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1. Biomechanical tensile behavior of human Wharton’s jelly

Author

Johan Sergheraert, Adrien Baldit, Cédric Mauprivez, Marie Dubus, Halima Kerdjoudj, Rachid Rahouadj, Laboratoire d'Etude des Microstructures et de Mécanique des Matériaux (LEM3), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), Ecole Nationale d'Ingénieurs de Metz (ENIM), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), and Centre Hospitalier Universitaire de Reims (CHU Reims) more...

Subjects
Computer science, Cyclic tensile characterization, [SDV]Life Sciences [q-bio], Biomedical Engineering, Failure, Umbilical Cord, Biomaterials, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Biological property, Wharton's jelly, Ultimate tensile strength, Humans, Biomechanics, Wharton Jelly, Warthon’s jelly, Cells, Cultured, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Viscosity, Hyperelasticity, Cell Differentiation, Mesenchymal Stem Cells, Numerical models, Predictive simulation, Tensile behavior, Mechanics of Materials, Biomedical engineering
Abstract

International audience; Wharton's jelly (WJ) is a mucous connective tissue of the umbilical cord. It shows high healing capabilities, mainly attributed to the chemical composition and to the presence of stem cells, growth factors and peptides. Although WJ biological properties are well documented in vitro and in vivo, there is still a lack of mechanical data on this tissue, which is paramount for its use as a biomaterial for medical applications. In this study, mechanical responses of ten WJ samples within close physiological conditions were registered undergoing quasi static cyclic tensile tests followed by a load up to failure. This protocol aimed on one hand to provide biomechanical data to feed predictive numerical models and on the other hand increase WJ knowledge in view of its potential use in biomedical field. In spite of the WJ harvest, the resulting viscous nonlinear elastic response obtained is fully in tune with the literature confirming the database quality. A side of the knowledge improvement on WJ mechanical response, this paper provides accurate data that will enhance predictive simulation work such as finite element analysis. The mechanical step-through brought by the analytical nonlinear characterization over cyclic and ultimate loads is to predict WJ behavior. Actually, principal component analysis highlighted its quality while pointing out indicators, such as failure or hydration criteria, as well as models' limitations. more...

Published
2022
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2. Bone marrow mesenchymal stem cells offer an immune-privileged niche to Cutibacterium acnes in case of implant-associated osteomyelitis

Author

Fany Reffuveille, Fabienne Quilès, Marie Dubus, Halima Kerdjoudj, S. Papa, Cédric Mauprivez, Julie Chevrier, R. Siboni, X. Ohl, J. Varin, Sophie C. Gangloff, Grégory Francius, Sandra Audonnet, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Plateau Technique de Cytométrie en Flux (URCACyt), Pôle Odontologie [CHU Reims], Centre Hospitalier Universitaire de Reims (CHU Reims), and Colin, Marius more...

Subjects
Implant-associated infection, [SDV]Life Sciences [q-bio], Biomedical Engineering, Bone Marrow Cells, Bone healing, Biology, Biochemistry, Microbiology, Biomaterials, 03 medical and health sciences, Immune system, medicine, Humans, Macrophage, Secretion, Propionibacterium acnes, Molecular Biology, 030304 developmental biology, 0303 health sciences, 030306 microbiology, Intracellular parasite, Osteomyelitis, Mesenchymal stem cell, Biofilm, Prostheses and Implants, General Medicine, medicine.disease, 3. Good health, [SDV] Life Sciences [q-bio], Biofilms, Bone repair, Mesenchymal stem cells, Bacteria/ stem cells interactions, Cutibacterium acnes, Biotechnology
Abstract

Considered as some of the most devastating complications, Cutibacterium acnes (C. acnes)-related osteomyelitis are among the hardest infections to diagnose and treat. Mesenchymal stem cells (MSCs) secrete number of immunomodulatory and antimicrobial soluble factors, making them an attractive treatment for bacterial infection. In this study, we examined MSCs/C. acnes interaction and analyzed the subsequent MSCs and bacteria's behaviors. Human bone marrow-derived MSCs were infected by C. acnes clinical strain harvested from non-infected bone site. Following 3 h of interaction, around 4% of bacteria were found in the intracellular compartment. Infected MSCs increased the secretion of prostaglandin E2 and indolamine 2,3 dioxygenase immunomodulatory mediators. Viable intracellular bacteria analyzed by infrared spectroscopy and atomic force microscopy revealed deep modifications in the wall features. In comparison with unchallenged bacteria, the viable intracellular bacteria showed (i) an increase in biofilm formation on orthopaedical-based materials, (ii) an increase in the invasiveness of osteoblasts and (iii) persistence in macrophage, suggesting the acquisition of virulence factors. Overall, these results showed a direct impact of C. acnes on bone marrow-derived MSCs, suggesting that blocking the C. acnes/MSCs interactions may represent an important new approach to manage chronic osteomyelitis infections. Statement of significance The interaction of bone commensal C. acnes with bone marrow mesenchymal stem cells induces modifications in C. acnes wall characteristics. These bacteria increased (i) the biofilm formation on orthopaedical-based materials, (ii) the invasiveness of bone forming cells and (iii) the resistance to macrophage clearance through the modification of the wall nano-features and/or the increase in catalase production. more...

