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18 results on '"Roxana Jijie"'

1. Particle-based photodynamic therapy based on indocyanine green modified plasmonic nanostructures for inactivation of a Crohn's disease-associated Escherichia coli strain

Author

Li Chengnan, Julie Bouckaert, N. Dumitrascu, Benoit Cudennec, Rabah Boukherroub, Tetiana Dumych, Kostiantyn Turcheniuk, Roxana Jijie, Sabine Szunerits, Laurent Héliot, Charles-Henri Hage, Alexandru Ioan Cuza University of Iași [Romania], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut Charles Viollette (ICV) - EA 7394 (ICV), and Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille

Subjects
Materials science, medicine.medical_treatment, Biomedical Engineering, Nanoparticle, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, Photochemistry, medicine.disease_cause, 01 natural sciences, chemistry.chemical_compound, medicine, General Materials Science, Photosensitizer, Escherichia coli, Singlet oxygen, General Chemistry, General Medicine, Photothermal therapy, 021001 nanoscience & nanotechnology, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, chemistry, Nanorod, 0210 nano-technology, Indocyanine green
Abstract

Particle-based photodynamic therapy (PPDT) holds great promise in theranostic applications. Herein, we demonstrate that PPDT based on gold nanorods coated with an indocyanine green (ICG)-loaded silica shell allows for the inactivation of the Crohn's disease-associated adherent-invasive Escherichia coli strain LF82 (E. coli LF82) under pulsed laser light irradiation at 810 nm. Fine-tuning of the plasmonic structures together with maximizing the photosensitizer loading onto the nanostructures allowed optimizing the singlet oxygen generation capability and the PPDT efficiency. Using a nanoparticle concentration low enough to suppress photothermal heating effects, 6 log10 reduction in E. coli LF82 viability could be achieved using gold nanostructures displaying a plasmonic band at 900 nm. An additional modality of nanoparticle-based photoinactivation of E. coli is partly observed, with 3 log10 reduction of bacterial viability using Au NRs@SiO2 without ICG, due to the two-photon induced formation of reactive oxygen species. Interaction of the particles with the bacterial surface, responsible for the disruption of the bacterial integrity, together with the generation of moderate quantities of singlet oxygen could account for this behavior.

Published
2020

2. Enhanced antibacterial activity of carbon dots functionalized with ampicillin combined with visible light triggered photodynamic effects

Author

Julie Bouckaert, N. Dumitrascu, Rabah Boukherroub, Roxana Jijie, Alexandre Barras, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)

Subjects
Adult, Light, Cell Survival, chemistry.chemical_element, Microbial Sensitivity Tests, 02 engineering and technology, Bactericidal activity, Conjugated system, 010402 general chemistry, 01 natural sciences, Nanomaterials, Structure-Activity Relationship, Colloid and Surface Chemistry, PDT, Ampicillin, Quantum Dots, Escherichia coli, Tumor Cells, Cultured, medicine, Carbon dots, [CHIM]Chemical Sciences, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amines, Physical and Theoretical Chemistry, Cell Death, Dose-Response Relationship, Drug, Escherichia coli K12, [CHIM.MATE]Chemical Sciences/Material chemistry, Surfaces and Interfaces, General Medicine, 021001 nanoscience & nanotechnology, Combinatorial chemistry, Carbon, Anti-Bacterial Agents, 0104 chemical sciences, Photochemotherapy, chemistry, Female, 0210 nano-technology, Antibacterial activity, HeLa Cells, Biotechnology, Visible spectrum, medicine.drug
Abstract

International audience; In the last years, carbon-based nanomaterials have attracted considerable attention in a wide range of fields, particularly in biomedicine, owing to their remarkable photo-physical and chemical properties. In this study, we demonstrate that amine-terminated carbon dots (CDs-NH2) functionalized with ampicillin (AMP) offer a new perspective for antibacterial treatment. The amine-functionalized carbon dots were used as a carrier for immobilization and delivery of ampicillin (CDs-AMP) and as a visible light-triggered antibacterial material. Additionally, AMP immobilization on the CDs-NH2 surface improves its stability in solution as compared to free AMP. The AMP conjugated CDs platform combines the antibacterial function of AMP and conserves the intrinsic theranostic properties of CDs-NH2. Therefore, the AMP immobilized onto CDs-NH2 surface together with the generation of moderate quantities of reactive oxygen species under visible light illumination is very effective to inactivate the growth of Escherichia coli.

