3 progenies to investigate the hypothesis that the discriminating terpenes and flavonoids could be linked to resistance. This study shed light on specific terpenes and flavonoids as good candidates for resistance to ALB.

Published
2019

4. Arsenite response inCoccomyxasp. Carn explored by transcriptomic and non-targeted metabolomic approaches

Author

Philippe N. Bertin, Philippe Hugueney, Olivier Bouchez, Frédéric Plewniak, Corinne Casiot, Sandrine Koechler, Florence Arsène-Ploetze, Hermann J. Heipieper, Raymonde Baltenweck, and David Halter

Subjects
0301 basic medicine, Genetics, Euglena gracilis, biology, ved/biology, 030106 microbiology, ved/biology.organism_classification_rank.species, Arsenate, chemistry.chemical_element, Glutathione, medicine.disease_cause, biology.organism_classification, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Metabolomics, chemistry, Biochemistry, medicine, Coccomyxa, Ecology, Evolution, Behavior and Systematics, Arsenic, Oxidative stress, Arsenite
Abstract

Arsenic is a toxic metalloid known to generate an important oxidative stress in cells. In the present study, we focused our attention on an alga related to the genus Coccomyxa, exhibiting an extraordinary capacity to resist high concentrations of arsenite and arsenate. The integrated analysis of high-throughput transcriptomic data and non-targeted metabolomic approaches highlighted multiple levels of protection against arsenite. Indeed, Coccomyxa sp. Carn induced a set of transporters potentially preventing the accumulation of this metalloid in the cells and presented a distinct arsenic metabolism in comparison to another species more sensitive to that compound, i.e. Euglena gracilis, especially in regard to arsenic methylation. Interestingly, Coccomyxa sp. Carn was characterized by a remarkable accumulation of the strong antioxidant glutathione (GSH). Such observation could explain the apparent low oxidative stress in the intracellular compartment, as suggested by the transcriptomic analysis. In particular, the high amount of GSH in the cell could play an important role for the tolerance to arsenate, as suggested by its partial oxidation into oxidized glutathione in presence of this metalloid. Our results therefore reveal that this alga has acquired multiple and original defence mechanisms allowing the colonization of extreme ecosystems such as acid mine drainages.

Published
2016

5. Link between carrot leaf secondary metabolites and resistance to Alternaria dauci

Author

Raymonde Baltenweck, Patricia Claudel, David Halter, A. Suel, Mathilde Briard, Valérie Le Clerc, Marie-Hélène Bouvet Merlet, Claude Koutouan, S. Huet, Latifa Hamama, Philippe Hugueney, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ministere de l'Enseignement Superieur et de la Recherche (France), French Region Pays de la Loire, European Regional Development Fund, Angers Loire Metropole, and Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
approche métabolomique, 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Secondary Metabolism, lcsh:Medicine, 01 natural sciences, Terpene, lcsh:Science, 2. Zero hunger, Vegetal Biology, Multidisciplinary, biology, Microbiology and Parasitology, Alternaria, traitement antifongique, food and beverages, daucus carota, Microbiologie et Parasitologie, Agricultural sciences, Horticulture, Metabolome, composante de la résistance, agent pathogène, Genotype, Phytopathology and phytopharmacy, composition phénologique, huiles essentielles, Fungus, composant volatil, Article, 03 medical and health sciences, Metabolomics, Alternaria dauci, Blight, Plant Diseases, identification biochimique, bacteriose, lcsh:R, biology.organism_classification, Phytopathologie et phytopharmacie, Plant Leaves, 030104 developmental biology, lcsh:Q, Biologie végétale, Sciences agricoles, 010606 plant biology & botany, Daucus carota
Abstract

Alternaria Leaf Blight (ALB), caused by the fungus Alternaria dauci, is the most damaging foliar disease affecting carrots (Daucus carota). In order to identify compounds potentially linked to the resistance to A. dauci, we have used a combination of targeted and non-targeted metabolomics to compare the leaf metabolome of four carrot genotypes with different resistance levels. Targeted analyses were focused on terpene volatiles, while total leaf methanolic extracts were subjected to non-targeted analyses using liquid chromatography couple to high-resolution mass spectrometry. Differences in the accumulation of major metabolites were highlighted among genotypes and some of these metabolites were identified as potentially involved in resistance or susceptibility. A bulk segregant analysis on F3 progenies obtained from a cross between one of the resistant genotypes and a susceptible one, confirmed or refuted the hypothesis that the metabolites differentially accumulated by these two parents could be linked to resistance.

