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Recently, the siderophores have opened new horizons in nanomedicine. The current study aimed to design a theranostic platform based on superparamagnetic iron oxide nanoparticles-pyoverdine (SPION/PVD) conjugates bound to MUC1 aptamer (MUC1Apt) and loaded with doxorubicin (DOX) as an anti-cancer agent. The SPION/PVD complex was covalently conjugated to MUC1Apt and loaded with DOX to prepare a targeted drug delivery system (SPION/PVD/MUC1Apt/DOX). The investigation of cellular cytotoxicity and uptake of formulations by MTT and flow cytometry in both MUC1 positive (C26) and MUC1 negative (CHO) cell lines revealed that MUC1Apt could improve both cellular uptake and toxicity in the C26 cell line. The evaluation of tumor-targeting activity by in vivo bio-distribution showed that the targeted formulation could enhance tumor inhibitory growth effect and survival rate in C26 tumor-bearing mice. Furthermore, the potential of synthesized SPION/PVD/MUC1Apt/DOX complex as diagnostic agents was investigated by magnetic resonance imaging (MRI) which improved the contrast of tumor site in MRI. Our findings confirm that aptamer-targeted PVD chelated the SPION as a diagnostic agent and loaded with DOX as a chemotherapeutic drug, would be beneficial as a novel theranostic platform.

Malignant glioma cells are generally insensitive to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-induced apoptosis. In this research, we designed a system containing mitoxantrone (MTX) which is loaded into poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) in order to increase sensitivity of glioblastoma cancer cell to TRAIL plasmid. PLGA NPs were prepared using a water-in-oil-in-water (W/O/W) double emulsification and solvent evaporation technique and characterized. Synergistic cytotoxicity effect was assessed by both MTT and annexin V-FITC and PI analysis against GL-261 cancer cell line. The combination treatment with PLGA-MTX and TRAIL plasmid showed more cytotoxic effect on GL-261 cancer cell line (cell viability = 16%) compared to MTX-PLGA alone (cell viability 38%) and TRAIL alone (cell viability = 87%). It concluded that PLGA-MTX can be considered a potential agent to sensitize glioblastoma cancer cell to TRAIL.

In recent years, many stimuli-triggered drug delivery platforms have been designed to deliver drugs accurately to specific sites and reduce their side effects, improving "on-demand" therapeutic efficacy. Adenosine-5'-triphosphate (ATP)-responsive drug delivery methods are examples of these systems that use ATP molecules as a trigger for delivery of therapeutic agents. Since intra- and extra-cellular ATP concentrations are significantly different from each other (1-10 mM and

Nucleolin or C23, is one of the most abundant non-ribosomal phosphoproteins of nucleolus. However, in several cancers, nucleolin is highly expressed both intracellularly and on the cell surface. So, it is considered as a potential target for the diagnosis and cancer therapy. Targeting nucleolin by compounds such as AS1411 aptamer can reduce tumor cell growth. In this regard, interest has increased in nucleolin as a molecular target for overcoming cancer therapy challenges. This review paper addressed recent progresses in nucleolin targeting by the G-rich AS1411 aptamer in the field of cancer therapy mainly over the past three years.

This study demonstrated a chemotherapy drug-free delivery system for breast cancer treatment based on a simple DNA nanostructure composed of sequence 1 containing ATP and AS1411 aptamers and sequen...

Clinical trials of cholesterol ester transfer protein (CETP) peptide vaccine were stopped after disappointing results in humans due to the inadequacy of adjuvant aluminum hydroxide in stimulating the immune response against the self-antigen of CETP. To increase the efficacy of the CETP vaccine, we developed a novel liposomal form of tetanus toxoid-CETP (TT-CETP) peptide (Lip CETP) with well-characterized properties and high encapsulation efficiency. The vaccine efficacy against atherosclerosis was evaluated in rabbits challenged with a high cholesterol diet. Rabbits were immunized with Lip-CETP or liposome containing CETP with CpG ODN (Lip CETP/CpG). Control groups received empty liposomes or buffer. Anti-TT-CETP specific antibodies in serum were determined and gene expression of cytokine IFN-γ and IL-4 were measured in blood peripheral mononuclear cells. Therapeutic response was evaluated by titration of plasma lipoproteins during the study and pathologic analysis of aorta atherosclerotic lesions at the end. Lip-CETP/CpG elicited strong anti-TT-CETP antibodies and a higher IFN-γ level than the buffer. IL-4 was lower than the buffer in all vaccinated groups. Plasma lipoproteins showed no significant difference in the studied groups. Atherosclerosis thickness grade of the aorta was lower than the buffer group (

Studies have shown that microRNAs affect the development of tumors. In many cases, they can be applied as biomarkers for the diagnosis of cancer; therefore, simple and sensitive analytical methods for detection of miRNAs are necessary. In this study, miR-141, which is used to diagnose several types of cancer, was detected in water and serum samples using a biosensor designed based on streptavidin-coated magnetic beads (SMBs), complementary sequences of miR-141 and PicoGreen as the fluorescent dye. The method is relatively fast and simple. Briefly, in the presence of miR-141, the complementary sequence forms a DNA:RNA double–strand on the surface of SMBs with intercalated PicoGreen. Upon attachment of the PicoGreen, the fluorescence intensity increased significantly (1000-fold). In the absence of a target, only single-stranded DNA (complementary strand of miR-141) existed on the surface of the SMBs. The fluorescence of the PicoGreen was low. The results revealed that the detection limits of the biosensor for miR-141 were 70 and 113.8 nmol L−1 in deionized water and serum samples, respectively.

Detection and quantitation of bisphenol A (BPA) level in food samples and environment are of great significance. In this research work, a new electrochemical aptasensor was developed for ultrasensitive detection of BPA, based on MWCNT/SiO2@Au nanocomposite. Molecular dynamic (MD) simulation was carried out in order to better understand the interaction between the target molecules and the aptamer from molecular point of view. The detection strategy of the proposed electrochemical aptasensor, is based on [Fe (CN)6]3-/4- as a label free redox probe. In the absence of BPA molecules, the aptamers remain unfold so that the long tunnels for receiving the [Fe (CN)6]3-/4- redox probe are formed at the surface of the electrode. But, upon addition of BPA molecules, strong interactions between the BPA molecules and the aptamer are occurred. Therefore, the entrance of the tunnels is closed which results in a hard electron exchange between the redox probe and the electrode surface. Therefore, the electrochemical signal is decreased in the presence of BPA molecules. The proposed electrochemical aptasensor shows a high selectivity toward BPA molecules with a limit of detection (LOD) as low as 10 pM. The fabricated electrochemical aptasensor was successfully applied for detection of BPA in food samples.

