1. Interaction of serum proteins with SARS-CoV-2 RBD
- Author
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Hong-ming Ding, Yue-Wen Yin, Yan-Jing Sheng, Min Wang, and Yu-qiang Ma
- Subjects
0301 basic medicine ,Apolipoprotein E ,Coronavirus disease 2019 (COVID-19) ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Protein Corona ,02 engineering and technology ,Plasma protein binding ,03 medical and health sciences ,medicine ,Humans ,General Materials Science ,skin and connective tissue diseases ,biology ,SARS-CoV-2 ,fungi ,virus diseases ,COVID-19 ,Blood Proteins ,021001 nanoscience & nanotechnology ,Human serum albumin ,Blood proteins ,body regions ,Molecular Docking Simulation ,030104 developmental biology ,Immunology ,Spike Glycoprotein, Coronavirus ,biology.protein ,Antibody ,0210 nano-technology ,medicine.drug ,Protein Binding - Abstract
The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has become a worldwide public health crisis. When the SARS-CoV-2 enters the biological fluids in the human body, different types of biomolecules (in particular proteins) may adsorb on its surface and alter its infection ability. Although great efforts have recently been devoted to the interaction of specific antibodies with the SARS-CoV-2, it still remains largely unknown how the other serum proteins affect the infection of the SARS-CoV-2. In this work, we systematically investigate the interaction of serum proteins with the SARS-CoV-2 RBD by molecular docking and all-atom molecular dynamics simulations. It is found that non-specific immunoglobulins (Ig) indeed cannot effectively bind to the SARS-CoV-2 RBD while human serum albumin (HSA) may have some potential in blocking its infection (to ACE2). More importantly, we find that the RBD can cause significant structural changes in Apolipoprotein E (ApoE), by which SARS-CoV-2 may hijack the metabolic pathway of ApoE to facilitate its cell entry. The present study enhances the understanding of the role of protein corona in the bio-behaviors of SARS-CoV-2, which may aid the more precise and personalized treatment for COVID-19 infection in the clinic.
- Published
- 2021