1. The dermis contains langerin+ dendritic cells that develop and function independently of epidermal Langerhans cells
- Author
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Lionel F. Poulin, Adrien Kissenpfennig, Sandrine Henri, Elisabeth Devilard, Bernard Malissen, Béatrice de Bovis, Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Spleen ,MESH: Lymph Nodes ,Kidney ,Mice ,0302 clinical medicine ,MESH: Microscopy, Confocal ,Immunology and Allergy ,MESH: Animals ,MESH: Langerhans Cells ,0303 health sciences ,Microscopy, Confocal ,MESH: Dendritic Cells ,MESH: Kinetics ,integumentary system ,biology ,MESH: Bone Marrow Cells ,hemic and immune systems ,Dermis ,Articles ,Cell biology ,medicine.anatomical_structure ,Antigens, Surface ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Langerhans cell ,Langerin ,MESH: Antigens, Surface ,Immunology ,Bone Marrow Cells ,chemical and pharmacologic phenomena ,Spleen ,MESH: Antigens, CD45 ,Article ,03 medical and health sciences ,MESH: Mice, Inbred C57BL ,MESH: Mannose-Binding Lectins ,medicine ,Animals ,Lectins, C-Type ,MESH: Mice ,030304 developmental biology ,Diphtheria toxin ,Epidermis (botany) ,Dendritic Cells ,MESH: Kidney ,MESH: Dermis ,Mice, Inbred C57BL ,Kinetics ,Mannose-Binding Lectins ,Langerhans Cells ,Humanized mouse ,biology.protein ,Leukocyte Common Antigens ,Lymph Nodes ,Bone marrow ,Epidermis ,MESH: Epidermis ,MESH: Lectins, C-Type ,030215 immunology - Abstract
International audience; Langerhans cells (LCs) constitute a subset of dendritic cells (DCs) that express the lectin langerin and that reside in their immature state in epidermis. Paradoxically, in mice permitting diphtheria toxin (DT)-mediated ablation of LCs, epidermal LCs reappeared with kinetics that lagged behind that of their putative progeny found in lymph nodes (LNs). Using bone marrow (BM) chimeras, we showed that a major fraction of the langerin(+), skin-derived DCs found in LNs originates from a developmental pathway that is independent from that of epidermal LCs. This pathway, the existence of which was unexpected, originates in the dermis and gives rise to langerin(+) dermal DCs (DDCs) that should not be confused with epidermal LCs en route to LNs. It explains that after DT treatment, some langerin(+), skin-derived DCs reappear in LNs long before LC-derived DCs. Using CD45 expression and BrdU-labeling kinetics, both LCs and langerin(+) DDCs were found to coexist in wild-type mice. Moreover, DT-mediated ablation of epidermal LCs opened otherwise filled niches and permitted repopulation of adult noninflammatory epidermis with BM-derived LCs. Our results stress that the langerin(+) DC network is more complex than originally thought and have implications for the development of transcutaneous vaccines and the improvement of humanized mouse models.
- Published
- 2007