Published
2022
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3. Haemostatic sponges as an alternative to granular bone substitute for sinus lifts

Author

S. Okley, A. Baldit, P. Prada, Rachid Rahouadj, Cédric Mauprivez, Marie Dubus, Frédéric Velard, Halima Kerdjoudj, Laboratoire d'Etude des Microstructures et de Mécanique des Matériaux (LEM3), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), Ecole Nationale d'Ingénieurs de Metz (ENIM), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV) more...

Subjects
Bone substitute, microstructure, 0206 medical engineering, Biomedical Engineering, Sinus lift, Bioengineering, 02 engineering and technology, gelatin sponge, mechanical properties, [SPI.MECA.SOLID]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Solid mechanics [physics.class-ph], 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, [SPI.MECA.MEMA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanics of materials [physics.class-ph], otorhinolaryngologic diseases, medicine, Sinus (anatomy), Dental alveolus, Gelatin sponge, business.industry, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], 030229 sport sciences, General Medicine, Anatomy, 020601 biomedical engineering, [SPI.MECA.GEME]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanical engineering [physics.class-ph], Computer Science Applications, Human-Computer Interaction, stomatognathic diseases, medicine.anatomical_structure, collagen sponge, Collagen sponge, [SPI.MECA.STRU]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Structural mechanics [physics.class-ph], business, Posterior maxilla
Abstract

International audience; Insufficient residual alveolar bone height is a common obstacle for the placement of dental implants in the posterior maxilla. Sinus lifting procedure involves the creation of a sub-sinus membrane pocket and the grafting with granular bone substitutes. Perforation of the sinus membrane is the most common complication with a large incidence rate (Testori et al. 2019). Graft material leak, through the perforation, results in a dental implant failure and/or postoperative sinusitis, requiring a complete reviewing of the maxillary sinus cavity and the removal of the remaining bone graft. The high perforation rate has led to develop new options involving 3 D biodegradable scaffolds instead of using granular bone substitutes. Case reports and case series have pointed out successful new bone formation after sinus membrane elevation using reoxidised cellulose (Gray et al. 2001), venous blood clot (Moon et al. 2011) and gelatin sponge (Sohn et al. 2010) as space maintainers. The main limitation of this promising technique was that the sub membrane space volume was limited and hardly predictable. The aim of this study was to evaluate the mechanical behaviour and volume kinematics of several haemostatic sponges to find out the best haemostatic medical device as space maintainers for new bone formation in sinus lift surgery. This study has been focused only on two devices: Gelita-spon® and Hemocollagene®. It aims to compare substitutes respectively made of porcine gelatine and bovine collagen. more...

Published
2020
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4. Mechanical behaviour of a membrane made of human umbilical cord for dental bone regenerative medicine

Author

Marie Dubus, Cédric Mauprivez, L. Scomazzon, Halima Kerdjoudj, Johan Sergheraert, R. Rahouadj, A. Baldit, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Laboratoire d'Etude des Microstructures et de Mécanique des Matériaux (LEM3), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), and Ecole Nationale d'Ingénieurs de Metz (ENIM) more...