Herein, an electrochemical aptasensor is described for detection of ampicillin (Ampi). The sensing strategy is based on the application of a ladder-shaped DNA structure as a multi-layer physical block on the surface of gold electrode. Attributing to the electrostatic repulsion and physical prevention of the ladder-shaped DNA structure, ultrasensitive detection of Ampi was achieved with a detection limit as low as 1 pM. In the presence of Ampi, the ladder-shaped DNA structure is disassembled and detached from the electrode surface. This leads to the high access of [Fe(CN)6]3-/4- as a redox indicator to the electrode surface and a strong redox peak. The aptasensor response for Ampi detection was in the linear range from 7 pM to 100 nM with the detection limit of 1 pM. The presented analytical strategy showed its application in detecting Ampi in the spiked milk samples with satisfactory performance. This work can be easily expanded for different targets by alternating the corresponding aptamers.

Single-stranded DNA aptamers recognizing CD70 molecule overexpressed in some tumor cell lines was isolated by means of systematic evolution of ligands by exponential enrichment (SELEX). Both purified CD70 protein and CD70-expressing cells were used to isolate target-specific aptamer. After certain rounds of protein-based SELEX and cell-SELEX (ten and seven rounds, respectively), Aptamer 928 (hereafter Apt928) with high affinity and specificity for CD70 was obtained. The specific binding of the aptamer to its target could block interaction of CD70 with CD27 (known as CD70 receptor). Dissociation constant of Apt928 was calculated to be 66 nmol L−1. Moreover, ATTO 674N-labeled Apt928 was applied as a fluorescent aptasensor for rapid and sensitive detection of SKOV-3 cells as CD70-positive cells. The detection limit of this aptasensor was 14 cells mL−1.

To explore the effect of combination therapy of epirubicin (Epi) and melittin (Mel) to cancer cells, calcium carbonate nanoparticles (CCN), as carriers, were developed which were modified with MUC1-Dimer aptamers as targeting agents. Both Epi and Mel were delivered at the same time to cancer cells overexpressing the target of MUC1 aptamer, mucin 1 glycoproteins (MCF7 and C26 cells). CCN were prepared with a water-in-oil emulsion method. Epi and Mel were separately encapsulated in CCN and the nanoparticles were modified with MUC1-Dimer aptamers. In vitro studies, including MTT assay, flow cytometry analysis and fluorescence imaging were applied to investigate the targeting and cell proliferation inhibition capabilities of MUC1-Dimer aptamer-CCN-Mel complex and MUC1-Dimer aptamer-CCN-Epi complex in the target (MCF-7 and C26 cells) and nontarget (HepG2) cells. Also, the function of the developed complexes was analyzed using in vivo tumor growth inhibition. The release of Epi from MUC1-Dimer aptamer-CCN-Epi complex was pH-sensitive. Cellular uptake studies showed more internalization of the MUC1-Dimer aptamer-CCN-Epi complex into MCF-7 and C26 cells (target) compared to HepG2 cells (nontarget). Interestingly, the MUC1-Dimer aptamer-CCN-Mel complex and MUC1-Dimer aptamer-CCN-Epi complex indicated very low toxicity as compared to target cells. Moreover, co-delivery of Epi and Mel using the mixture of MUC1-Dimer aptamer-CCN-Mel complex and MUC1-Dimer aptamer-CCN-Epi complex exhibited strong synergistic cytotoxicity in MCF-7 and C26 cells. Furthermore, the presented complexes had a better function to control tumor growth in vivo compared to free Epi.

Ochratoxin A (OTA) as the most dangerous mycotoxin is produced by Aspergillus Ochraceus and Penicillium verrucosum. OTA can be found in beverages and foodstuffs that induces the teratogenic, nephrotoxic, carcinogenic, and immunosuppressive effects on humans. Hence, developing highly sensitive methods for its detection is of great importance. Herein, a novel aptasensor was designed for the label-free monitoring of the ultra-low OTA levels by a combination of the superiority of aptamers and long-range orientational order of liquid crystals (LCs). The aptasensing strategy was based on the conformational switch of the immobilized π-shaped DNA structure on the glass substrate in presence of the target. A shift in the orientation of LCs from random to homeotropic state led to the apparent alteration of the optical appearance of the aptasensor platform from bright to dark. The LC-based aptasensor especially detects OTA at the ultra-trace level as low as 0.63 aM with comparable selectivity. The aptasensor could detect OTA successfully in the grape juice, coffee, and human serum samples. The LC-based aptasensor paves a way for developing portable and real-time sensing probes with high performance for food safety control and clinical application.

In spite of huge developments in cancer treatment, versatile combinational formulations of different chemotherapeutic agents to enhance anticancer activity while reducing systemic toxicity still remains a challenge. In this regard, in the current study, an amphiphilic hyaluronic acid-b-polycaprolactone diblock copolymer was synthesized using “click chemistry”. The synthesized copolymer was self-assembled to form polymersomal structures for co-encapsulation of hydrophilic doxorubicin (DOX) and hydrophobic camptothecin (CPT) in their interior aqueous compartment and their bilayer, respectively with 1:10 and 1:1 ratios. The prepared polymersomal combinational formulation surrounded by hyaluronic acid brush as hydrophilic segment, could provide active targeting of the system against CD44 marker expressed on the surface of cancerous cells. The hyaluronic acid shell could also provide flexible chemistry for the conjugation of therapeutic FOXM1-specific DNA aptamer (Forkhead Box M1; against transcription factor FOXM1) on the surface of polymersomes in order to further suppress cancerous cell proliferation. The obtained results demonstrated that the prepared co-formulation provided sustained, controlled release of the entrapped drugs during 200 h. In vitro cytotoxicity experiments on non-small cell lung cancer, A549 and SK-MES-1 cell lines, demonstrated that the co-formulation of DOX and CPT provided synergistic effect and significantly higher cytotoxicity in comparison with free drugs. The cytotoxicity experiment also indicated that the aptamer conjugation on the co-formulations surface could significantly increase the cytotoxicity and induce apoptosis in combination therapy on both A549 and SK-MES-1 cell lines while aptamer-conjugated blank NPs did not show any cytotoxicity which emphasizes on the sensitization capability of the FOXM1 DNA aptamer against non-small cell lung cancer. Furthermore, it was shown that the co-formulation with or without aptamer renders the formulation specific tumor accumulation in vivo 24 h post-administration, assisting the combination synergy observed in vitro to be translated to in vivo antitumor efficacy. This combinatorial delivery platform strongly offers a novel approach for the synergistic controlled transportation of several chemotherapeutics for the treatment of non-small cell lung cancer.