Published
2018

3. Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 2: Application to the analysis of calcitonin containing pharmaceutical formulation

Author

Sreekumar Kurungot, Szilveszter Gáspár, Mohamed Salah Medjram, Sabrina Lamraoui, Rabah Boukherroub, Sorin Melinte, Alina Vasilescu, Santosh K. Singh, Roxana Jijie, Ran Ye, Sabine Szunerits, Samia Boulahneche, International Centre of Biodynamics, Université Catholique de Louvain = Catholic University of Louvain (UCL), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratoire de Génie Chimique et Environnement, Université 20 Août 1955 Skikda, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), and UCL - SST/ICTM/ELEN - Pôle en ingénierie électrique

Subjects
Porous reduced graphene oxide, General Chemical Engineering, Oxide, Peptide, 02 engineering and technology, Protein aggregation, Pharmaceutical formulation, 010402 general chemistry, Electrochemistry, 01 natural sciences, law.invention, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, law, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Graphene, Calcitonin, Disposable electrodes, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Chemical engineering, Differential pulse voltammetry, Lysozyme, 0210 nano-technology
Abstract

In part 1 (A. Vasilescu et al., Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 1: Lysozyme aggregation at pH 2 and 7.4 Electrochem. Acta, 254 (2017) 375–383) we proposed porous reduced graphene oxide coated glassy carbon electrode (GC/prGO) in combination with differential pulse voltammetry as a new analytical tool for aggregation studies of proteins. Lysozyme was used as a model to follow its aggregation by electrochemical means at pH 2 and pH 7.4, leading to the formation of amyloid and amorphous aggregates, respectively. Part 2 of this work widens the scope of this approach by investigating a biopharmaceutical product, as the aggregation of peptide based drugs affects their therapeutic activity and can induce allergic reactions in patients. The salmon polypeptide calcitonin (sCT) was chosen as an example of a bioactive peptide with limited pharmaceutical potential due to a tendency to form cytotoxic aggregates and amyloid fibrils. For practical applications, screen printed electrodes (SPE) and flexible electrodes (FE) modified with polydiallyldimethylammonium (PDDA) and prGO by using the layer-by-layer deposition technique have been developed for the detection of sCT. The results indicate that these electrodes can differentiate between formation of amyloid aggregates of calcitonin (2 mg mL−1) in citrate buffer to no aggregation in acetate buffer. It was further demonstrated that these electrodes are able to analyze a pharmaceutical drug product of low potency, Miacalcic (8.3 μg mL−1), where no aggregation was observed.

Published
2018

4. Advancements on the molecular design of nanoantibiotics: current level of development and future challenges

Author

Alexandre Barras, Rabah Boukherroub, Florina Teodorescu, Roxana Jijie, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institute of physical Chemistry 'llie Murgulescu', and European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016)

Subjects
Drug, medicine.drug_class, media_common.quotation_subject, Antibiotics, Biomedical Engineering, Energy Engineering and Power Technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 02 engineering and technology, Pharmacology, 010402 general chemistry, 01 natural sciences, Industrial and Manufacturing Engineering, Gastrointestinal complications, Pharmacokinetics, Oral administration, Materials Chemistry, medicine, Chemical Engineering (miscellaneous), media_common, business.industry, Process Chemistry and Technology, 021001 nanoscience & nanotechnology, Antimicrobial, 3. Good health, 0104 chemical sciences, Chemistry (miscellaneous), Active compound, Drug delivery, 0210 nano-technology, business
Abstract

International audience; Numerous antimicrobial drugs have been developed and commercialized to kill and inhibit the growth of pathogenic microbes. The therapeutic efficiency of these drugs has however become often inadequate for the treatment of microbial infections, the reasons being multiple. Oral administration results often in only partial absorption and up to 50% of the active compound can be excreted in the urine. Furthermore, antibiotic therapy is also frequently accompanied by gastrointestinal complications, including vomiting, nausea and diarrhea. From a therapeutic point of view, the low solubility of antibiotics in lipid membranes presents a limitation for rapid and efficient treatment of infections. The preferred route for the delivery of antimicrobial drugs is the oral one; oral formulations capable of extending the duration of drug delivery and minimizing side effects have received much attention. The combination of antimicrobial agents with nanomaterials has been seen as a particularly promising strategy to overcome these limitations. Particulate formulations have proven their efficiency in achieving better pharmacokinetic profiles and improving the bio availability of several antibiotics. Antibiotic modified particles have shown their capability to protect some of the antimicrobial drugs from stomach acid and first-pass metabolisms in the gastrointestinal tract. Likewise, particle formulations can increase the circulation times of a drug and the local concentration gradient across absorptive cells. The present review describes the current status of different types of antibiotic loaded nano-systems. Key principles such as antibiotic loading, the release mechanism and the mode of action involved in pathogen killing or inhibition will be discussed in more detail. It is hoped that the general knowledge obtained here will help in the generation of advanced nanomaterials loaded with antimicrobials agents.

