Search

Your search keyword '"MESH: Leukocytes, Mononuclear/metabolism"' showing total 3 results
Start Over Descriptor "MESH: Leukocytes, Mononuclear/metabolism" Topic 03 medical and health sciences
3 results on '"MESH: Leukocytes, Mononuclear/metabolism"'

1. Evaluation of anti-proliferative and anti-inflammatory activities of Pelagia noctiluca venom in Lipopolysaccharide/Interferon-γ stimulated RAW264.7 macrophages

Author

Rabiaa M. Sghaier, Yosra Ayed, Dhafer Laouini, Hassen Bacha, College of Dentistry, Taibah University, Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine of Monastir, Laboratoire de Transmission, Contrôle et Immunobiologie des Infections ( LR11IPT02 ), Institut Pasteur de Tunis-Réseau International des Instituts Pasteur ( RIIP ), Université Tunis El Manar ( UTM ), Faculté de Médecine Dentaire de Monastir [Tunisie] (FMDM), Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), and Université de Jendouba (UJ)

Subjects
Lipopolysaccharides, 0301 basic medicine, Lipopolysaccharide, MESH: Cell Survival/physiology, MESH: Lipopolysaccharides/toxicity, [SDV]Life Sciences [q-bio], Anti-Inflammatory Agents, Drug Evaluation, Preclinical, Nitric Oxide Synthase Type II, Venom, MESH: Cell Proliferation/drug effects, MESH: Leukocytes, Mononuclear/drug effects, MESH: Dose-Response Relationship, Drug, Mice, chemistry.chemical_compound, 0302 clinical medicine, MESH: Animals, MESH: Interferon-gamma/toxicity, MESH: Cnidarian Venoms/isolation & purification, Cell proliferation, ComputingMilieux_MISCELLANEOUS, MTT assay, biology, MESH: Nitric Oxide Synthase Type II/metabolism, General Medicine, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, 3. Good health, MESH: Drug Evaluation, Preclinical/methods, 030220 oncology & carcinogenesis, MESH: Leukocytes, Mononuclear/metabolism, Cell Survival, Peripheral blood mononuclear cell, MESH: Cnidarian Venoms/pharmacology, Cell Line, Nitric oxide, Microbiology, Interferon-gamma, 03 medical and health sciences, Anti-inflammatory activity, Cnidarian Venoms, Animals, Humans, MESH: Macrophages/metabolism, MESH: Nitric Oxide Synthase Type II/antagonists & inhibitors, MESH: Macrophages/drug effects, MESH: Mice, Pelagia noctiluca, Pharmacology, MESH: K562 Cells, MESH: Humans, Dose-Response Relationship, Drug, [ SDV ] Life Sciences [q-bio], Cell growth, Macrophages, MESH: Cell Proliferation/physiology, biology.organism_classification, MESH: Anti-Inflammatory Agents/isolation & purification, Molecular biology, MESH: Cell Line, 030104 developmental biology, chemistry, Cell culture, Leukocytes, Mononuclear, MESH: Anti-Inflammatory Agents/pharmacology, K562 Cells, MESH: Cell Survival/drug effects, K562 cells
Abstract

International audience; Components of Pelagia noctiluca (P. noctiluca) venom were evaluated for their anticancer and nitric Oxide (NO) inhibition activities. Three fractions, out of four, obtained by gel filtration on Sephadex G75 of P. noctiluca venom revealed an important selective anti-proliferative activity on several cell lines such as human bladder carcinoma (RT112), human glioblastoma (U87), and human myelogenous leukemia (K562) but not on mitogen-stimulated peripheral blood mononuclear cells. Interestingly, P. noctiluca components showed an important dose-dependent anti-inflammatory activity, through inhibition of NO production via transcriptional regulation of Inducible NO Synthase (iNOS), in IFN-γ/LPS stimulated RAW 264.7 macrophages. These data strongly suggest that P. noctiluca venom could be used as a natural inhibitor of cancer cell lines and a potent anti-inflammatory agent for the treatment of anti-inflammatory diseases.