Hybrid vesicular systems (lipopolymersomes) are promising platforms for minimizing the liposomes and polymersomes disadvantages in terms of chemotherapeutic transportation. In this regard, lipopolymersome has been designed to integrate the advantage of both polymersomes and liposomes to enable better structural integrity of the bilayer after encapsulation of hydrophobic drugs while maintaining the soft nature of liposomes, superior serum stability, and high encapsulation efficiency of cargos in the bilayer segment. In the present study, we reported preparation and characterization of five camptothecin (CPT)-loaded lipopolymersomal formulations composed of poly (ethylene glycol)–poly (lactic acid) (PEG-PLA) and dipalmitoylphosphatidylcholine (DPPC) at different molar ratios using film rehydration method. Afterward, the preferred formulation was tagged with AS1411 DNA aptamer in order to evaluate the therapeutic index using nucleolin-positive colon cancer cell lines (HT29 and C26). The obtained data indicated that the prepared CPT-loaded lipopolymersome at a PEG-PLA: DPPC ratio of 75:25 exhibited superior stability and high loading capacity compared to other systems. Moreover, high cytotoxicity of the aptamer-targeted lipopolymersome and increased tumor accumulation were observed in comparison with non-targeted one. The designed polymer-rich lipopolymersomal platform offers bright future for the development of potent nanomedicine against cancer.

One of the most common and important pathogenic bacteria is Staphylococcus aureus (S. aureus) which is known as a foodborne illness all over the world. The detection of micrococcal nuclease (MNase) can act as a unique diagnostic biomarker for the identification of S. aureus. So far, various complex methods have been introduced for the evaluation of S. aureus bacterium. However, they have different limitations such as labor-intensive, inaccurate results and time-consuming procedures. Thus, it is of particular attention to develop fast, easy, simple and more approachable detection methods based on nanotechnology and MNase detection. In this review, recent advances and modern techniques of ultrasensitive biosensors based on quantum dots (QDs), noble metal and magnetic nanoparticles (NPs), graphene oxide (GO) nanosheets, and also transfer energy strategy have been discussed for the identification of MNase activity and S. aureus contamination. Besides, advantages and disadvantages of different types of fluorescent, phosphorescent and colorimetric biosensors have been discussed.

The progress in advanced materials using nanoscale components can be conducted by an innovative idea involving combining nanotechnology approaches with supramolecular chemistry. This concept which is called self-assembly, is employed for formation of nanoscale self-assembled architectures with desirable properties. Self-assembly has emerged as a potent procedure for controlling the structure and characteristics of ensembles. Among self-assembled structures, polymersomes have revealed prodigious potential in drug delivery and development of smart nanomedicine due to their morphological similarities to cellular membranes and viral capsids. Moreover, their functional, tunable and stable membrane render them more advantageous to their lipid counter parts. Polymersomes have been used as different therapeutic carriers as drugs and imaging agents, as well as nanoreactors and artificial organelles. Here we review different basis of self-assembling polymersomes and their properties, the synthesis of various amphiphilic copolymers for vesicles preparation and potential applications of smart controlled release vesicles.

Herein, a novel turn-off fluorescent aptasensor was developed for selective detection of ampicillin (AMP) at picomolar level based on 3,4,9,10-perylenetetracarboxylic acid diimide (PTCDI) as an affordable and low-cost fluorophore. This aptasensor was designed using aptamer, its complementary strand (CS) and gold nanoparticles (AuNPs). The principle of the sensing method is a decrease in the fluorescence intensity of PTCDI in the presence of free CS. Following the addition of AMP, Aptamer/CS-modified AuNPs releases CS and so, the fluorescence intensity of PTCDI is reduced. The designed analytical method indicated a good linear range from 100 pM to 1000 pM and a limit of detection (LOD) of 29.2 pM was obtained. Furthermore, the sensing strategy indicated satisfactory results for the detection of AMP in the spiked human serum samples. By changing the sequences of aptamer and its CS, the presented analytical approach can be easily applied for detection of other antibiotics.

Breast cancer is the second leading cause of cancer deaths in women and, due to its diverse morphological features, variable clinical outcome and response to different therapeutic options, is known as a highly heterogeneous disease. Aptamers are a class of therapeutic oligonucleotides that are rapidly generated through the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process. The unique properties of aptamers such as high binding affinity and selectivity, low or no immunogenicity and toxicity, low cost and thermal stability make them good potential pharmaceutical agents. In this review, we present the recent progress of aptamer application in targeted delivery systems for imaging and treatment of breast cancer. Furthermore, some analytical approaches such as electrochemical and optical aptasensors are introduced for the detection and diagnosis of breast cancer. Finally, we discuss the problems and challenges of aptamer application in this field.

Simultaneous drug and gene delivery to cancer cells has been introduced to provide advantages of the synergistic effects of gene to sensitize the cancer cells to chemotherapeutic agent. In the current study, nucleolin-targeted co-delivery system, based on PEGylated rod-shaped mesoporous silica NPs was developed as a biocompatible nanocarrier for simultaneous delivery of camptothecin and survivin shRNA-expressing plasmid (iSur-DNA) to colon adenocarcinoma. The structural characterization including hydrodynamic radius and morphological characteristics of the prepared system demonstrated the mesoporous rod-shaped structure of the prepared system with 100–150 nm diameter. Camptothecin was loaded into the rod-shaped MSN NPs with encapsulation efficiency of 32%. At the next stage, the prepared camptothecin-loaded system was PEGylated and then iSur-DNA was condensed with C/P ratio of 6 to form PEG@MSNR-CPT/Sur. Then, the prepared camptothecin-iSur-DNA loaded PEGylated rod-shaped mesoporous silica NPs were tagged with AS1411 DNA aptamer (Apt-PEG@MSNR-CPT/Sur) in order to provide selective therapy against colorectal adenocarcinoma. The obtained results showed that the prepared platform controlled the release of anticancer drug, camptothecin. The experimental results indicated potent synergistic effect of iSur-pDNA and CPT in in vitro cytotoxicity, apoptosis induction and in vivo antitumor effect. In addition, tagging the system with AS1411 DNA aptamer facilitated drug uptake into nucleolin positive colorectal cancer cells leading to higher cellular toxicity and apoptosis induction in C26 cells compared to nucleolin-negative CHO cell line. Apt-PEG@MSNR-CPT/Sur system significantly supressed tumor growth rate in C26 tumor bearing mice while improving survival rate and pharmacokinetics of the platform in comparison with PEG@MSNR-CPT and PEG@MSNR-CPT/Sur. It could be concluded that the developed nucelolin targeted nanomedicine for co-delivery of camptothecin and iSur-DNA could serve as a versatile nanotherapeutic system against colorectal cancer.