Published
2017

5. Formation of positive ions in hydrocarbon containing dielectric barrier discharge plasmas

Author

Roxana Jijie, A. V. Nastuta, Ionut Topala, Ioana Rusu, Valentin Pohoata, and Ilarion Mihaila

Subjects
Atmospheric Science, Materials science, Plasma cleaning, Hydrogen, Analytical chemistry, Aerospace Engineering, chemistry.chemical_element, Atmospheric-pressure plasma, 02 engineering and technology, Dielectric barrier discharge, 01 natural sciences, chemistry.chemical_compound, Propane, 0103 physical sciences, 010302 applied physics, chemistry.chemical_classification, Astronomy and Astrophysics, Butane, Plasma, 021001 nanoscience & nanotechnology, Geophysics, Hydrocarbon, chemistry, Space and Planetary Science, General Earth and Planetary Sciences, 0210 nano-technology
Abstract

Low temperature atmospheric pressure plasma devices are suitable experimental solutions to generate transitory molecular environments with various applications. In this study we present experimental results regarding the plasma chemistry of dielectric barrier discharges (DBD) in helium - hydrogen (0.1%) - hydrocarbons (1.2%) mixtures. Four types of hydrocarbon gases were studied: methane ( CH 4 ), ethane ( C 2 H 6 ), propane ( C 3 H 8 ), and butane ( C 4 H 10 ). Discharge diagnosis and monitoring was assured by electrical measurements and optical emission spectroscopy. Molecular beam mass spectrometry is engaged to sample positive ions populations from two different plasma sources. Dissociation and generation of higher-chain and cyclic (aromatic) hydrocarbons were discussed as a function of feed gas and discharge geometry. We found a strong influence of these parameters on both molecular mass distribution and recombination processes in the plasma volume.

Published
2016

6. Efficient capture and photothermal ablation of planktonic bacteria and biofilms using reduced graphene oxide–polyethyleneimine flexible nanoheaters

Author

Alexandre Barras, Alejandro Silhanek, Roxana Jijie, Sabine Szunerits, Ran Ye, Milica D. Budimir, Zoran Marković, Sorin Melinte, Rabah Boukherroub, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Université Catholique de Louvain = Catholic University of Louvain (UCL), Experimental Physics of Nanostructured Materials (Q-MAT, CESAM), Université de Liège, NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), UCL - SST/ICTM/ELEN - Pôle en ingénierie électrique, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Institut supérieur de l'électronique et du nunérique (ISEN)-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Université Catholique de Louvain (UCL)

Subjects
Materials science, Biomedical Engineering, Nanotechnology, macromolecular substances, 02 engineering and technology, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, law.invention, law, medicine, Humans, Polyethyleneimine, [CHIM]Chemical Sciences, General Materials Science, Absorption (electromagnetic radiation), Escherichia coli, ComputingMilieux_MISCELLANEOUS, Bacteria, biology, Graphene, Biofilm, Substrate (chemistry), General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, Photothermal therapy, Plankton, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, Kapton, Biofilms, Graphite, 0210 nano-technology
Abstract

Bacterial infections are one of the leading causes of disease worldwide. Conventional antibiotics are becoming less efficient, due to antibiotic-resistant bacterial strains. Therefore, the development of novel antibacterial materials and advanced treatment strategies are becoming increasingly important. In the present work, we developed a simple and efficient strategy for effective bacterial capture and their subsequent eradication through photothermal killing. The developed device consists of a flexible nanoheater, comprising a Kapton/Au nanoholes substrate, coated with reduced graphene oxide–polyethyleneimine (K/Au NH/rGO–PEI) thin films. The Au NH plasmonic structure was tailored to feature strong absorption in the near-infrared (NIR) region, where most biological matter has limited absorption, while PEI was investigated for its strong binding with bacteria through electrostatic interactions. The K/Au NH/rGO–PEI device was demonstrated to capture and eliminate effectively both planktonic Gram-positive Staphilococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria after 10 min of NIR (980 nm) irradiation and, to destroy and eradicate Staphilococcus epidermidis (S. epidermidis) biofilms after 30 min irradiation. The technique developed herein is simple and universal with potential applications for eradication of different micro-organisms.

Published
2019

7. NiFe layered double hydroxide electrodeposited on Ni foam coated with reduced graphene oxide for high-performance supercapacitors

Author

Rabah Boukherroub, Min Li, Pascal Roussel, Alexandre Barras, Roxana Jijie, Sabine Szunerits, IMEC (IMEC), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, and Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Supercapacitor, Materials science, Graphene, General Chemical Engineering, Oxide, 02 engineering and technology, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 7. Clean energy, Capacitance, Cathode, 0104 chemical sciences, Anode, law.invention, chemistry.chemical_compound, chemistry, Chemical engineering, law, Electrode, Electrochemistry, Hydroxide, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract

High-performance electrode materials consisting of intertwined caterpillar-like NiFe layered double hydroxide bridges coated on reduced graphene oxide modified Ni foam (NiFe LDHs/rGO/NF) were prepared via a facile two-step electrodeposition method. First, rGO was electrochemically coated onto NF at a constant potential of −1.2 V vs. SCE for 600 s. Then NiFe LDHs of different Ni to Fe ratios were electrodeposited onto the formed rGO/NF electrodes at −1.2 V vs. SCE for 10 s. Under optimized conditions, NiFe LDHs/rGO/NF composites displayed a high specific capacitance of 1462.5 F g−1 at a current density of 5 A g−1 and retained about 64.7% of the original capacitance after 2000 cycles. Furthermore, a flexible asymmetric supercapacitor was prepared using NiFe LDHs/rGO/NF as the cathode and mesoporous carbon (MC) coated on NF as the anode. The supercapacitor exhibited an energy density of 17.71 Wh kg−1 at a power density of 348.49 W kg−1. All of these findings suggest that the new electrodes developed in this work hold a potential application prospect in flexible energy storage devices.

