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Aim: To investigate the effect of toothbrushing on different stained Computerd Aided Design/Computer Aided Manufacturing CAD/CAM ceramics. Materials and Methods: Fifty specimens (high translucency zirconia, YZHT, zirconia-reinforced lithium silicate, ZLS, feldspathic, FDL, hybrid ceramic, HC, all from Vita Zahnfabrik) were divided into five groups according to their staining technique. ZLS allowed the composition of two groups: ZLS1 with crystallization and staining together, ZLS2, with the stain firing after the crystallization. YZHT received a stain firing after the sintering process, and FDL received stain firing directly. The HC was stained with acrylic staining, and was light-cured on its surface. The specimens were brushed in total for 150,000 cycles at 2.45 N with 180 strokes/min. Surface measurements to obtain Rz were performed after 50,000, 100,000 and 150,000 cycles, with five evaluation lines (5 mm) per specimen orthogonal to the brushing direction, covering brushed and unbrushed areas. The wear was analyzed using two-way ANOVA and Tukey tests (α = 5%). Scanning electron microscopy (SEM) was performed to access the surface profile. The wear was affected by material (p &lt, 0.001) and time (p = 0.139). Superior wear (µm) was observed for HC (6.6 ± 4.4, 6.8 ± 4.0, 9.2 ± 3.5) compared to ZLS2 (1.0 ± 0.3, 1.2 ± 2.2, 1.3 ± 0.3), YZHT (1.0 ± 0.1, 1.2 ± 0.3, 1.2 ± 0.3), ZLS1 (0.9 ± 0.1, 1.1 ± 0.5, 1.2 ± 0.3) and FDL (0.9 ± 0.1, 0.9 ± 0.1, 1.0 ± 0.2) after 5, 10 and 15 years of simulation, respectively. SEM showed different wear patters for HC with the removal of the glaze layer. HC showed a higher staining wear rate compared to the glass-based and polycrystalline ceramics after 15 years. The extrinsic characterization of feldspathic ceramic showed its superior longevity compared to the evaluated high-translucency zirconia, zirconia-reinforced lithium silicate, and hybrid ceramic.

Little is known about the wear rate of enamel and dentin opposing resin composite following restoration of the occlusal surface in patients with tooth wear. Hence, the aim of this in vitro study was to evaluate the wear of enamel and dentin surfaces when opposing each of three resin composites in a two-body and three-body wear test. The two-body and three-body wear of dentin, enamel, and three resin composites (Clearfil AP-X, Filtek Z250, and Filtek Supreme) opposing four antagonists (stainless steel and three resin composites) were evaluated using the ACTA wear machine. In addition, all the surfaces were evaluated with scanning electron microscopy. The results showed the lowest wear rate in enamel and the highest in dentine, with the composite showing intermediate wear rates. The three-body wear results were not affected by the antagonist and showed pure wear caused by the abrasive food medium. The in vitro enamel/composite wear ratio was 2.5. The results show that tooth wear accelerates as soon as enamel disappears and dentin is exposed to composite resin. Therefore, it is important to protect dentin with a restorative material.

Bone mass increases after error‐loading, even in the absence of osteocytes. Loaded osteoblasts may produce a combination of growth factors affecting adjacent osteoblast differentiation. We hypothesized that osteoblasts respond to a single load in the short‐term (minutes) by changing F‐actin stress fiber distribution, in the intermediate‐term (hours) by signaling molecule production, and in the long‐term (days) by differentiation. Furthermore, growth factors produced during and after mechanical loading by pulsating fluid flow (PFF) will affect osteogenic differentiation. MC3T3‐E1 pre‐osteoblasts were either/not stimulated by 60 min PFF (amplitude, 1.0 Pa; frequency, 1 Hz; peak shear stress rate, 6.5 Pa/s) followed by 0–6 h, or 21/28 days of post‐incubation without PFF. Computational analysis revealed that PFF immediately changed distribution and magnitude of fluid dynamics over an adherent pre‐osteoblast inside a parallel‐plate flow chamber (immediate impact). Within 60 min, PFF increased nitric oxide production (5.3‐fold), altered actin distribution, but did not affect cell pseudopodia length and cell orientation (initial downstream impact). PFF transiently stimulated Fgf2, Runx2, Ocn, Dmp1, and Col1⍺1 gene expression between 0 and 6 h after PFF cessation. PFF did not affect alkaline phosphatase nor collagen production after 21 days, but altered mineralization after 28 days. In conclusion, a single bout of PFF with indirect associated release of biochemical factors, stimulates osteoblast differentiation in the long‐term, which may explain enhanced bone formation resulting from mechanical stimuli., Pulsating fluid flow has distinct temporal impact on pre‐osteoblast behavior and osteogenic differentiation. Initially, PFF increased nitric oxide production, followed in the short‐term by F‐actin stress fiber changes. In the long‐term, PFF did not enhance collagen production, but increased mineralization. This indicates that a single bout of mechanical loading, triggering release of soluble factors, stimulates mineralization in the long‐term.