Published
2018

6. A grapevine cytochrome P450 generates the precursor of wine lactone, a key odorant in wine

Author

Lucie Kriegshauser, Laurence Miesch, Philippe Hugueney, Andrea Ilg, Florian Lauvoisard, Danièle Werck-Reichhart, Eric Duchêne, Tina Ilc, Raymonde Baltenweck, David Halter, Nicolas Navrot, Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), Université de Strasbourg (UNISTRA), Santé de la vigne et qualité du vin (SVQV), Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA), Agence Nationale de la Recherche [ANR-13-BSV6-0010], European Union under REA [289217], Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut de Chimie de Strasbourg, Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC), Laboratoire de Chimie Quantique, Centre National de la Recherche Scientifique (CNRS), and Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, Physiology, cytochrome P450, Acyclic Monoterpenes, [SDV]Life Sciences [q-bio], Quantitative Trait Loci, Aroma of wine, Wine, Plant Science, wine aroma, wine lactone, Genes, Plant, 01 natural sciences, quantitative trait locus (QTL), Lactones, 03 medical and health sciences, chemistry.chemical_compound, Cytochrome P-450 Enzyme System, Linalool, Gene Expression Regulation, Plant, Tobacco, Botany, Malolactic fermentation, Aroma compound, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, Food science, Aroma, Plant Proteins, biology, Wine lactone, biology.organism_classification, grapevine, Plant Leaves, Yeast in winemaking, 030104 developmental biology, chemistry, Fruit, Odorants, Monoterpenes, 010606 plant biology & botany
Abstract

Monoterpenes are important constituents of the aromas of food and beverages, including wine. Among monoterpenes in wines, wine lactone has the most potent odor. It was proposed to form via acid-catalyzed cyclization of (E)-8-carboxylinalool during wine maturation. It only reaches very low concentrations in wine but its extremely low odor detection threshold makes it an important aroma compound. Using LC-MS/MS, we show here that the (E)-8-carboxylinalool content in wines correlates with their wine lactone content and estimate the kinetic constant for the very slow formation of wine lactone from (E)-8-carboxylinalool. We show that (E)-8-carboxylinalool is accumulated as a glycoside in grape (Vitis vinifera) berries and that one of the cytochrome P450 enzymes most highly expressed in maturing berries, CYP76F14, efficiently oxidizes linalool to (E)-8-carboxylinalool. Our analysis of (E)-8-carboxylinalool in Riesling × Gewurztraminer grapevine progeny established that the CYP76F14 gene co-locates with a quantitative trait locus for (E)-8-carboxylinalool content in grape berries. Our data support the role of CYP76F14 as the major (E)-8-carboxylinalool synthase in grape berries and the role of (E)-8-carboxylinalool as a precursor to wine lactone in wine, providing new insights into wine and grape aroma metabolism, and new methods for food and aroma research and production.

Published
2017

7. Biosynthesis of monoterpene scent compounds in roses

Author

Stéphanie Huguet, Julien Jeauffre, Sophie Meyer, Raymonde Baltenweck, Sylvie Baudino, Michel Rohmer, Annick Dubois, Aymeric Roccia, Mohammed Bendahmane, Fabrice Foucher, Romain Hecquet, Patricia Claudel, Laurence Hibrand-Saint Oyant, Philippe Hugueney, Jean-Claude Caissard, Florence Nicolè, Pulu Sun, Frédéric Jullien, Jean-Louis Magnard, Olivier Raymond, Philippe Vergne, Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (LBVPAM), Université Jean Monnet [Saint-Étienne] (UJM), Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), Institut Le Bel, Region Rhone-Alpes, CNRS [GDR 2827], ENS Lyon, INRA, Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales (BVPAM), Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Laboratoire BVpam, Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)