Published
2019

8. Entrapment of uropathogenic E. coli cells into ultra-thin sol-gel matrices on gold thin films: A low cost alternative for impedimetric bacteria sensing

Author

Rabah Boukherroub, Esmat Abdi, Roxana Jijie, Sabine Szunerits, Mandana Amiri, Hamed Jafari, Julie Bouckaert, Saeid Latifi Navid, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Université de Lille, CNRS, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], University of Mohaghegh Ardabili, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), and Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Biomedical Engineering, Biophysics, Nanotechnology, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, Bacterial cell structure, Limit of Detection, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, Uropathogenic E. coli, Sol-gel, Thin film, Sensing, Electrochemical impedance spectroscopy, Humans, Uropathogenic Escherichia coli, [CHIM]Chemical Sciences, Electrodes, Escherichia coli Infections, ComputingMilieux_MISCELLANEOUS, Detection limit, biology, Chemistry, 010401 analytical chemistry, General Medicine, Silicon Dioxide, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, Dielectric spectroscopy, Linear range, Gold, 0210 nano-technology, Bacteria, Biotechnology
Abstract

International audience; Bacterial infections are causing worldwide morbidity and mortality. One way to limit infectious outbreaks and optimize clinical management of infections is through the development of fast and sensitive sensing of bacteria. Most sensing approaches are currently based on immunological detection principles. We report here on an impedimetric sensor to selectively and sensitive detect uropathogenic E. coli cells (E. coli UTI89) using artificial recognition sites. We show here the possibility to imprint the rod-shape structure of E. coli UTI 89 into ultra-thin inorganic silica coatings on gold electrodes in a reproducible manner. A linear range from to 1 × 10 0-1 × 10 4 cfu mL −1 is obtained. With a detection limit for E. coli UTI89 below 1 cfu mL −1 from five blank signals (95% confidence level) and excellent selective binding capabilities, these bacterial cell imprinted electrodes brings us closer to a low cost specific bacterial recognition surfaces.

Published
2019

9. Reduced Graphene-Oxide-Embedded Polymeric Nanofiber Mats: An 'On-Demand' Photothermally Triggered Antibiotic Release Platform

Author

Roxana Jijie, Julie Bouckaert, Solomiya Paryzhak, Sabine Szunerits, Volodymyr Vovk, Bianka Golba, Ismail Altinbasak, Rabah Boukherroub, Rostyslav Bilyy, Amitav Sanyal, Djamel Drider, Alexandre Barras, Rana Sanyal, Tetiana Dumych, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut Charles Viollette (ICV) - EA 7394 (ICV), Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille, Danylo Halytskiy Lviv National Medical University, Boğaziçi University [Istanbul], European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Boǧaziçi üniversitesi = Boğaziçi University [Istanbul], Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de la Recherche Agronomique (INRA)-Université d'Artois (UA)-Institut Supérieur d'Agriculture

Subjects
Male, Materials science, Oxide, antibiotic release, Acrylic Resins, Nanofibers, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, 01 natural sciences, reduced graphene oxide, law.invention, chemistry.chemical_compound, Mice, law, [CHIM]Chemical Sciences, Animals, General Materials Science, Irradiation, electrospinning, Acrylic acid, Mice, Inbred BALB C, Wound Healing, Graphene, Photothermal effect, [CHIM.MATE]Chemical Sciences/Material chemistry, Hyperthermia, Induced, Photothermal therapy, Phototherapy, 021001 nanoscience & nanotechnology, Electrospinning, 0104 chemical sciences, Anti-Bacterial Agents, chemistry, Chemical engineering, Nanofiber, Graphite, 0210 nano-technology, photothermal effect 35
Abstract

International audience; The steady increase in antimicrobial resistance in different 16 pathogens requires the development of alternative treatment strategies 17 next to the oral delivery of antibiotics. A photothermally activated 18 platform based on reduced graphene oxide (rGO)-embedded polymeric 19 nanofiber mats for on-demand release of antibiotics upon irradiation in 20 the near-infrared is fabricated. Cross-linked hydrophilic nanofibers, 21 obtained by electrospinning a mixture of poly(acrylic acid) (PAA) and 22 rGO, show excellent stability in aqueous media. Importantly, these PAA@ 23 rGO nanofiber mats exhibit controlled photothermal heating upon 24 irradiation at 980 nm. Nanofiber mats are efficiently loaded with 25 antibiotics through simple immersion into corresponding antibiotics 26 solutions. Whereas passive diffusion based release at room temperature is 27 extremely low, photothermal activation results in increased release within 28 few minutes, with release rates tunable through power density of the 29 applied irradiation. The large difference over passive and active release, as well as the controlled turn-on of release allows 30 regulation of the dosage of the antibiotics, as evidenced by the inhibition of planktonic bacteria growth. Treatment of superficial 31 skin infections with the antibiotic-loaded nanofiber mats show efficient wound healing of the infected site. Facile fabrication and 32 implementation of these photothermally active nanofiber mats makes this novel platform adaptable for on-demand delivery of 33 various therapeutic agents. 34

