1. Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program
- Author
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Kerstin Mätz-Rensing, Charles-Antoine Dutertre, Stefan Cornelius Bohlen, Finnbogi Rutur Thormodsson, Ido Amit, Hadas Keren-Shaul, Noga Zilkha, Stephan Meckel, Christine Pfeifle, Marco Prinz, Antonella Raffo-Romero, Igor Ulitsky, Christine Stadelmann, Eyal David, Christian Scheiwe, Jacopo Vizioli, Franziska van der Meer, Assaf Weiner, Daniel Erny, Adam Balic, Tali Kimchi, Amir Giladi, Florent Ginhoux, Jana Neuber, Francesca Peri, Laufey Geirsdottir, Fadi Sheban, Kaspar Matiasek, SALZET, Michel, Weizmann Institute of Science [Rehovot, Israël], University of Freiburg [Freiburg], University of Edinburgh, Agency for science, technology and research [Singapore] (A*STAR), Max Planck Institute for Evolutionary Biology, Max-Planck-Gesellschaft, Universität Zürich [Zürich] = University of Zurich (UZH), Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Ludwig-Maximilians-Universität München (LMU), Leibniz Institute for Primate Research, German Primate Centre, Innovation Center Iceland (ICELAND), University Medical Center Göttingen (UMG), School of Medicine [Shanghai Jiaotong University], Shanghai Jiaotong University, and Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)
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Primates ,Swine ,Systems biology ,[SDV]Life Sciences [q-bio] ,microglia ,Rodentia ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Evolution, Molecular ,immunology ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Single-cell analysis ,medicine ,Animals ,Humans ,Gene Regulatory Networks ,Genetic Predisposition to Disease ,Receptor ,Gene ,Zebrafish ,030304 developmental biology ,0303 health sciences ,Innate immune system ,Sheep ,single-cell RNA-seq ,Microglia ,Gene Expression Profiling ,Neurodegeneration ,neurodegeneration ,Reptiles ,Neurodegenerative Diseases ,systems biology ,medicine.disease ,[SDV] Life Sciences [q-bio] ,medicine.anatomical_structure ,nervous system ,030220 oncology & carcinogenesis ,Single-Cell Analysis ,Transcriptome ,Neuroscience ,Chickens ,030217 neurology & neurosurgery - Abstract
Erratum inCross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program.Geirsdottir L, David E, Keren-Shaul H, Weiner A, Bohlen SC, Neuber J, Balic A, Giladi A, Sheban F, Dutertre CA, Pfeifle C, Peri F, Raffo-Romero A, Vizioli J, Matiasek K, Scheiwe C, Meckel S, Mätz-Rensing K, van der Meer F, Thormodsson FR, Stadelmann C, Zilkha N, Kimchi T, Ginhoux F, Ulitsky I, Erny D, Amit I, Prinz M.Cell. 2020 Apr 30;181(3):746. doi: 10.1016/j.cell.2020.04.002.PMID: 32359440; International audience; Microglia, the brain-resident immune cells, are critically involved in many physiological and pathological brain processes, including neurodegeneration. Here we characterize microglia morphology and transcriptional programs across ten species spanning more than 450 million years of evolution. We find that microglia express a conserved core gene program of orthologous genes from rodents to humans, including ligands and receptors associated with interactions between glia and neurons. In most species, microglia show a single dominant transcriptional state, whereas human microglia display significant heterogeneity. In addition, we observed notable differences in several gene modules of rodents compared with primate microglia, including complement, phagocytic, and susceptibility genes to neurodegeneration, such as Alzheimer's and Parkinson's disease. Our study provides an essential resource of conserved and divergent microglia pathways across evolution, with important implications for future development of microglia-based therapies in humans.
- Published
- 2019
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