In this study, a fluorescent aptasensor is described for ultrasensitive detection of prostate-specific antigen (PSA) using DNA triangular prism as a platform for attachment of fluorophore (PicoGreen, PG), streptavidin magnetic beads (SMBs) and RecJf exonuclease as enhancers of fluorescence difference between presence and absence of target. Presence of PSA leads to the formation of the DNA origami. So, a strong fluorescence is observed following the addition of PG. while, the DNA triangular prism cannot be formed in the lack of target. Thus, a very weak fluorescence can be measured after addition of PG. The proposed biosensor indicated high selectivity, a broad linear range from 200 pg/mL to 300 ng/mL and a very low detection limit of 30 pg/mL for PSA. Applying the designed aptasensor, PSA was successfully detected in human serum samples. This work provides a new way for detection of biomarkers in clinical samples.

Targeting inhibitors of apoptosis proteins (IAPs) family comprising high level expression in many cancer cells, could sensitize tumor cells to conventional chemotherapies. In the present study, we designed both doxorubicin and SmacN6 (an antagonist of the IAPs) encapsulated polymeric nanoparticles (NPs) and investigated their synergistic effect of combination therapy in vitro and in vivo. According to the results, NPs-SmacN6 significantly enhanced the cytotoxicity effect of NPs-DOX and reduced its IC50 in MCF-7, 4T1 and C26 cancer cells. Western blot analysis confirmed mechanism of cell apoptosis via caspase activation through intrinsic and also extrinsic pathways. Moreover, 5TR1 aptamer-modified NPs could effectively deliver DOX or SmacN6 to C26 cancer cells (MUC1 positive) in comparison with the non-targeted one (p

As one of the most toxic mycotoxins, aflatoxin B1 (AFB1) is a major food pollutant which can pose a high risk to human health. In this work, an accurate fluorescent sensing method was proposed for AFB1 determination, based on hairpin structure of G-quadruplex oligonucleotide-Aptamer chimera, silica nanoparticles coated with streptavidin (SNPs-Streptavidin) and N-methyl mesoporphyrin IX (NMM). The hairpin structure of chimera and SNPs-Streptavidin allowed AFB1 detection with high sensitivity and specificity. Moreover, the developed sensor could detect AFB1 in 30 min. The relative fluorescence intensity increased as AFB1 concentrations increased with a linear range of 30–900 pg/mL and a limit of detection (LOD) of 8 pg/mL. The constructed aptasensor was successfully employed to assess AFB1 spiked grape juice and human serum samples. The analytical recovery of AFB1 in the grape juice samples ranged from 95 to 106%, implying the great potential of the presented aptasensor in food product analysis.

Kanamycin is an aminoglycoside antibiotic that can be useful against both gram negative and positive bacteria. However, if its serum levels are not controlled properly, it can cause serious side effects like ototoxicity and nephrotoxicity. The aim of this study was to design a simple and rapid fluorescent aptasensor for detection of kanamycin, based on Aptamer/Complementary strand (dsDNA)-capped mesoporous silica nanoparticles (MSNs) and Rhodamine B as a fluorescent probe. The MSNs pores were filled with Rhodamine B and then gated with dsDNA. In the presence of kanamycin, the aptamer sequence was separated from its complementary strand (CS), so that, uncovered the pores and leading to leakage of Rhodamine B. Thus, a significant increase in the fluorescence intensity was observed. The relative fluorescence intensity showed a linearity range from 24.75 nM to 137.15 nM of kanamycin with a detection limit of 7.5 nM. The aptasensor also showed to be useful for detection of kanamycin in serum samples and was able to distinguish kanamycin from other antibiotics, resulting in a sensitive, rapid and inexpensive method for kanamycin detection.

Tuberculosis is a major global health problem caused by the bacterium Mycobacterium tuberculosis (Mtb) complex. According to WHO reports, 53 million TB patients died from 2000 to 2016. Therefore, early diagnosis of the disease is of great importance for global health care programs. The restrictions of traditional methods have encouraged the development of innovative methods for rapid, reliable, and cost-effective diagnosis of tuberculosis. In recent years, aptamer-based biosensors or aptasensors have drawn great attention to sensitive and accessible detection of tuberculosis. Aptamers are small short single-stranded molecules of DNA or RNA that fold to a unique form and bind to targets. Once combined with nanomaterials, nano-scale aptasensors provide powerful analytical platforms for diagnosing of tuberculosis. Various groups designed and studied aptamers specific for the whole cells of M. tuberculosis, mycobacterial proteins and IFN-γ for early diagnosis of TB. Advantages such as high specificity and strong affinity, potential for binding to a larger variety of targets, increased stability, lower costs of synthesis and storage requirements, and lower probability of contamination make aptasensors pivotal alternatives for future TB diagnostics. In recent years, the concept of SOMAmer has opened new horizons in high precision detection of tuberculosis biomarkers. This review article provides a description of the research progresses of aptamer-based and SOMAmer-based biosensors and nanobiosensors for the detection of tuberculosis.

In the present study, we fabricated an efficient, simple biomimetic scaffold to stimulate osteogenic differentiation of mesenchymal stem cells (MSCs). Electrospun poly L-lactic acid nanofibers were employed to mimic the nanofibrillar structure of bone proteins and coated with hydroxyapatite nanoparticles to simulate bone minerals. Thereafter, we regulated the release pattern of BMP-2 peptide through covalent attachment of an optimized liposomal formulation to the scaffold. The fabricated platform provided a sustained release profile of BMP-2 peptide up to 21 days while supporting cellular attachment and proliferation without cytotoxicity. In-vitro results confirmed the superiority of the scaffold containing liposomes through enhancement of growth and differentiation of MSCs. Ectopic bone formation model exhibited significant localized initiation of bone formation of liposome incorporated scaffold. Consequently, these findings demonstrated that our designed platform with modified release properties of BMP-2 peptide considerably promoted osteogenic differentiation of MSCs making it a unique candidate for bone regeneration therapeutics.

To efficiently deliver the chemotherapeutics to the tumor tissue and minimize the associated adverse effects, nucleolin targeted hybrid nanostructure based on hollow mesoporous silica nanoparticles (HMSNs) were fabricated. To provide the controlled, sustained drug release and enhance blood circulation, the surface of doxorubicin-encapsulated HMSNs were coated with acetylated carboxymethyl cellulose (Ac-CMC) and then covalently conjugated to AS1411 aptamer for guided drug delivery to nucleolin overexpressed cancerous cells. In vitro cellular uptake and cytotoxicity studies confirmed that AS1411 aptamer specifically targets nucleolin overexpressing MCF-7 and C26 cells. Moreover, the in vivo tumor inhibitory effect of AS1411 aptamer conjugated formulation demonstrated a superior therapeutic efficiency over non-targeted formulation and free doxorubicin. The current study might open a new insight to the development of targeted intelligent hybrid materials based on AcCMC-coated HMSNs with high loading capacity, smart characteristics and desirable anticancer potential.

