1. Expression and correlation of MALAT1 and SOX9 in non-small cell lung cancer
- Author
-
Liang Yu, Yongqing Hong, Zongren Wan, Wei Zhao, Zhongying Guo, Sheng Chen, Lixin Wang, Ji-Peng Wang, Wei Chen, and Li Zhang
- Subjects
Male ,0301 basic medicine ,Pulmonary and Respiratory Medicine ,Lung Neoplasms ,Kaplan-Meier Estimate ,SOX9 ,03 medical and health sciences ,In vivo ,Carcinoma, Non-Small-Cell Lung ,Biomarkers, Tumor ,Humans ,Immunology and Allergy ,Medicine ,Stage (cooking) ,Lung cancer ,Pathological ,Genetics (clinical) ,Neoplasm Staging ,Messenger RNA ,MALAT1 ,business.industry ,SOX9 Transcription Factor ,medicine.disease ,Tumor Burden ,Up-Regulation ,respiratory tract diseases ,Staining ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Disease Progression ,Cancer research ,Female ,RNA, Long Noncoding ,business - Abstract
Introduction Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related deaths in the world. MALAT1 and SOX9 have important roles in tumour formation and development in several types of cancers. However, little is known about the function and co-relationship of these 2 factors in NSCLC in vivo. Objectives To explore the role of MALAT1 and SOX9 expression relationship, their clinical pathological characteristics and OS on NSCLC patients. Methods Paired of primary lung cancer tissues and the matched tumour adjacent tissues were collected in 121 NSCLC patients. MALAT1 and SOX9 mRNA expression was measured by SYBR green q RT-PCR assay. SOX-9 protein expression was measured by streptavidin-peroxidase (SP) staining method. Results MALAT1and SOX9 expression was higher in NSCLC tissues than the adjacent tissues, and they have positive correlation. Moreover, SOX9 protein expression was higher in NSCLC tissues, especially in MALAT1 mRNA higher expressed NSCLC tissues. MALAT1 and SOX9 mRNA expression were associated with age (x2 =11.474, P = .009), tumour size (x2 =26.839, P = .000), TNM stage (x2 =8.010, P = .046) and LEL. (x2 =53.908, P = .000). NSCLC patients with higher MALAT1 and SOX9 mRNA expression had poorer OS rates. Conclusions MALAT1 and SOX9 could be used as prognostic co-biomarker in NSCLC.
- Published
- 2018