Made available in DSpace on 2021-06-25T11:14:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Statement of problem: The extrinsic characterization with pigments and glaze application on the surface of ceramic restorations promotes individualization and esthetics. However, whether this characterization is resistant to toothbrushing abrasive wear is unclear. Purpose: The purpose of this in vitro study was to investigate the abrasive wear resistance of characterized and glazed resin-modified ceramic with different surface treatments before characterization. Material and methods: Eighty rectangular specimens (10×8×6 mm) were precision cut from Vita Enamic and randomly divided in 4 groups as per the surface treatment before the characterization technique. These groups included the following: no additional treatment or polishing only, acid etching, airborne-particle abrasion, and self-etching silanization. For each surface treatment, 2 subgroups were created considering the presence or absence of the glaze layer after shade characterization, totaling 8 groups (n=10). The specimens were submitted to toothbrushing simulation (150 000 cycles, 2.45-N, 180 strokes per minute) with a soft and straight toothbrush and a solution of toothpaste and distilled water (250 g/L). After each 50 000 cycles, the wear of the characterized surface was measured by using a contact profilometer in μm which was equivalent to 5, 10, and 15 years of simulated toothbrushing. The rates were analyzed by using 3-way ANOVA and the post hoc Tukey test (α=.05). Scanning electron microscopy analysis was performed to access the surface profile between the evaluated periods. Results: The mean wear rate (Rz) of the characterized surface was affected by the isolated factors, including surface treatment, glaze, and time (P

Made available in DSpace on 2020-12-12T02:17:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study compared the wear resistance of different reinforced glass-ceramics crystalized by conventional or microwave firing. The wear rate of three ceramics [one lithium disilicate ceramic (LD): IPS e.max CAD – Ivoclar Vivadent; and two zirconia reinforced lithium silicates: Suprinity – VITA Zahnfabrik (ZLS1) and Celtra Duo – Dentsply (ZLS2)] crystallized by conventional (c) or microwave (mw) firing protocols were collected according to the contact- [two-body (n = 20/gr)] and contact-free wear tests [three-body (n = 20/gr)]. After wear tests performed on ACTA wear machine, mean surface roughness (Ra) and Scanning Electron Microscopy (SEM) analyzes were performed to evaluate the surface alterations. The wear and roughness data (in μm) were evaluated using two-way ANOVA and Tukey post-hoc test (α = 0.05). Two-body wear test revealed that ZLS1 (1.30 ± 1.79)A showed higher wear rate than LD (0.79 ± 2.15)B and ZLS2 (0.85 ± 0.94)B, regardless the crystallization approach. For three-body test, conventional crystallization (0.62 ± 0.4)A showed higher wear rates than microwave (0.22 ± 0.71)B; while the type of ceramic was not significant. The crystallization protocol (p < 0.001) and ceramic material (p = 0.001) affected the surface roughness in the three-body; whereas the two-body test, only the crystallization protocol (p = 0.046). SEM analysis showed a similar and smother surface morphology for LD and ZLS2 compared to ZLS1. Conventional crystallization showed materials more prone to wear than the microwave, in the presence of food bolus. Therefore, the microwave crystallization can be suggested as an alternative to improve the evaluated glass-ceramics wear resistance. Post-Graduate Program in Restorative Dentistry (Prosthodontic) Department of Dental Materials and Prosthodontics São Paulo State University (Unesp/SJC) Institute of Science and Technology, Engenheiro Francisco José Longo Avenue, 777 Department of Dental Materials Science Academic Centre for Dentistry Amsterdam (ACTA) Universiteit van Amsterdam and Vrije Universiteit., Gustav Mahlerlaan #3004 Post-Graduate Program in Restorative Dentistry (Prosthodontic) Department of Dental Materials and Prosthodontics São Paulo State University (Unesp/SJC) Institute of Science and Technology, Engenheiro Francisco José Longo Avenue, 777 FAPESP: 2019/06458-5

