1. New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR
- Author
-
Gustavo Luiz Venturelli, Maryella Osório Vargas, Kelly Justin da Silva, Paola Beatriz Navas, Diana Treml, Josias C. Faria, Joana Laura Bischoff, Ana Carolina Maisonnave Arisi, GUSTAVO LUIZ VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC, KELLY JUSTIN DA SILVA, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC, DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC, PAOLA BEATRIZ NAVAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC, MARYELLA OSÓRIO VARGAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC, JOANA LAURA BISCHOFF, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC, JOSIAS CORREA DE FARIA, CNPAF, and ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC.
- Subjects
0301 basic medicine ,Organismo Transgênico ,Beans ,Biology ,Plasmídeo ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,Plasmid ,Quantitative polymerase chain reaction ,Engenharia Genética ,business.industry ,010401 analytical chemistry ,food and beverages ,biology.organism_classification ,DNA extraction ,0104 chemical sciences ,Genetically modified organism ,Biotechnology ,EMB-PV051-1-event ,030104 developmental biology ,Genetically modified organisms ,Duplex (building) ,Phaseolus Vulgaris ,Certified reference material ,Phaseolus ,business ,Feijão ,Food Science ,Plasmids - Abstract
The geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization. Made available in DSpace on 2018-12-03T23:36:23Z (GMT). No. of bitstreams: 1 CNPAF2018fb.pdf: 738975 bytes, checksum: ffa84c1e6d20b31836dba74dff330132 (MD5) Previous issue date: 2018-12-03
- Published
- 2018