In this study, a novel electrochemical aptasensor was developed for detection of AFM1 aflatoxin M1 (AFM1) based on the hairpin-shaped structure of AFM1 aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the presence and absence of AFM1 and also negatively charged AuNPs allowed detection of AFM1 with high sensitivity and selectivity. In the absence of the AFM1, the hairpin structure of the Apt was intact. So, a weak peak current was obtained. However, addition of AFM1 could disassemble the hairpin structure of the Apt. Thus, the CS-modified AuNPs came to close proximity of the surface of electrode and a strong current signal was recorded upon the addition of methylene blue as redox agent. The aptasensor allowed determination of AFM1 with a detection limit of 0.9 ng/L. Finally, the aptasensor was successfully applied for detection of AFM1 in real samples, including milk and serum samples.

Herein, a novel colorimetric aptasensor was introduced for detection of cocaine based on the formation of three-way junction pockets on the surfaces of gold nanoparticles (AuNPs) and the catalytic activity of the surfaces of AuNPs. Simplicity and detection of cocaine in a short time (only 35 min) are some of the unique features of the proposed sensing strategy. In the presence of cocaine, triple-fragment aptamer (TFA) forms on the surfaces of AuNPs, leading to a significant decrease of the catalytic activity of AuNPs and the color of samples remains yellow. In the absence of target, TFA does not form on the surfaces of AuNPs and 4-Nitrophenol, as a colorimetric agent, has more access to the surfaces of AuNPs, resulting in the reduction of 4-Nitrophenol and the color of sample changes from yellow to colorless. The sensing strategy showed good specificity, a limit of detection (LOD) of 440 pM and a dynamic range over 2–100 nM. The sensing method was also successfully applied to detect cocaine in spiked human serum samples with recovery of 94.71–98.63%.

Vascular endothelial growth factor (VEGF) is a key regulator of vascular formation and a predominant protein biomarker in cancer angiogenesis. Owing to its crucial roles in the cancer metastasis, VEGF detection and quantification is of great importance in clinical diagnostics. Today, there exist a wide variety of detection strategies for identifying many types of disease biomarkers, especially for VEGF. As artificial single-stranded DNA or RNA oligonucleotides with catalytic and receptor properties, aptamers have drawn lots of attention to be applied in biosensing platforms due to their target-induced conformational changes as well as high stability and target versatility. So far, various sensitivity-enhancement techniques in combination with a broad range of smart nanomaterials have integrated into the design of novel aptasensors to improve detection limit and sensitivity of analyte detection. This review article provides a brief classification and description of the research progresses of aptamer-based biosensors and nanobiosensors for the detection and quantitative determination of VEGF based on optical and electrochemical platforms.

To protect human health from the side effects of acrylamide in heat-processed food samples, simple analytical approaches are highly desired to determine low concentrations of acrylamide. In this study, a simple, rapid and accurate fluorescent sensor was developed for detection of acrylamide based on gold nanoparticles (AuNPs) and FAM-labeled double-stranded DNA (FAM-dsDNA). The sensing method was developed in a way to produce a remarkable fluorescence intensity difference in the absence and presence of acrylamide. In the presence of acrylamide, the single-stranded DNA (ssDNA) and acrylamide adduct is formed. So that, FAM-labeled complementary strand DNA (FAM-csDNA) is free in the environment and adsorbed on the surface of AuNPs and as a result, the FAM is quenched by the AuNPs. Under optimized conditions, the presented fluorescent analytical approach showed high selectivity toward acrylamide with a wide linear response, 1 × 10−7 M–0.05 M, and a limit of detection (LOD) of 1 × 10−8 M for acrylamide. This new method indicated excellent analytical performance for acrylamide detection in potato fries water extract samples with LOD of 0.5 × 10−6 M.

Triggering unpredictable allergic reactions associated with the presence of hidden allergens in the food chains have raised the need for precise, fast and reliable analytical methods to identify food allergens in different sources. Unintentional cross contamination of food products with allergen sources during production, transformation or processing is also an important risk factor for triggering allergenic reactions in suspected individuals. Different types of antibody based detection methods are applied for detection and standardization of food-born allergens. Aptamers attracted a great attention because of their small size, low cast and ease of manufacturing. Several detection strategies have been investigated for food allergen aptasensing such as electrochemical, optical, thermometric and piezoelectric. In this review, advances and the future trends of developed aptasensors for the control and detection of food-born allergens have been discussed.

New integrin-targeted nanoparticles made of chitosan-stabilized PLGA matrix was developed to specifically target colon adenocarcinoma. To this aim, SN38-encapsulated chitosan-coated PLGA NPs were conjugated with tetrac for integrin receptor-guided delivery. To provide a sustained release pattern for SN38, it was loaded into nanoparticles using single emulsion method. The size of NPs were 174.23 ± 6.12 nm with drug encapsulation efficiency and loading content of 73.16 ± 11.15 and 4.45 ± 0.31, respectively. The in vitro results confirmed that the designed nanoplatform showed specific cellular uptake and cytotoxicity in integrin overexpressing cancer cells and provided a sustained release profile for SN38. Additionally, an increased therapeutic potency of targeted formulation over both non-targeted and free drug was shown in vivo.

Targeted delivery of DNA nanoparticles is a promising approach in cancer therapy. Using aptamers, target specific delivery of DNA nanoparticles can be achieved. Further, aptamers can indirectly improve drug encapsulation efficiency of DNA nanoparticles for drugs intercalated within nucleic acid base pairs. Using DNA blocks, a micellar hybrid nanoparticle was prepared for the targeted co-delivery of doxorubicin and a pro-apoptotic peptide, KLA to tumor cells. Results demonstrated that anti-MUC1 aptamer could specifically deliver the synthesized DNA micelle into MCF-7 cells by improving its cellular uptake. Additionally, co-delivery of doxorubicin and KLA could significantly enhance the therapeutic efficacy of the construct resulting in reduction of required dose of doxorubicin that is a pivotal point in reducing chemotherapeutics side effects. Moreover, DOX-KLA-anti-MUC1-micelle remarkably inhibited tumor growth of tumor-bearing mice when compared with free drug. DOX-KLA-anti-MUC1-micelle also reduced toxic effect of free doxorubicin as determined by percent of body weight loss and survival rate in vivo.