This study investigated the effect of surface roughness (polished vs. CAD/CAM milling roughness simulation) on the fatigue behavior of five dental ceramics for manufacturing CAD/CAM monolithic restorations. Specimens of five dental ceramics (FC- feldspathic; PICN- polymer-infiltrated ceramic-network; ZLS- zirconia-reinforced lithium silicate glass-ceramic; LD-lithium disilicate glass-ceramic; YZ-yttria-stabilized tetragonal zirconia polycrystal), to be tested under fatigue (12 × 12 × 1.2 mm3), were assigned into two groups according to surface treatment: polished ‘p’ (#2500-grit SiC papers) and CAD/CAM milling roughness simulation ‘gr’ (grinding with #60-grit SiC paper). The fatigue test was performed through the stepwise method (40N–660N; step of 20N; 10,000 cycles/step; 20 Hz frequency). Roughness, topographic and fractographic analyses were performed. The fatigue data were analyzed by Kaplan-Meier and Mantel-Cox (Log rank), and Pearson correlation was used to correlate roughness vs. fatigue data. CAD/CAM milling roughness simulation led to significantly (p < 0.05) greater roughness (Ra and Rz), promoting a more irregular topography with scratches and grooves, and led to a lower fatigue performance for all the tested ceramics. Fractographic analysis depicted the origin of failure at the higher stress concentration side, the side subjected to tensile tension during the fatigue test. The CAD/CAM milling roughness simulation significantly decreased the fatigue performance of the evaluated ceramic materials.

Made available in DSpace on 2018-12-11T17:38:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01 Statement of problem: Computer-aided design and computer-aided manufacturing (CAD-CAM) restorations are in contact with the antagonist tooth, either a natural tooth or a restoration. Therefore, clinicians should be aware of the wear resistance of CAD-CAM materials and the wear behavior of the antagonist. Purpose: The purpose of this in vitro study was to evaluate the wear resistance and abrasiveness of CAD-CAM materials. Material and methods: In a 2-body wear test, the materials IPS e.max CAD (Ivoclar Vivadent AG), Vita Suprinity (Vita Zahnfabrik), Lava Ultimate (3M ESPE), Vita Enamic (Vita Zahnfabrik), and Lava Plus (3M ESPE) acted as abraders and, together with bovine enamel, also as antagonists. Each antagonist wheel ran against each abrader wheel for 200 000 cycles, with a spring force of 15 N, and at a rotational speed of 1 Hz in distilled water. The wear rate was determined with a surface profilometer. The surfaces were observed with scanning electron microscopy, and their hardness, coefficient of friction, and roughness were evaluated. Results: Lava Plus and IPS e.max CAD exhibited the highest potential for wear of Lava Ultimate. These 2 materials, together with Vita Suprinity, provided the highest wear of enamel and Vita Enamic. Vita Suprinity and IPS e.max CAD had higher wear than Lava Plus, and the inverse was also true. Vita Enamic and Lava Ultimate were among the materials that caused the lowest wear of enamel and all other evaluated materials. Scanning electron microscopy images revealed that except for Lava Ultimate, all other materials damaged enamel, in which Vita Suprinity and IPS e.max CAD were more aggressive when sliding against the materials. Lava Plus had the greatest hardness, followed by Vita Suprinity and IPS e.max CAD, Vita Enamic, and then Lava Ultimate. The coefficient of friction varied from 0.42 to 0.53. The Vita Enamic and Lava Ultimate showed the highest surface roughness. Conclusions: The nanofilled composite resin and polymer-infiltrated ceramic were more antagonist-friendly (whether enamel or CAD-CAM material) than glass-ceramics and zirconia. Care should be taken when selecting the material that will contact mainly with glass-ceramics. Hardness should also be considered when selecting a material. Postgraduate student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP) Postdoctoral student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP) Engineer Department of Dental Materials Science Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam; and Vrije University Amsterdam Professor Department of Dental Materials Science Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam; and Vrije University Amsterdam Associate Professor Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP) Postgraduate student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP) Postdoctoral student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP) Associate Professor Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)