Subjects
0106 biological sciences, Rose, pyrophosphatase, Monoterpene, [SDV]Life Sciences [q-bio], Biology, plastide, Biosynthesis, 01 natural sciences, Nudix hydrolase, Terpene, 03 medical and health sciences, chemistry.chemical_compound, Botany, donnée de séquence moléculaire, Cultivar, odeur, 2-phenylethanol, Geranyl diphosphate, biosynthesis, rose, monoterpene, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Multidisciplinary, fungi, food and beverages, Metabolic pathway, Enzyme, Biochemistry, chemistry, transcriptome, Geraniol, 010606 plant biology & botany
Abstract

Stop to smell the roses Some roses smell beautiful, yet others only look beautiful. Magnard et al. leveraged this distinction to study the biosynthesis of geraniol, a monoterpene alcohol in rose scent (see the Perspective by Tholl and Gershenzon). Enzymes known for geraniol synthesis in other plants, such as basil, did not seem to provide that function for roses. Instead, a diphosphohydrolase, which functions in the cytoplasm of cells in rose petals, generates the geraniol emitted by fragrant roses. Identification of the enzyme and its gene enables marker-assisted breeding to put the perfume back into beauty. Science , this issue p. 81 ; see also p. 28

Published
2015

8. Gene Coexpression Analysis Reveals Complex Metabolism of the Monoterpene Alcohol Linalool in Arabidopsis FlowersW

Author

René Höfer, Francel W.A. Verstappen, Raphaël Lugan, Francis Karst, Michel Miesch, Laurence Miesch, Marc J. C. Fischer, Franziska Beran, Danièle Werck-Reichhart, Patricia Claudel, Jonathan Gershenzon, Jean-François Ginglinger, Raymonde Baltenweck, Tobias G. Köllner, Harro J. Bouwmeester, Pascaline Ullmann, Bernd Schneider, Benoît Boachon, Christian Paetz, Jérôme Mutterer, Jürgen Ehlting, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Max Planck Institute for Chemical Ecology, Max-Planck-Gesellschaft, Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Laboratory of Plant Physiology, Wageningen University and Research [Wageningen] (WUR), Department of Biology, Centre for Forest Biology, University of Victoria [Canada] (UVIC), METAMAP [ANR-07-BLAN-0359 - CSD 7], European Community, Centre National de la Recherche Scientifique, and Region Alsace for a scholarship

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], Monoterpene, Arabidopsis, Nicotiana benthamiana, Plant Science, Beta-glucuronidase, 01 natural sciences, avocado persea-americana, Terpene, chemistry.chemical_compound, Cytochrome P-450 Enzyme System, Linalool, Gene Expression Regulation, Plant, Arabidopsis thaliana, Laboratorium voor Plantenfysiologie, Intramolecular Lyases, mint mentha, ComputingMilieux_MISCELLANEOUS, Research Articles, 0303 health sciences, biology, cytochrome-p450 limonene hydroxylases, EPS-3, food and beverages, Plants, Genetically Modified, Biochemistry, terpene synthases, saccharomyces-cerevisiae, Laboratory of Plant Physiology, Acyclic Monoterpenes, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Flowers, Saccharomyces cerevisiae, essential oil, 03 medical and health sciences, Tobacco, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, 030304 developmental biology, floral scent, Arabidopsis Proteins, fungi, beta-glucuronidase, endoplasmic-reticulum, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, biology.organism_classification, chemistry, Mutation, Monoterpenes, functional expression, Petal, 010606 plant biology & botany
Abstract