Background Aptasensors are promising biosensors with prominent recognition capabilities. They have fascinated a lot of attention among scholars, due to the excellent characteristics of aptamers in combination with the use of nanostructures and new interface materials. The high sensitivity and selectivity of such platforms provide a promising view in food analysis. Scope and approach The uncontrolled usage of antibiotics, such as tetracyclines (TCs), results in the accumulation of antibiotics in food products. The traditional analytical method for detection of antimicrobial residues in food products is liquid chromatography coupled to mass spectrometric detection. Today, simple, sensitive and rapid schemes are needed for an on-site screening analysis. However, the routine techniques for TCs detection are not designed for this purpose. This review summarizes electrochemical and optical tetracycline aptasensors in food and buffer samples with focusing on modern methods and recent advances on aptamer-based tetracycline detection methods. Key findings and conclusions Here, we discussed several optical and electrochemical transduction systems and their principles in aptasensor-based tetracycline detection for the first time and we focused on modern methods and recent advances. Although an optical biosensor will always have the advantage of being easier to operate with inexpensive instrument, but electrochemical aptasensors offer higher sensitivity, repeatability and accuracy. Finally, we address current challenges and future directions.

Simple and reliable sensors for detection of arsenic (As), a toxic heavy metal, in drinking water and blood serum are highly desirable. Herein, we reported the fabrication and characterization of a fluorescent aptasensor for the detection of As (III) using target-induced conformational change of the Biotin and FAM-labeled complementary strand of aptamer (CS1), silica nanoparticles coated with streptavidin (SNPs-Streptavidin) and a label-free aptamer (Apt). The sensor described here had several attractive features, such as simplicity, rapid response and use of label-free Apt. Upon addition of As (III), Apt released its CSs and CS1 formed a hairpin structure on the surface of SNPs-Streptavidin, leading to a strong fluorescence signal. Without introduction of As (III), Apt was hybridized with its CSs and a weak fluorescence signal was obtained. The sensor exhibited a wide linear range between 2 and 500 nM and a very low detection limit of 0.45 nM. In addition, the applicability of the developed method was tested using spiked tap water and serum samples with satisfactory results.

Herein, we report a novel approach to design a fluorescent aptasensor for detection of carcinoembryonic antigen (CEA), a tumor marker, using three-way junction pocket and 5,6,7-trimethyl-1,8-naphthyridin-2-amine (ATMND) as a fluorescent agent. The analytical method was based on the entrapment of ATMND in the three-way junction pocket in the absence of CEA, leading to a significant decrease of fluorescence intensity of ATMND. Under, the optimum condition, the relative fluorescence intensity indicated a wide linearity range from 4.5 pg/mL to 30 ng/mL of CEA with a detection limit of 1.5 pg/mL. The aptasensor was also successfully used in human serum for CEA detection, which showed a good recovery (96.1–106.7%). This aptasensor owned several advantages such as simplicity and application of a label-free aptamer.

In regard to tremendous toxicological effects of aflatoxin B1 (AFB1) and its major metabolite aflatoxin M1 (AFM1) on human health, quality assessment of food is of great importance. On the other hand, due to extremely low levels of these toxic metabolites in food and feed, the need to develop reliable and sensitive method is inevitable. Although, very different analytical techniques have been introduced for detection of them, hindrances such as time-consuming, labor-intensive, and highly-priced instruments have limited their uses. Aptasensors, as emerging methods, have opened new horizons for sensitive and selective detection of toxins. Aptamers are a new class of characteristic bio-recognition elements, provided by an in vitro process named SELEX (Systematic Evolution of Ligands by EXponential enrichments). This review discusses aptasensors developed for detection of AFB1 and AFM1. Additionally, we aimed to investigate the advantages and disadvantages of these systems and introduce the best ones.

Herein, a sensitive and rapid fluorescent aptasensor was developed for the detection of cocaine as an illicit drug, based on an amplification strategy involving the use of silica nanoparticles coated with streptavidin (SNPs), double quenching of FAM fluorophore and cocaine split aptamer. Presence of cocaine could trigger the formation of the double-fragment cocaine aptamer and bring TAMRA fluorophore near the SNP surface and FAM close to BHQ-1 and TAMRA, resulting in the enhancement of relative fluorescence signal (fluorescence intensity of TAMRA/fluorescence intensity of FAM). Under optimal conditions, the proposed aptasensor achieved a limit of detection (LOD) of 84 pM and a linear range of 500 pM to 80 nM for cocaine. The practical potential of the developed sensing platform was proved by the detection of cocaine in spiked human serum samples with satisfactory recoveries.

Tramadol hydrochloride (TH), as an atypical opioid and a 4-phenyl-piperidine analogue of codeine, is mainly used for treating moderate to severe pains. Due to its extensive application, the consequent need for its analysis in various samples is essential. The current study focuses on the introduction of a rapid fluorescent assay using graphene oxide (GO) and aptamer for determination of tramadol in serum samples. Specific ssDNA aptamers for TH were developed by SELEX (Systematic Evolution of Ligands by EXponential Enrichment) technique using GO as a fluorescence quencher. After 10 rounds, two aptamers (Apt19 and Apt39) were selected from various families. Then, the binding constants of aptamers were measured using fluorometric assay and finally Apt39 (labeled with ATTO 647N) was chosen for development of a fluorescent aptasensor because this aptamer bound to TH with high affinity (Kd = 178.4 nM) and specificity. The current analytical system showed detection limits of 1.04 nM and 2.56 nM in serum sample and phosphate buffer saline (10 mM PBS), respectively.

Herein, a novel targeted delivery system was developed for intracellular co-delivery of doxorubicin (DOX) as a chemotherapeutic drug, antimiR-21 as an oncogenic antagomiR. In this system, DOX was loaded into mesoporous silica nanoparticles (MSNs) and chitosan was applied to cover the surface of MSNs. AS1411 aptamer as targeting nucleolin and antimiR-21 were electrostatically attached onto the surface of the chitosan-coated MSNs and formed the final nanocomplex (AACS nanocomplex). The study of drug release was based on DOX release under pH 7.4 and 5.5. Cellular toxicity and cellular uptake assessments of AACS nanocomplex were carried out in nucleolin positive (C26, MCF-7, and 4T1) and nucleolin negative (CHO) cell lines using MTT assay and flow cytometry analysis, respectively. Also, Anti-tumor efficacy of AACS nanocomplex was evaluated in C26 tumor-bearing mice. Overall, the results show that the combination therapy of DOX and antimiR-21, using AACS nanocomplex, could combat the cancer cell growth rate.