Subjects
0206 medical engineering, Biomedical Engineering, Dentistry, Mechanical properties, Bioengineering, 02 engineering and technology, [SPI.MECA.SOLID]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Solid mechanics [physics.class-ph], Umbilical cord, Regenerative medicine, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Periodontal disease, Bio-Gide®, [SPI.MECA.MEMA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanics of materials [physics.class-ph], medicine, hyperelasticity, Bone regeneration, business.industry, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], 030229 sport sciences, General Medicine, 020601 biomedical engineering, [SPI.MECA.GEME]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanical engineering [physics.class-ph], Computer Science Applications, Human-Computer Interaction, stomatognathic diseases, medicine.anatomical_structure, guided bone regeneration, [SPI.MECA.STRU]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Structural mechanics [physics.class-ph], human umbilical cord, business
Abstract

International audience; Guided bone regeneration (GBR) is one of the most attractive techniques for restoring oral bone defects following tooth extraction or periodontal disease. To ensure the stability of a dental implant, the GBR technique relies on the use of an occlusive membrane, which is positioned as a barrier over the bone defect, providing space maintenance required for bone growth and preventing the ingrowth of fibrous tissue into the bone defect (Elgali et al. 2017). This membrane has to meet criteria guidelines in terms of mechanical properties, as the membrane has to allow a good handling for dental surgeons, without collapsing into the bone defect. Healthy perinatal tissues are promising biomaterials because they are inexpensive, and universally available (Ferguson and Dodson 2009). Among these tissues, the human umbilical cord, mainly composed of collagen fibres and glycosaminoglycan, especially hyaluronic acid and chondroitin sulphate, is expected to offer outstanding opportunities for tissue engineering by serving as a suitable biocompatible membrane for GBR.In this study, a novel membrane derived from the human umbilical cord (UC-membrane) was successfully developed following tissue stripping and freeze-drying processes. Ice crystal formation following tissue freezing is known to induce pore formation within the tissue, hampering their mechanical properties. Herein, the mechanical behaviour of UC-membrane was determined and compared to the Bio-Gide® membrane (gold standard membrane) more...

Published
2020
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5. Biomimetic Hierarchical Structuring of PLA by Ultra-Short Laser Pulses for Processing of Tissue Engineered Matrices: Study of Cellular and Antibacterial Behavior

Author

Halima Kerdjoudj, L. Angelova, A. Daskalova, Ivan Buchvarov, Emil Filipov, Julie Chevrier, Dante Aceti, Rosica Mincheva, Xavier Carrete, A. Trifonov, Marie Dubus, Institute of Electronics [Sofia], Bulgarian Academy of Sciences (BAS), Laboratory of Polymeric and Composite Materials, Université de Mons-Hainaut (LPCM), Université de Mons-Hainaut, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), and Faculty of Physics, Sofia University saint Kliment Ohridski more...

Subjects
Materials science, Polymers and Plastics, Scanning electron microscope, [SDV]Life Sciences [q-bio], FOS: Physical sciences, Organic chemistry, 02 engineering and technology, Surface finish, Article, 03 medical and health sciences, QD241-441, X-ray photoelectron spectroscopy, temporal scaffolds, Surface roughness, Physics - Biological Physics, Fourier transform infrared spectroscopy, PLA texturing, 030304 developmental biology, 0303 health sciences, cell matrices, General Chemistry, respiratory system, 021001 nanoscience & nanotechnology, Microstructure, Chemical engineering, Biological Physics (physics.bio-ph), ultra-short functionalization of cell matrices, tissue engineering, Surface modification, Fs bioscaffolds structuring, Wetting, 0210 nano-technology
Abstract

The influence of ultra-short laser modification on the surface morphology and possible chemical alteration of poly-lactic acid (PLA) matrix in respect to the optimization of cellular and antibacterial behavior were investigated in this study. Scanning electron microscopy (SEM) morphological examination of the processed PLA surface showed the formation of diverse hierarchical surface microstructures, generated by irradiation with a range of laser fluences (F) and scanning velocities (V) values. By controlling the laser parameters, diverse surface roughness can be achieved, thus influencing cellular dynamics. This surface feedback can be applied to finely tune and control diverse biomaterial surface properties like wettability, reflectivity, and biomimetics. The triggering of thermal effects, leading to the ejection of material with subsequent solidification and formation of raised rims and 3D-like hollow structures along the processed zones, demonstrated a direct correlation to the wettability of the PLA. A transition from superhydrophobic (θ &gt, 150°) to super hydrophilic (θ &lt, 20°) surfaces can be achieved by the creation of grooves with V = 0.6 mm/s, F = 1.7 J/cm2. The achieved hierarchical architecture affected morphology and thickness of the processed samples which were linked to the nature of ultra-short laser-material interaction effects, namely the precipitation of temperature distribution during material processing can be strongly minimized with ultrashort pulses leading to non-thermal and spatially localized effects that can facilitate volume ablation without collateral thermal damage The obtained modification zones were analyzed employing Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray analysis (EDX), and optical profilometer. The modification of the PLA surface resulted in an increased roughness value for treatment with lower velocities (V = 0.6 mm/s). Thus, the substrate gains a 3D-like architecture and forms a natural matrix by microprocessing with V = 0.6 mm/s, F = 1.7 J/cm2, and V = 3.8 mm/s, F = 0.8 J/cm2. The tests performed with Mesenchymal stem cells (MSCs) demonstrated that the ultra-short laser surface modification altered the cell orientation and promoted cell growth. The topographical design was tested also for the effectiveness of bacterial attachment concerning chosen parameters for the creation of an array with defined geometrical patterns. more...