The aim of this study is to test the cleaning effect and surface modification of a new implant surface treatment on explanted dental implants and titanium discs. It is a modified air powder abrasive (APA) treatment applied using osteoconductive powders. Twenty‐eight in vitro Ca‐precipitated organic film‐coated titanium discs and 13 explanted dental implants were treated. In a 2‐step approach, 3 powders were used: hydroxylapatite (HA) and biomimetic calcium phosphate (BioCaP), which are osteoconductive, and erythritol, which is not. APA treatment was applied. (Air pressure: 2.4 bar; water flow for cleaning: 41.5 ml/min, for Coating 1: 2.1 ml/min, and for Coating 2: 15.2 ml/min.) The test groups were as follows: Group 1: HA cleaning + BioCaP Coating 1; Group 2: HA cleaning + BioCaP Coating 2; Group 3: erythritol cleaning + BioCaP Coating 1; Group 4: erythritol cleaning + BioCaP Coating 2; Group 5: HA cleaning; Group 6: erythritol cleaning; and control: no powder. Cleaned areas were calculated by point counting method. Surface changes and chemical content were evaluated using light microscopy, scanning electron microscopy, and energy‐dispersive X‐ray spectroscopy. Cleaning effect between groups was compared by a pairwise Student's t test. The significance level was fixed at p

Regardless the materials properties, the vast majority of ceramic restorations could require an individualization through the extrinsic staining to improve aesthetics. This study aimed to compare the staining wear durability of different monolithic ceramics. Specimens of high translucent zirconia (YZHT), zirconia reinforced lithium silicate (ZLS), hybrid ceramic (HC) and feldspathic ceramic (FLD) were divided in five groups according to each material staining technique. The ZLS ceramic was tested with stained prior (ZLS1) and after crystallization (ZLS2). All specimens were extrinsically characterized, i.e. stained, and crystallized or sintered in specific ovens, according to the manufacturer's recommendation. The specimens were submitted to three-body wear tests in ACTA wear machine, simulating the presence of food bolus and antagonist (pH 7, 15 N, 1 Hz). The wear rate of the stain surface was determined after 5 intervals of 200,000 cycles, using a profilometer. The ceramic surface before and after staining, and after wear were inspected by Scanning Electron Microscopy (SEM). The wear rates were analyzed using two-way ANOVA and post-hoc Tukey test. The wear rates of the staining were affected by ceramic and the number of cycles (P 0.001). 100% of staining was removed after 200,000 cycles for HC, and after 600,000 cycles for YZHT and ZLS1. Staining of ZLS2 and FLD remained on ceramic surface even after 1,000,000 cycles. Furthermore, FLD showed a significant higher staining durability than ZLS2. SEM revealed different surface morphologies for each group with and without staining and after the wear test. Ceramics with fired staining showed higher durability compared to the polymerized one. The feldspar ceramic presented superior staining durability, followed by zirconia reinforced lithium silicate and high translucent zirconia. The conventional two steps staining technique showed improved durability for zirconia reinforced lithium silicate.

Hybrid ceramic is a promising material for monolithic restorations that could require an individualization through the extrinsic staining to improve aesthetics. Due to the possibilities to treat this ceramic prior to staining, this study evaluated the wear resistance of surface treatments prior to staining and glazing a hybrid ceramic. Thirty-two specimens (Vita Enamic) were divided into 8 groups according to the surface treatment prior to the staining (Polishing: Pol, Acid etching: Ac, Sandblasting with Al2O3: Sd or Self-etching silane: Ses) and glaze application (with: gl or without: gl). The specimens were submitted to the ACTA wear machine simulating the presence of food bolus and antagonist. The wear rate of the stain was determined after 7 intervals of 20,000 cycles, using a profilometer. The surface before and after staining, and after wear were inspected using Scanning Electron Microscopy (SEM). The rates were analyzed using three-way ANOVA and Tukey test. The wear was affected by surface treatment, glaze application and number of cycles (p

Objective: The use of knitted, polypropylene meshes for the surgical treatment of pelvic organ prolapse (POP) is frequently accompanied by severe complications. Looking for alternatives, we studied the potential of three different electrospun matrices in supporting the adhesion, proliferation, and matrix deposition of POP and non-POP fibroblasts, the most important cells to produce extracellular matrix (ECM), in vitro. Study design: We electrospun three commonly used medical materials: nylon; poly (lactide-co-glycolide) blended with poly-caprolactone (PLGA/PCL); and poly-caprolactone blended with gelatin (PCL/Gelatin). The matrices were characterized for their microstructure, hydrophilicity, and mechanical properties. We seeded POP and non-POP fibroblasts from patients with POP and we determined cellular responses and ECM deposition. Results: All matrices had >65% porosity, homogenous microstructures, and close to sufficient tensile strength for pelvic floor repair: 15.4 ± 3.3 MPa for Nylon; 12.4 ± 1.6 MPa for PLGA/PCL; and 3.5 ± 0.9 MPa for PCL/Gelatin. Both the POP and non-POP cells adhered to the electrospun matrices; they proliferated well and produced ample ECM. Overall, the best in vitro performance appeared to be on nylon, presumably because this was the most hydrophilic material with the thinnest fibers. Conclusion: Electrospun nanofibrous matrices show feasible mechanical strength and great biocompatibility for POP and non-POP fibroblasts to produce their ECM in vitro and, thus, may be candidates for a new generation of implants for pelvic floor repair. Further studies on electrospun nanofibrous matrices should focus on mechanical and immunological conditions that would be presented in vivo.