The cytochrome P450 family encompasses the largest family of enzymes in plant metabolism, and the functions of many of its members in Arabidopsis thaliana are still unknown. Gene coexpression analysis pointed to two P450s that were coexpressed with two monoterpene synthases in flowers and were thus predicted to be involved in monoterpenoid metabolism. We show that all four selected genes, the two terpene synthases (TPS10 and TPS14) and the two cytochrome P450s (CYP71B31 and CYP76C3), are simultaneously expressed at anthesis, mainly in upper anther filaments and in petals. Upon transient expression in Nicotiana benthamiana, the TPS enzymes colocalize in vesicular structures associated with the plastid surface, whereas the P450 proteins were detected in the endoplasmic reticulum. Whether they were expressed in Saccharomyces cerevisiae or in N. benthamiana, the TPS enzymes formed two different enantiomers of linalool: (−)-(R)-linalool for TPS10 and (+)-(S)-linalool for TPS14. Both P450 enzymes metabolize the two linalool enantiomers to form different but overlapping sets of hydroxylated or epoxidized products. These oxygenated products are not emitted into the floral headspace, but accumulate in floral tissues as further converted or conjugated metabolites. This work reveals complex linalool metabolism in Arabidopsis flowers, the ecological role of which remains to be determined.

Published
2013

9. Genetic analysis of the biosynthesis of 2-methoxy-3-isobutylpyrazine, a major grape-derived aroma compound impacting wine quality

Author

Andrea Ilg, Philippe Hugueney, Eric Duchêne, Céline Léon, Eric Gomès, Stéphane Réty, Sandra Vanbrabant, Louis Bordenave, Claudine Trossat-Magnin, Stéphane Decroocq, Patricia Claudel, Tao Ye, Philippe Darriet, Sabine Guillaumie, Raymonde Baltenweck-Guyot, Céline Keime, Serge Delrot, Maxime Brette, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Centre National de la Recherche Scientifique (CNRS), Oenologie (UMRO), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
0106 biological sciences, [SDE] Environmental Sciences, qualité du vin, Physiology, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], Plant Science, Biology, biosynthèse, 01 natural sciences, Genetic analysis, composé aromatique, baie de raisin, 03 medical and health sciences, chemistry.chemical_compound, Methoxypyrazines, méthoxylation, vitis vinifera, Biosynthesis, Botany, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Genetics, Aroma compound, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Food science, Differential expression, Vitis vinifera, 030304 developmental biology, Wine, 0303 health sciences, qtl, food and beverages, vinification, thiol volatil, [SDV.IDA] Life Sciences [q-bio]/Food engineering, [SDV] Life Sciences [q-bio], chemistry, [SDE]Environmental Sciences, spectrométrie, 2-methoxy-3-isobutylpyrazine, analyse génétique, expression des gènes, 010606 plant biology & botany
Abstract

L'article original est publié par The American Society of Plant Biologists; International audience; Methoxypyrazines (MPs) are strongly odorant volatile molecules with vegetable-like fragrances that are widespread in plants. Some grapevine (Vitis vinifera) varieties accumulate significant amounts of MPs, including 2-methoxy-3-isobutylpyrazine (IBMP), which is the major MP in grape berries. MPs are of particular importance in white Sauvignon Blanc wines. The typicality of these wines relies on a fine balance between the pea pod, capsicum character ofMPs and the passion fruit/grapefruit character due to volatile thiols. Although MPs play a crucial role in Sauvignon varietal aromas, excessive concentrations of these powerful odorants alter wine quality and reduce consumer acceptance, particularly in red wines. The last step of IBMP biosynthesis has been proposed to involve the methoxylation of the nonvolatile precursor 2-hydroxy-3-isobutylpyrazine to give rise to the highly volatile IBMP. In this work, we have used a quantitative trait loci approach to investigate the genetic bases of IBMP biosynthesis. This has led to the identification of two previously uncharacterized S-adenosyl-methionine-dependent O-methyltransferase genes, termed VvOMT3 and VvOMT4. Functional characterization of these two O-methyltransferases showed that the VvOMT3 protein was highly specific and efficient for 2-hydroxy-3-isobutylpyrazine methylation. Based on its differential expression in high-and low-MP-producing grapevine varieties, we propose that VvOMT3 is a key gene for IBMP biosynthesis in grapevine.

Published
2013

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