Published
2018

10. Solution Processable Cu(II)macrocycle for the Formation of Cu 2 O Thin Film on Indium Tin Oxide and Its Application for Water Oxidation

Author

Abolfazl Bezaatpour, Mandana Amiri, Maryam Fallahi, Rabah Boukherroub, Maryam Rouhi, Roxana Jijie, Sabine Szunerits, Mehran Nozari-asbmarz, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), and Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)

Subjects
Materials science, 02 engineering and technology, [CHIM.MATE]Chemical Sciences/Material chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, 7. Clean energy, 01 natural sciences, 6. Clean water, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Catalysis, Indium tin oxide, General Energy, Chemical engineering, Clean energy, Water splitting, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Thin film, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract

The development of efficient water oxidation catalytic interfaces has become a demanding challenge to fulfill the promise of water splitting for clean energy conversion and storage. The present wor...

Published
2018

11. Nucleic aptamer modified porous reduced graphene oxide/MoS2 based electrodes for viral detection: Application to human papillomavirus (HPV)

Author

Santosh K. Singh, Fereshteh Chekin, Komal Bagga, Rabah Boukherroub, Roxana Jijie, Sabine Szunerits, Palaniappan Subramanian, Sreekumar Kurungot, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé-Université de Lille, Sciences et Technologies, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), and Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS)

Subjects
Aptamer, Nanotechnology, 02 engineering and technology, Glassy carbon, 01 natural sciences, law.invention, Nanomaterials, chemistry.chemical_compound, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Materials Chemistry, [CHIM]Chemical Sciences, Electrical and Electronic Engineering, Instrumentation, Molybdenum disulfide, Graphene, 010401 analytical chemistry, Metals and Alloys, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Electrochemical gas sensor, chemistry, Differential pulse voltammetry, 0210 nano-technology, Biosensor
Abstract

International audience; Next to graphene nanomaterials, molybdenum disulfide (MoS 2) offers large surface area that can enhance its biosensing performance. In this work, we investigate the performance of glassy carbon (GC) electrodes modified successively with porous reduced graphene oxide (prGO) and molybdenum sulfide (MoS 2) for the sensitive and selective detection of the L1-major capsid protein of human papilloma virus (HPV). Owing to the difficulties to perform serological assays and HPV cultures efficiently, tools based on molecular recognition are becoming of great importance. We developed here an electrochemical sensor for HPV upon covalent functionalization of the electrode with an aptamer Sc5-c3, a RNA aptamer targeted against the HPV-16 L1 protein. Using differential pulse voltammetry (DPV) and an optimized To whom correspondence should be send to: sabine.szunerits@univ-lille.fr

Published
2018

12. Reduced graphene oxide/polyethylenimine based immunosensor for the selective and sensitive electrochemical detection of uropathogenic Escherichia coli

Author

Karima Kahlouche, Roxana Jijie, Rabah Boukherroub, Sabine Szunerits, Julie Bouckaert, Alexandre Barras, Tijani Gharbi, Nao Yamakawa, Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) [NIT / NANOMEDECINE], Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Franche-Comté (UFC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Technologie de Belfort-Montbeliard (UTBM), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)

Subjects
Electrophoretic deposition, Reduced graphene oxide, Polyethyleneimine, Anti- fimbrial E. coli antibodies, E. coli UTI89 bacteria, Electrochemical sensing, 02 engineering and technology, medicine.disease_cause, 01 natural sciences, law.invention, chemistry.chemical_compound, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Materials Chemistry, medicine, Electrical and Electronic Engineering, Instrumentation, Escherichia coli, ComputingMilieux_MISCELLANEOUS, Detection limit, Polyethylenimine, Aqueous solution, Chemistry, Potassium ferrocyanide, Graphene, 010401 analytical chemistry, Metals and Alloys, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Combinatorial chemistry, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Covalent bond, Surface modification, 0210 nano-technology
Abstract

Fast, reliable and selective detection of microorganisms is of uttermost importance in clinical analysis, but also in food and water quality monitoring. In this study, we report on the construction of an immunosensor for sensitive and selective electrochemical detection of uropathogenic Escherichia coli (E. coli) UTI89 bacteria in aqueous and serum samples. We took benefit of electrophoretic deposition (EPD) to prepare, in a simple, controllable and cost effective way, gold electrodes modified with thin active layers of reduced graphene oxide/polyethylenimine (rGO/PEI). While rGO exhibits high surface area and favourable electrochemical properties, the presence of abundant single bondNH2 groups on PEI offers a plethora of opportunities for the sensor’s surface functionalization. To achieve selectivity of detection, the electrode surface was covalently modified with anti-fimbrial E. coli antibodies via amide bond formation. To minimize non-specific adsorption, the immunosensor was additionally modified with pyrene-polyethyleneglycol (pyrene-PEG) moieties prior to antibody immobilization. The detection of E. coli was based on the restriction of electron transfer of a redox mediator, in our case potassium ferrocyanide, to the rGO/PEI modified electrical transducer due to the formation of an immune complex. The developed immunosensor displayed a sigmoidal shape with a linear range of 1 × 101–1 × 104 cfu mL−1 (R2 = 0.995) according to i(μA) = −16.66–20.5 × log[E. coli] (cfu mL−1) and a detection limit of 10 cfu mL−1. Additionally, the sensor performed well both in aqueous, serum and urine media, which is essential for its potential use for clinical diagnosis of pathogenic diseases. Selectivity studies showed that the immunosensor was able to discriminate between E. coli UTI89 wild-type strain and UTI89 Δfim, without fim operon. 260