Employing targeting ligands with high affinity to tumour receptors is an important strategy to increase treatment efficacy. The use of aptamers as targeting agent is increasingly prevalent in drug delivery systems. Mucin1 (MUC1) is a glycoprotein that is over-expressed on the surface of several cancer cells and plays an important role in metastasis and invasion. 5TR1-aptamer is a DNA aptamer, which targets MUC1 receptors. The present study investigated the anti-tumour activity and therapeutic effectiveness of 5TR1-aptamer-PEGylated liposomal doxorubicin (PLD) delivery system in C26 tumour-bearing mice. The in vitro experiments demonstrated enhanced cytotoxicity and cellular uptake of PLD at the presence of 5TR1 aptamer into MUC1+C26 cell line. Biodistribution study indicated that aptamer conjugation increased tumour accumulation of PLDs. Pharmacokinetic analysis showed despite higher clearance rate, selective delivery of doxorubicin to tumour tissue was increased in the 5TR1-Doxil group. In C26-bearing tumour mice, treatment with 5TR1-Doxil exhibited significant deceleration in tumour growth and enhanced survival. The results suggested that 5TR1 aptamer is promising ligand for active targeting which improves therapeutic efficiency of PLD in cancer therapy.

Helicobacter pylori is a species of bacteria that can colonize the human stomach mucosa. It is closely associated with gastric diseases. The restrictions of traditional methods have encouraged the development of innovative methods for rapid, reliable, and cost-effective diagnosis of H. pylori infection. In recent years, the concept of biosensor and microfluidic-based devices has opened new horizons in high-precision detection. Once combined with nanomaterials, nano-scale biosensors and microfluidic systems provide powerful analytical platforms for point of care (POC) diagnosing of H. pylori. In this article, a brief overview of general aspects of H. pylori infection and current diagnostic methods are firstly discussed. In addition, a clear and concise review of recent advances of biosensors, paper-based and microfluidic systems based on nanomaterials for the detection of H. pylori are discussed herein. Subsequently, the latest development of integrated and miniaturized microfluidic biosensing technologies for POC detection of H. pylori is explained.

Due to the severe cardiotoxicity of doxorubicin, its usage is limited. This shortcoming could be overcome by modifying pharmacokinetics of the drugs via preparation of various nanoplatforms. Doxil, a well-known FDA-approved nanoplatform of doxorubicin as antineoplastic agent, is frequently used in clinics in order to reduce cardiotoxicity of doxorubicin. Since Doxil shows some shortcomings in clinics including hand and food syndrome and very slow release pattern thus, there is a demand for the development and preparation of new doxorubicin nanoformulation with fewer side effects. The new formulation of the doxorubicin, synthesized previously by our group was extensively examined in the current study. This new formulation is doxorubicin encapsulated in PEG-PLGA polymersomes (PolyDOX). The main aim of the study was to compare the distribution and treatment efficacy of a new doxorubicin-polymersomal formulation (PolyDOX) with regular liposomal formulation (Doxil-mimic) in murine colon adenocarcinoma model. Additionally, the pathological, hematological changes, pharmacodynamics, biodistribution, tolerated dose and survival rate in vivo were evaluated and compared. Murine colon cancer model was induced by subcutaneous inoculation of BALB/c mice with C26 cells. Afterwards, either Doxil-mimic or PolyDOX was administered intravenously. The obtained results from biodistribution study showed a remarkable difference in the distribution of drugs in murine organs. In this regard, Doxil-mimic exhibited prolonged (48h) presence within liver tissues while PolyDOX preferentially accumulate in tumor and the presence in liver 48h post-treatment was significantly lower than that of Doxil-mimic. Obtained results demonstrated comparable final length of life for mice receiving either Doxil-mimic or PolyDOX formulations whereas tolerated dose of mice receiving Doxil-mimic was remarkably higher than those receiving PolyDOX. Therapeutic efficacy of formulation in term of tumor growth rate after one injection of formulations (5mg/kg, 10mg/kg or 15mg/kg) demonstrated better efficacy at lower dose for PolyDOX. Analysis of Kaplan Meier curve was in favor of both formulations in their treatment-dose. Pathological and hematological surveys of mice treated with both formulations did not show considerable difference except for a small atrophy in liver observed after successive administration of Doxil-mimic. It could be concluded that PolyDOX can potentially limit off-site effects of Doxil due to its biodegradability and sustained release properties while it exhibited favorable safety profile comparable to Doxil.

Non-viral vectors have attracted great interest, as they are simple to prepare, easy to modify and relatively safe, compared to viral vectors. Kidney-targeted gene delivery systems depict a promising technology to improve drug efficacy in renal diseases treatments. In order to develop a novel kidney-targeted gene delivery system, we synthesized polyamine-PEI conjugates using polymyxin B as ligand and investigated their potential targeting efficiency. After grafting either PEI25 kDa or PEI10 kDa with polymyxin B through amidation reaction, the modified-polymyxin-PEI/DNA-nanoplexes were produced via electrostatic attraction between the cationic polymers and EGFP plasmid. The properties of modified polymers including size, surface charge density, DNA condensation ability, buffering capacity and cytotoxicity were evaluated. Results revealed that the average size of -modified-polymyxin- PEI25kDa was about 143-180nm and modified-polymyxin-PEI10kDa 115-194nm. The zeta potentials were in the range of 16.4±1.87 to 23.43±1.25mV and 11.3±1.4 to 19.3±2.1mV for conjugates based on PEI25 and PEI10 respectively. The AFM images revealed that the complexes were spherical and nano-sized at C/P=4. The buffering capacity of both PEI 10 and 25kDa increased as the percentage of polymyxin B grafting increased. In vitro study demonstrated that modified-polymyxin-PEI conjugates could remarkably improve the gene transfection efficiency to kidney cells. The transfection efficiency of the polyplexes was dependent on the weight ratio of ligand in the formulation (~12 and 8 fold increase for PEI25 and PEI10kDa, respectively) and was significantly higher than that of unmodified PEIs/DNA nanoparticles. These results suggest that modified-polymyxin-PEI /DNA nanoparticles can effectively target megalin-expressing kidney cells and show improved transfection efficiency and low cytotoxicity in In vitro and In vivo studies. Animal studies confirmed the in vivo study. Thus, these conjugates can be considered as a safe and efficient non-viral therapeutic therapy vector for kidney diseases.

Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337 µM with LOD of 0.025 µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6 µg/µL at 260 nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280 nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5 ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1 ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%.

