1. Influence of microcurrent on the modulation of remodelling genes in a wound healing assay
- Author
-
Camila Andréa de Oliveira, Andrea Aparecida de Aro, Fernanda Aparecida Sampaio Mendonça, Alexandre Leite Rodrigues de Oliveira, Lucas de Oliveira Fujii, Gláucia Maria Tech dos Santos, Gabriela Bortolança Chiarotto, Thiago Antônio Moretti de Andrade, Daniela Fernanda Dezotti Silva, and Marcelo Augusto Marretto Esquisatto
- Subjects
0301 basic medicine ,medicine.medical_treatment ,Connective tissue ,Electric Stimulation Therapy ,Transforming Growth Factor beta1 ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Genetics ,medicine ,Animals ,Humans ,Viability assay ,Insulin-Like Growth Factor I ,Molecular Biology ,Wound Healing ,biology ,Chemistry ,Growth factor ,Tenascin C ,Connective Tissue Growth Factor ,Tenascin ,Cell migration ,General Medicine ,Molecular biology ,Fibronectins ,Tenomodulin ,Fibronectin ,CTGF ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,NIH 3T3 Cells ,biology.protein ,Fibromodulin - Abstract
The literature has shown the beneficial effects of microcurrent (MC) therapy on tissue repair. We investigated if the application of MC at 10 μA/90 s could modulate the expression of remodeling genes transforming growth factor beta (Tgfb), connective tissue growth factor (Ctgf), insulin-like growth factor 1 (Igf1), tenascin C (Tnc), Fibronectin (Fn1), Scleraxis (Scx), Fibromodulin (Fmod) and tenomodulin in NIH/3T3 fibroblasts in a wound healing assay. The cell migration was analyzed between days 0 and 4 in both fibroblasts (F) and fibroblasts + MC (F+MC) groups. On the 4th day, cell viability and gene expression were also analyzed after daily MC application. Higher expression of Ctgf and lower expression of Tnc and Fmod, respectively, were observed in the F+MC group in relation to F group (p
- Published
- 2021
- Full Text
- View/download PDF