Published
2016
Full Text
View/download PDF

2. Low DPP4 expression and activity in multiple sclerosis

Author

Rocío Ramos-Medina, Bárbara Alonso, Armanda Casrouge, Janet Vega, Matthew L. Albert, Marta Tejera-Alhambra, Silvia Sánchez-Ramón, Clara de Andrés, Department of Immunology, Hospital General Universitario Gregorio Marañón, Centre d'Immunologie Humaine (CIH), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of neurology, Center Alicia Koplowitz for Multiple Sclerosis of the Community of Madrid, Immunobiologie des Cellules Dendritiques, This work was supported by grants from the Fundación Mapfre, the Fundación Salud 2000 prize for MS research (SSR) and the European Research Council Starting Award (MLA). Marta Tejera-Alhambra received an EFIS grant in 2011 for her stay at Institut Pasteur in Paris., and Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Male, Lymphocyte Activation, 0302 clinical medicine, T-Lymphocyte Subsets, MESH: Receptors, Antigen, T-Cell/metabolism, Immunology and Allergy, Vitamin D, 0303 health sciences, MESH: Middle Aged, Middle Aged, Pathophysiology, MESH: Reproducibility of Results, Phenotype, medicine.anatomical_structure, Biomarker (medicine), MESH: Multiple Sclerosis/genetics, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Adult, MESH: Leukocytes, Mononuclear/metabolism, Immunology, Central nervous system, Receptors, Antigen, T-Cell, Biology, Dipeptidyl peptidase 4, MESH: Phenotype, Multiple sclerosis, 03 medical and health sciences, Sex Factors, MESH: Sex Factors, Clinical activity, MESH: T-Lymphocyte Subsets/immunology, medicine, Humans, MESH: Multiple Sclerosis/metabolism, MESH: Vitamin D/blood, MESH: Dipeptidyl Peptidase 4/genetics, Dipeptidyl peptidase-4, 030304 developmental biology, CD26, MESH: Multiple Sclerosis/diagnosis, Autoimmune disease, MESH: Humans, MESH: Biomarkers/metabolism, Reproducibility of Results, MESH: Adult, Plasma levels, Biomarker, MESH: ROC Curve, medicine.disease, MESH: Male, MESH: Lymphocyte Activation/immunology, MESH: Dipeptidyl Peptidase 4/metabolism, ROC Curve, Leukocytes, Mononuclear, MESH: T-Lymphocyte Subsets/metabolism, MESH: Female, Biomarkers, 030217 neurology & neurosurgery, CD8
Abstract

International audience; Multiple sclerosis (MS) is a prototypic Th1/Th17 chronic autoimmune disease of the central nervous system. Dipeptidyl peptidase 4 (DPP4 or CD26) is a multifunctional molecule involved in autoimmune diseases' pathophysiology. We sought to integrate disparate pieces of data and analyze the plasma levels of sDPP4, DPP activity and DPP4 surface expression on T-cells in 129 MS patients with different clinical forms and 53 healthy controls, across two independent cohorts. Herein, we provide new evidence that sDPP4 concentration and DPP activity are significantly lower in MS patients than controls (p < 0.0001 and p < 0.01, respectively). In contrast, the frequency of circulating CD8(+)DPP4(hi) T-cells (p = 0.02) was increased in MS patients. This is the first study that simultaneously analyzes DPP4 expression and function in a large cohort of MS patients. Our data indicate a putative role for DPP4 in MS pathophysiology and suggest that a deeper understanding of surface versus shed DPP4 biology is warranted.

Published
2013
Full Text
View/download PDF

3. Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry

Author

Matthew L. Albert, Claire De La Calle, Milena Hasan, Pierre-Emmanuel Joubert, Helen K. W. Law, Centre d'Immunologie Humaine (CIH), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Supported by grants from ARC (equipment grant to the Centre d’Immunologie Humaine), the Canceropole, Région Ile de France (C.C.), the EURYI Award from the European Science Foundation and the Young Investigator Award from the European Research Council (M.L.A.)., and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Time Factors, MESH: Fibroblasts/cytology, new methodology, MESH: Fibroblasts/metabolism, Apoptosis, multispectral imaging cytometry, Mutually exclusive events, MESH: Leukocytes, Mononuclear/drug effects, MESH: Apoptosis/radiation effects, Mice, MESH: Image Cytometry/methods, MESH: Animals, Image Cytometry, 0303 health sciences, MESH: Lysosomes/radiation effects, 030302 biochemistry & molecular biology, Chloroquine, Cell biology, Blot, MESH: Autophagy/radiation effects, [SDV.IMM]Life Sciences [q-bio]/Immunology, influenza, MESH: Lysosomes/drug effects, Microtubule-Associated Proteins, Signal Transduction, MESH: Leukocytes, Mononuclear/metabolism, Programmed cell death, MESH: Apoptosis/drug effects, Ultraviolet Rays, MESH: Leukocytes, Mononuclear/cytology, Multiple stress, Biology, Cellular level, 03 medical and health sciences, MESH: Signal Transduction/drug effects, Autophagy, Animals, Humans, MESH: Fibroblasts/radiation effects, MESH: Mice, Molecular Biology, MESH: Chloroquine/pharmacology, 030304 developmental biology, MESH: Humans, MESH: Signal Transduction/radiation effects, MESH: Autophagy/drug effects, MESH: Leukocytes, Mononuclear/radiation effects, MESH: Time Factors, Cell Biology, Fibroblasts, Embryo, Mammalian, MESH: Lysosomes/metabolism, MESH: Fibroblasts/drug effects, MESH: Microtubule-Associated Proteins/metabolism, Leukocytes, Mononuclear, MESH: Ultraviolet Rays, MESH: Embryo, Mammalian/cytology, Lysosomes, Cytometry
Abstract

International audience; Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results