1. Evaluation of the cytotoxic effects of a new Harvard MTA compared to MTA Flow and ProRoot MTA on human gingival fibroblasts
- Author
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Abdel-Rahman Youssef and Samia Elsherief
- Subjects
Mineral trioxide aggregate ,MTT assay ,Biocompatibility ,Chemistry ,Cytotoxicity ,RK1-715 ,030206 dentistry ,Andrology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Dentistry ,MTA ,Medicine ,Cytotoxic T cell ,Original Article ,Gingival fibroblasts ,Viability assay ,Proroot mta ,General Dentistry ,Fetal bovine serum - Abstract
Background: Biocompatibility is an essential property for any dental root repair material that may interact with the surrounding periodontal tissues. We hypothesise that the three mineral trioxide aggregate (MTA) restorative brands ProRoot MTA, MTA Flow and Harvard MTA have similar biocompatibility. To test this hypothesis, we compared the cytotoxic effects of these materials on human gingival fibroblast (GF). Methods: MTA cements were prepared, and after completion of setting, they were incubated in Dulbecco's Modified Eagle Medium for 1 day or 4 days to obtain low and high concentrations of MTA elutes respectively. The elutes of MTA supplemented with fetal bovine serum were added to GF and incubated for 3 days at 37 °C and 5% CO2. Untreated cells were used as control. The cell viability was assessed using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay at 24, 48 and 72 h. Results: After 24 h, the MTT assay showed that both 1- and 4-day elutes of MTA flow and Harvard MTA reduced cell viability significantly compared to control (P
- Published
- 2021