Objective This study investigated the influence of hydrofluoric acid (HF) etching at different concentrations on the fatigue failure load of adhesively cemented feldspathic ceramic discs (Vita Mark II). Besides, their effect on the micromorphology of ceramic surface was investigated. Methods Eighty ceramic discs (ϕ = 10 mm; thickness = 1.5 mm) were cemented to epoxy supporting discs (ϕ = 10 mm; thickness = 2.0 mm) using different surface conditioning methods (n = 20): nonetched control (CTRL), or etched for 60 s with different HF concentrations: 1% (HF1), 5% (HF5), or 10% (HF10). All the ceramic discs received a silane application (Monobond Plus). The epoxy discs were etched with 10% HF for 60 s and received a primer coating (Multilink Primer A + B). Adhesively cementation was performed (Multilink Automix), and the assemblies (ceramic discs/epoxy discs) were subjected to cyclic loads in water by a staircase approach (500,000 cycles; 20 Hz; initial load = 290 N; step size = 30 N). Fatigue failure load data were analyzed using 1-way ANOVA and post-hoc Tukey’s tests (α = .05). Results Mean failure load of the HF5 group (255.0 ± 23.0 N) was significantly lower; HF1 group (301.7 ± 71.0 N) presented intermediate values, and the highest values were achieved in CTRL (351.7 ± 13.4 N) and HF10 (341.7 ± 20.6 N) groups. All the failures were radial cracks starting from the bonding surface. Significance In terms of fatigue failure load, etching with 1% and 5% HF had a deleterious effect on the fatigue behavior of an adhesively cemented feldspathic ceramic, while 10% HF had no negative influence.

Various studies have shown that glass ionomers are susceptible to brittle fracture and acid conditions and that they undergo long-term changes in their mechanical properties. Little information is available on how brittleness, acid susceptibility, and long-term changes are reflected in the wear characteristics of glass ionomers. The purpose of this study was to evaluate long-term changes in conventional glass ionomers, metal-reinforced glass ionomers (including a cermet), and (light-curing) resin-modified glass ionomers by wear experiments simulating the wear process in occlusal contact-free areas. The wear tests were conducted periodically over a period of one year. In addition, wear was determined after one year at a pH of 5 or 6, for assessment of acid susceptibility, and at a condition as found in the occlusal contact areas. All materials showed high early-wear rates which decreased significantly during the one-year test period. This long-term process may be related to a slow progression of the acid-base reaction extending over several months. At each stage, the resin-modified glass ionomers wore significantly faster than the acid-base setting glass ionomers. Most of these materials were not affected at a pH of 6.0, while at a pH of 5.0 only the conventional and the metal-reinforced glass ionomers showed increased wear. Direct contacts with the antagonist led to a significant increase in wear in comparison with contact-free wear, probably as a result of sub-surface fatigue phenomena. In view of the unfavorable wear characteristics of the resin-modified glass ionomers and the high early wear of the conventional glass ionomers, including the metal-reinforced glass ionomers, it was concluded that none of these materials can yet be recommended for use in high-stress-bearing situations.

The influence of sliding action of the antagonist on occlusal three-body wear of composites and an amalgam was investigated in vitro by gradual change in the distance between the opposing substrates. When the distance was decreased from 10 μm to approximately 3 μm, wear increased significantly by a factor of two to three and was exclusively of erosive nature. At a slurry-film thickness of approximately 1 μm, direct contacts between the antagonist and protruding composite filler particles started to occur. This consequently slowed the erosive wear. Ultimately, direct contact phenomena predominated, decreasing the wear rate of the various materials to different degrees. Loss of material due to subsurface fatigue could not be demonstrated with a contact pressure of 45 MPa at which the experiments were performed. From this study, it can be concluded that minor alterations of the food-film thickness at the contact areas result in considerable changes in wear rates and wear-rate ranking of composite materials, which may partly explain inconsistencies among clinical trials.