Published
2021
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6. Biopolymers-calcium phosphate antibacterial coating reduces the pathogenicity of internalized bacteria by mesenchymal stromal cells

Author

Halima Alem, Halima Kerdjoudj, Marie Dubus, Fany Reffuveille, Fouzia Boulmedais, Hassan Rammal, Jennifer Varin-Simon, Pierre Prada, Cédric Mauprivez, Loïc Scomazzon, Boulmedais, Fouzia, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), and Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) more...

Subjects
0301 basic medicine, Calcium Phosphates, Staphylococcus aureus, Stromal cell, media_common.quotation_subject, [SDV]Life Sciences [q-bio], education, Biomedical Engineering, 02 engineering and technology, medicine.disease_cause, Microbiology, 03 medical and health sciences, Biopolymers, [CHIM] Chemical Sciences, medicine, [CHIM]Chemical Sciences, General Materials Science, Internalization, ComputingMilieux_MISCELLANEOUS, media_common, biology, Virulence, Chemistry, Mesenchymal stem cell, Biofilm, Mesenchymal Stem Cells, Bone prosthesis, 021001 nanoscience & nanotechnology, biology.organism_classification, Anti-Bacterial Agents, [SDV] Life Sciences [q-bio], 030104 developmental biology, medicine.anatomical_structure, Bone marrow, 0210 nano-technology, Bacteria
Abstract

A multifunctional material system that kills bacteria and drives bone healing is urgently sought to improve bone prosthesis. Herein, the osteoinductive coating made of calcium phosphate/chitosan/hyaluronic acid, named Hybrid, was proposed as an antibacterial substrate for stromal cell adhesion. This Hybrid coating possesses a contact-killing effect reducing by 90% the viability of Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) strains after 48 h of contact. In addition to the production of immunomodulatory mediators, Wharton's jelly (WJ-SCs), dental pulp (DPSCs) and bone marrow (BM-MSCs) derived stromal cells were able to release antibacterial and antibiofilm agents effective against S. aureus and P. aeruginosa strains, respectively. Studying the effect of the Hybrid coating on the internalization of S. aureus by the stromal cells, in acute-mimicking bone infection, highlighted an increase in the bacteria internalization by DPSCs and BM-MSCs when cultured on the Hybrid coating versus uncoated glass. Despite the internalization, Hybrid coating showed a beneficial effect by reducing the pathogenicity of the internalized bacteria. The formation of biofilm was reduced by at least 50% in comparison to internalized bacteria by stromal cells on uncoated glass. This work opens the route for the development of innovative antibacterial coatings by taking into account the internalization of bacteria by stromal cells. more...

Published
2020
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7. Bioinspired Nanofeatured Substrates: Suitable Environment for Bone Regeneration

Author

Fany Reffuveille, Fouzia Boulmedais, Christine Terryn, Fabienne Quilès, Grégory Francius, Halima Alem, Hassan Rammal, Halima Kerdjoudj, Dominique Laurent-Maquin, Pierre Schaaf, Sophie C. Gangloff, Marie Dubus, L. Aubert, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Plateforme en Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université de Strasbourg (UNISTRA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Hocquel, Romain, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) more...