Published
2018

13. Aqueous medium-induced micropore formation in plasma polymerized polystyrene: an effective route to inhibit bacteria adhesion

Author

Valentin Pohoata, N. Dumitrascu, Rabah Boukherroub, Ionut Topala, Alexandre Barras, Roxana Jijie, Sabine Szunerits, Julie Bouckaert, Teodora Teslaru, Tetiana Dumych, Marius Dobromir, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Danylo Halytskiy Lviv National Medical University, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
education, Biomedical Engineering, Atmospheric-pressure plasma, 02 engineering and technology, 01 natural sciences, Styrene, Contact angle, chemistry.chemical_compound, X-ray photoelectron spectroscopy, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, 0103 physical sciences, General Materials Science, Fourier transform infrared spectroscopy, ComputingMilieux_MISCELLANEOUS, 010302 applied physics, chemistry.chemical_classification, General Chemistry, General Medicine, Adhesion, Polymer, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, chemistry, Chemical engineering, Polystyrene, 0210 nano-technology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

Plasma polymerized styrene (pPS) films were successfully synthesized by means of an atmospheric pressure plasma technique, using a mixture of argon gas and styrene vapor. The morphology and film thickness of the pPS films, deposited on 1 min argon plasma pre-treated glass substrates, were smooth and uniform without any visible features across the whole length of the substrates, and the films displayed a water contact angle of ∼83°. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) analysis confirmed the presence of oxygen-containing groups and the intact aromatic ring in the pPS coating. The obtained pPS films were stable for at least 30 days in air without any visible morphological degradation or chemical changes. However, the formation of a topographical pattern with micrometer lateral size and nanometer depth level was observed upon immersion in aqueous media for 72 hours. Micropore formation was believed to originate from the solubility of low cross-linked oligomers and their subsequent extraction in aqueous media. The influence of the microstructured pPS surface in mediating the attachment of eukaryotic and prokaryotic cells was further investigated. The micro-structured pPS surface influenced the adhesion and proliferation of mammalian cells. Furthermore, we could demonstrate that these films were efficient in the prevention of Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus epidermidis (S. epidermis) adhesion and biofilm formation. Importantly, the viability of non-adherent cells and of planktonic bacteria was not affected. Post-coating of the microstructured pPS with biocompatible polydopamine did not impact on the antibacterial properties of the surface, suggesting that the polymer topography was the dominant factor. The non-biocidal pPS coating can be useful in applications where micro-organism colonization and biofilm formation need to be prevented, such as food packaging and medical equipment.

Published
2018

14. Controlled modification of electrochemical microsystems with polyethylenimine/reduced graphene oxide using electrophoretic deposition: Sensing of dopamine levels in meat samples

Author

Rabah Boukherroub, Reda Yahiaoui, Alexandre Barras, Karima Kahlouche, Ioana Silvia Hosu, Guillaume Herlem, Tijani Gharbi, Marhoun Ferhat, Roxana Jijie, Sabine Szunerits, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Nanomédecine, imagerie, thérapeutique - UFC (EA 4662) (NIT / NANOMEDECINE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Amar Telidji - Laghouat, Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) (NIT / NANOMEDECINE)

Subjects
Electrophoresis, Working electrode, Meat, Dopamine, Nanotechnology, 02 engineering and technology, Polyethylenimine, 01 natural sciences, Reference electrode, Analytical Chemistry, law.invention, [SPI.MAT]Engineering Sciences [physics]/Materials, chemistry.chemical_compound, Electrophoretic deposition, Electrochemical microsystem, law, Limit of Detection, Microsystem, Electrochemistry, Polyethyleneimine, Reduced graphene oxide, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Electrodes, [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], Graphene, 010401 analytical chemistry, Oxides, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Microelectrode, chemistry, Sensing, Surface modification, Graphite, 0210 nano-technology, Oxidation-Reduction
Abstract

International audience; Microsystems play an important role in many biological and environmental applications. The integration of electrical interfaces into such miniaturized systems provides new opportunities for electrochemical sensing where high sensitivity and selectivity towards the analyte are requested. This can be only achieved upon controlled functionalization of the working electrode, a challenge for compact microsystems. In this work, we demonstrate the benefit of electrophoretic deposition (EPD) of reduced graphene oxide/polyethylenimine (rGO/ PEI) for the selective modification of a gold (Au) microelectrode in a microsystem comprising a Pt counter and a Ag/AgCl reference electrode. The functionalized microsystem was successfully applied for the sensing of dopamine with a detection limit of 50 nM. Additionally, the microsystem exhibited good performance for the detection of dopamine levels in meat samples.