In this study, we prepared tetraiodothyroacetic acid (tetrac) conjugated PEG-PLGA polymersomes for the targeted delivery of camptothecin to colon adenocarcinoma. Tetrac, which binds to integrin αvβ3 with high affinity and specificity, was covalently conjugated to the surface of the PEGylated polymersomal formulation of camptothecin (CPT). The hydrodynamic and morphological properties of the prepared system were evaluated using TEM (transmission electron microscopy), SEM (scanning electron microscopy) and DLS (dynamic light scattering) experiments. Camptothecin was encapsulated in the polymersomal system with encapsulation efficiency and loading content of 84 ± 10.12 and 4.2 ± 0.82, respectively. The in vitro release profile of camptothecin from the polymersomal formulation revealed the sustained release pattern. In vitro cytotoxicity experiments confirmed that the tetrac-conjugated camptothecin loaded-polymersomes had higher cellular toxicity towards integrin-overexpressed HT29 and C26 colorectal cancer cells than integrin-negative CHO cell line. The in vivo tumor inhibitory effect of tetrac-conjugated camptothecin loaded-polymersomes demonstrated an enhanced therapeutic index of integrin targeted polymersomal formulation over both non-targeted polymersomal formulation and free camptothecin in C26 tumor bearing mice. The obtained results demonstrated that the prepared tetrac-conjugated polymersomes were able to control the release of camptothecin, and significantly increase the therapeutic index of compthotecin. This study demonstrates the versatility of integrin-targeted tetrac-conjugated PEG-PLGA polymersomal formulation as an anti-cancer nano-pharmaceutical platform.

There is a clinical need for a novel, more efficient therapy for full thickness wound healing. In the current study, curcumin encapsulated PEG-PLA [poly(lactide)-block-poly(ethylene glycol)] nanomicelles were incorporated into dextran hydrogel for a full thickness dermal wound healing application. To assess the application of the hydrogel as a therapeutic wound dressing, its morphology, swelling pattern, kinetics of degradation, and capacity to control curcumin release were evaluated. It was found that the prepared hybrid hydrogel had acceptable biocompatibility, incorporation capacity of curcumin nanomicelles, and mechanical properties. An in vitro release experiment also demonstrated the sustained release of curcumin from dextran hydrogel, which was first controlled by the diffusion of curcumin from hydrogel and continued through hydrogel matrix erosion at the terminal phase. An in vivo wound healing experiment was carried out using dressing hydrogels on full thickness wounds in BALB/c mice. An histological study demonstrated that the application of curcumin nanomicelles incorporated hydrogel could significantly augment the re-epithelialization of epidermis and collagen deposition in the wound area. Expression of CD31 and vimentin in wound tissue was investigated using immunohistochemistry tests on the eighth day post wounding. The results obtained demonstrated that curcumin nanomicelles incorporated hydrogel could significantly accelerate angiogenesis, fibroblast accumulation, and the process of wound healing. Together, the data indicate that the prepared hybrid curcumin PEG-PLA nanomicelles incorporated dextran hydrogel is a promising candidate for full thickness wound treatment that increases re-epithelialization, collagen deposition, angiogenesis, and tissue granulation.

Cancer is a broad term for a class of prevalent diseases as one in three people develop cancer during their lifetime. Although, there are few success stories of cancer therapy, most of the existing medications do not lead to complete recovery. Because of the complexity of cancer, usually a single therapeutic approach is insufficient for the suppression of cancer growth and metastasis. Simultaneous loading and co-delivery of different agents with different physiochemical characteristics to the same tumors have been suggested for minimizing the dose of anticancer drugs and achieving the synergistic therapeutic impacts in cancers treatment. Intense work to develop nanotechnology-based systems as a suitable option for cancer treatment is currently underway. The purpose of this review is to provide an overview of the co-delivery systems based on polymeric nanoparticles including polymeric micelles, dendrimers, poly-d,l-lactide-co-glycolide, polyethylenimine, poly(l-lysine) and chitosan for efficacious cancer therapy.

The authors describe an aptamer-based colorimetric assay for the specific detection of the toxic microcystin leucine-arginine (MC-LR). The method is based on the use of graphene oxide (GO) in a hydrogel matrix, Methylene Blue (MB) acting as an optical probe, streptavidin-modified magnetic particles, and an aptamer (Apt) as a the recognition probe. A complementary strand of the aptamer and adenosine act as promoters in the formation of the GO hydrogel, and the hairpin structure of the aptamer warrants selectivity. In the absence of MC-LR, the gelation of GO sheets is promoted by complementary strand and adenosine, and MB is trapped between GO sheets. Hence, no blue color can be observed in the sample solution. In the presence of MC-LR, GO does not cause the formation of a strong hydrogel structure and a distinct blue color can be observed and measured (at 660 nm) because MB is present in solution. The assay has high selectivity for MC-LR, with a limit of detection (LOD) as low as 219 pM. It was successfully applied to the determination of MC-LR in spiked tap water and serum samples where it gave LODs of 221 and 412 pM, respectively.

Recently carbon nanotubes (CNTs) showed promising potentials in different biomedical applications but their safe use in humans and probable toxicities are still challenging. The aim of this study was to determine the acute toxicity of functionalized single walled carbon nanotubes (SWCNTs). In this project, PEGylated and Tween functionalized SWCNTs were prepared. BALB/c mice were randomly divided into nine groups, including PEGylated SWCNTs (75,150μg/mouse) and PEG, Tween80 suspended SWCNTs, Tween 80 and a control group (intact mice). One or 7 days after intravenous injection, the mice were killed and serum and livers were collected. The oxidative stress markers, biochemical and histopathological changes were studied. Subsequently, proteomics approach was used to investigate the alterations of protein expression profiles in the liver. Results showed that there were not any significant differences in malondealdehyde (MDA), glutathione (GSH) levels and biochemical enzymes (ALT and AST) between groups, while the histopathological observations of livers showed some injuries. The results of proteomics analysis revealed indolethylamine N-Methyltransferase (INMT), glycine N-Methyltransferase (GNMT), selenium binding protein (Selenbp), thioredoxin peroxidase (TPx), TNF receptor associated protein 1(Trap1), peroxiredoxin-6 (Prdx6), electron transport flavoprotein (Etf-α), regucalcin (Rgn) and ATP5b proteins were differentially expressed in functionalized SWCNTs groups. Western blot analyses confirmed that the changes in Prdx6 were consistent with 2-DE gel analysis. In summary, acute toxicological study on two functionalized SWCNTs did not show any significant toxicity at selected doses. Proteomics analysis also showed that following exposure to functionalized SWCNTs, the expression of some proteins with antioxidant activity and detoxifying properties were increased in liver tissue.