Subjects
Vascular Endothelial Growth Factor A, 0301 basic medicine, Bone Regeneration, Materials science, Surface Properties, [SDV]Life Sciences [q-bio], Aucun, 02 engineering and technology, Bone healing, engineering.material, Bone tissue, 03 medical and health sciences, Mechanobiology, Coated Materials, Biocompatible, Osteoprotegerin, Osteogenesis, [CHIM] Chemical Sciences, medicine, [CHIM]Chemical Sciences, General Materials Science, Bone regeneration, ComputingMilieux_MISCELLANEOUS, Osteoblasts, Regeneration (biology), 021001 nanoscience & nanotechnology, Nanostructures, [SDV] Life Sciences [q-bio], Durapatite, 030104 developmental biology, medicine.anatomical_structure, engineering, Biophysics, Biopolymer, Stem cell, 0210 nano-technology
Abstract

Bone mimicking coatings provide a complex microenvironment in which material, through its inherent properties (such as nanostructure and composition), affects the commitment of stem cells into bone lineage and the production of bone tissue regulating factors required for bone healing and regeneration. Herein, a bioactive mineral/biopolymer composite made of calcium phosphate/chitosan and hyaluronic acid (CaP-CHI-HA) was elaborated using a versatile simultaneous spray coating of interacting species. The resulting CaP-CHI-HA coating was mainly constituted of bioactive, carbonated and crystalline hydroxyapatite with 277 ± 98 nm of roughness, 1 μm of thickness, and 2.3 ± 1 GPa of stiffness. After five days of culture, CaP-CHI-HA suggested a synergistic effect of intrinsic biophysical features and biopolymers on stem cell mechanobiology and nuclear organization, leading to the expression of an early osteoblast-like phenotype and the production of bone tissue regulating factors such as osteoprotegerin and vascular endothelial growth factor. More interestingly, amalgamation with biopolymers conferred to the mineral a bacterial antiadhesive property. These significant data shed light on the potential regenerative application of CaP-CHI-HA bioinspired coating in providing a suitable environment for stem cell bone regeneration and an ideal strategy to prevent implant-associated infections. journal article 2017 Apr 12 2017 03 30 imported more...

Published
2017
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8. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine

Author

Camille Boulagnon-Rombi, Marie Dubus, Roselyne Garnotel, Cédric Mauprivez, Céline Mongaret, Halima Kerdjoudj, Camille Bour, Frédéric Velard, Céline Schneider, Sophie C. Gangloff, Rachid Rahouadj, L. Aubert, Cédric Laurent, Hassan Rammal, Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Université de Reims Champagne-Ardenne (URCA), Centre Hospitalier Universitaire de Reims (CHU Reims), Hôpital universitaire Robert Debré [Reims], Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Groupe d'Étude sur les Géomatériaux et Environnements Naturels, Anthropiques et Archéologiques - EA 3795 (GEGENAA), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Maison des Sciences Humaines de Champagne-Ardenne (MSH-URCA), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Pharmacologie et Toxicologie [CHU Reims], Laboratoire d'anatomie et cytologie pathologiques [Reims], and Laurent, Cédric more...

Subjects
0301 basic medicine, [PHYS.MECA.VIBR] Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], Scaffold, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, stem cell niche, medical device, regenerative medicine, biocompatibility, paracrine activities, [PHYS.MECA.MSMECA] Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph], 02 engineering and technology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SPI.MAT] Engineering Sciences [physics]/Materials, Regenerative medicine, [SPI.MAT]Engineering Sciences [physics]/Materials, lcsh:Chemistry, [PHYS.MECA.MEMA]Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], Tissue culture, [PHYS.MECA.STRU]Physics [physics]/Mechanics [physics]/Structural mechanics [physics.class-ph], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [PHYS.MECA.SOLID]Physics [physics]/Mechanics [physics]/Solid mechanics [physics.class-ph], lcsh:QH301-705.5, Spectroscopy, ComputingMilieux_MISCELLANEOUS, [PHYS.MECA.SOLID] Physics [physics]/Mechanics [physics]/Solid mechanics [physics.class-ph], [PHYS.MECA.VIBR]Physics [physics]/Mechanics [physics]/Vibrations [physics.class-ph], General Medicine, 021001 nanoscience & nanotechnology, 3. Good health, Computer Science Applications, Cell biology, Cellular Microenvironment, Collagen, Stem cell, 0210 nano-technology, Type I collagen, Biocompatibility, Preservation, Biological, Nanotechnology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, [PHYS.MECA.MEMA] Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Humans, Physical and Theoretical Chemistry, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Molecular Biology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [PHYS.MECA.STRU] Physics [physics]/Mechanics [physics]/Structural mechanics [physics.class-ph], Organic Chemistry, Mesenchymal stem cell, [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Mesenchymal Stem Cells, [PHYS.MECA.MSMECA]Physics [physics]/Mechanics [physics]/Materials and structures in mechanics [physics.class-ph], [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, Transplantation, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999
Abstract

Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE®, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE® foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton’s jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications. more...

Published
2017
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