Published
2017

15. On demand electrochemical release of drugs from porous reduced graphene oxide modified flexible electrodes

Author

Samia Boulahneche, Roxana Jijie, Sabine Szunerits, Sreekumar Kurungot, Julie Bouckaert, Rabah Boukherroub, Santosh K. Singh, Fereshteh Chekin, Alexandre Barras, Mohamed Salah Medjram, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Islamic Azad University, Amol, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université 20 Août 1955 Skikda, Financial support from the Centre National de la Recherche Scientifique (CNRS), the University Lille 1, the Hauts-de-France region, the CPER 'Photonics for Society', the Agence National de la Recherche (ANR) and the EU union through FLAG-ERA JTC 2015-Graphtivity, and the Marie Sklodowska-Curie action (H2020-MSCA-RISE-2015, PANG-690836) are acknowledged. Samia Boulahneche thanks the Algerian Government for a PhD scholarship., European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Faculté des Sciences [Skikda], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Islamic Azad University, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Faculté de Technologie [Skikda], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)

Subjects
Drug, Materials science, media_common.quotation_subject, Biomedical Engineering, Oxide, Nanotechnology, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, Electrochemistry, 01 natural sciences, law.invention, chemistry.chemical_compound, law, Ampicillin, medicine, General Materials Science, media_common, Ondansetron hydrochloride, Graphene, General Chemistry, General Medicine, 021001 nanoscience & nanotechnology, Antimicrobial, Combinatorial chemistry, 0104 chemical sciences, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, chemistry, Electrode, 0210 nano-technology, medicine.drug
Abstract

International audience; Despite the advantages of an electrochemical control for drug release, only a handful of electrochemical-based release systems have been developed so far. We report herein on the development of an electrochemically activatable platform for on-demand delivery of drugs. It is based on flexible gold thin film electrodes coated with porous reduced graphene oxide (prGO) nanosheets onto which the drug of interest has been integrated beforehand. Two different drugs are investigated here: ondansetron hydrochloride (ODS), a selective 5-HT3 receptor antagonist used for preventing nausea and vomiting caused by chemotherapy and radiotherapy, and ampicillin (AMP), an antibiotic to prevent and treat a number of bacterial infections such as respiratory tract infections, urinary tract infections, and meningitis. In the case of ODS, application of a negative potential bias of −0.8 V results in a sustained slow ODS release with an ODS flux of 47 μg cm−2 h−1. In the case of AMP, we show that polyethyleneimine modified prGO (prGO/PEI) is an extremely efficient matrix. Upon the application of +0.8 V, 24% of AMP could be released from the electrical interface in a time span of 2 h. The released AMP kept its antibacterial activity as demonstrated by antimicrobial tests. These examples illustrate the major benefits of the developed approach for biomedical applications.

Published
2017

16. Selective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters

Author

Santosh K. Singh, Roxana Jijie, Fatima Halouane, Sabine Szunerits, Sreekumar Kurungot, Jean Jurazek, Rabah Boukherroub, Dalila Meziane, Julie Bouckaert, Chengnan Li, Alexandre Barras, Musen Li, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Université Mouloud Mammeri [Tizi Ouzou] (UMMTO), CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Groupe de physique des matériaux (GPM), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Shandong University, European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Université Mouloud Mammeri [Tizi Ouzou] [UMMTO], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], and Groupe de physique des matériaux [GPM]

Subjects
Materials science, Biomedical Engineering, 02 engineering and technology, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Microbiology, chemistry.chemical_compound, medicine, General Materials Science, Pathogen, Body fluid, biology, Pathogenic bacteria, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, chemistry, Surface modification, Magnetic nanoparticles, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Nanocarriers, 0210 nano-technology, Ethylene glycol, Bacteria
Abstract

International audience; The fast and efficient elimination of pathogenic bacteria from water, food or biological samples such as blood remains a challenging task. Magnetic isolation of bacteria from complex media holds particular promise for water disinfection and other biotechnological applications employing bacteria. When it comes to infectious diseases such as urinary tract infections, the selective removal of the pathogenic species in complex media such as human serum is also of importance. This issue can only be accomplished by adding pathogen specific targeting sites onto the magnetic nanostructures. In this work, we investigate the potential of 2-nitrodopamine modified magnetic particles anchored on reduced graphene oxide (rGO) nanocomposites for rapid capture and efficient elimination of E. coli associated with urinary tract infections (UTIs) from water and serum samples. An optimized magnetic nanocarrier achieves a 99.9% capture efficiency even at E. coli concentrations of 1 × 10 1 cfu mL −1 in 30 min. In addition, functionalization of the nanostructures with poly(ethylene glycol) modified pyrene units and anti-fimbrial E. coli antibodies allowed specific elimination of E. coli UTI89 from serum samples. Irradiation of the E. coli loaded nanocomposite with a near-infrared laser results in the total ablation of the captured pathogens. This method can be flexibly modified for any other pathogenic bacteria, depending on the antibodies used, and might be an interesting alternative material for a magnetic-based body fluid purification approach.

Published
2017

17. A 980nm driven photothermal ablation of virulent and antibiotic resistant Gram-positive and Gram-negative bacteria strains using Prussian blue nanoparticles

Author

Houcem Maaoui, N. Dumitrascu, Radouane Chtourou, Djamel Drider, Rabah Boukherroub, Delphine L. Caly, Julie Bouckaert, Roxana Jijie, Sabine Szunerits, Guohui Pan, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Alexandru Ioan Cuza University of Iași [Romania], Changchun Institute of Technology, Institut Charles Viollette (ICV) - EA 7394 (ICV), Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre de Biotechnologie de Borj Cédria (Hammam-Lif, Tunisie), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Gram-negative bacteria, Surface Properties, Photothermal, Near infrared, Drug Resistance, Gram negative, Virulence, Nanotechnology, 02 engineering and technology, macromolecular substances, Microbial Sensitivity Tests, 010402 general chemistry, medicine.disease_cause, Gram-Positive Bacteria, 01 natural sciences, Microbiology, Biomaterials, Colloid and Surface Chemistry, Antibiotic resistance, Microbial, Gram-Negative Bacteria, medicine, Particle Size, Escherichia coli, biology, Bacteria, Chemistry, Prussian blue, technology, industry, and agriculture, Temperature, Gram positive, Drug Resistance, Microbial, Photothermal therapy, 021001 nanoscience & nanotechnology, biology.organism_classification, Antimicrobial, Photochemical Processes, Methicillin-resistant Staphylococcus aureus, 3. Good health, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Anti-Bacterial Agents, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Nanoparticles, 0210 nano-technology, Ferrocyanides
Abstract

A 980nm laser-driven antimicrobial photothermal therapy using poly(vinylpyrrolidone) -coated Prussian Blue nanoparticles (PVP/PB NPs) is demonstrated. This approach allows an efficient eradication of a virulent strain of Gram-negative Escherichia coli (E. coli) associated with urinary tract infection as well as for the ablation of antibiotic resistant pathogens such as methicillin resistant Staphylococcus aureus (MRSA) and extended spectrum β-lactamase (ESBL) E. coli. Interestingly the 980nm irradiation exhibits minimal effect on mammalian cells up to a PVP/PB NPs concentration of 50μgmL(-1), while at this concentration bacteria are completely eradicated. This feature is certainly very promising for the selective targeting of bacteria over mammalian cells.

Published
2016

18. Nanomaterials for transdermal drug delivery: beyond the state of the art of liposomal structures

Author

Rabah Boukherroub, Roxana Jijie, Sabine Szunerits, Alexandre Barras, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), and European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016)

Subjects
Drug, skin, media_common.quotation_subject, Biomedical Engineering, Nanotechnology, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 030226 pharmacology & pharmacy, 03 medical and health sciences, 0302 clinical medicine, Stratum corneum, medicine, [CHIM]Chemical Sciences, General Materials Science, media_common, Transdermal, Liposome, Iontophoresis, business.industry, drug, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, patches, 3. Good health, medicine.anatomical_structure, [CHIM.POLY]Chemical Sciences/Polymers, Targeted drug delivery, Drug delivery, nanoparticles, transdermal delivery, 0210 nano-technology, Drug carrier, business
Abstract

International audience; A wide range of biomedical materials have been proposed to meet the different needs for controlled oral or intravenous drug delivery. The advantages of oral delivery such as self-administration of a predetermined drug dose at defined time intervals makes it the most convenient means for the delivery of small molecular drugs. It fails however to delivery therapeutic macromolecules due to rapid degradation in the stomach and size-limited transport across the epithelium. The primary mode of administration of macromolecules is presently via injection. This administration mode is not without limitations, as the invasive nature of injections elicits pain and decreases patients' compliance. Alternative routes for drug delivery have been looked for, one being the skin. Delivery of drugs via the skin is based on the therapeutics penetrating the stratum corneum with the advantage of overcoming first-pass metabolism of drugs, to deliver drugs with a short-half-life time more easily and to eliminate frequent administrations to maintain constant drug delivery. The transdermal market still remains limited to a narrow range of drugs. The low permeability of the SC to water-soluble and macromolecular drugs poses significant challenges to transdermal administering via passive diffusion through the skin, as is the case for all topically administered drug formulations intended to bring the therapeutic into the general circulation. To widen the scope of drugs for transdermal delivery, new procedures to enhance skin permeation to hydrophilic drugs and macromolecules are under development. Next to the integration of skin enhancers into pharmaceutical formulations, nanoparticles based on lipid carrier have been widely considered and reviewed. While being briefly reviewed here, the main focus of this article is on current advancements using polymeric and metallic nanoparticles. Next to these passive technologies, the handful of active technologies for local and systemic transdermal drug delivery will be discussed and put into perspective. While passive approaches dominate the literature and the transdermal market, active delivery patches based on microneedles or iontophoresis approaches have shown great promise for transdermal drug delivery and have entered the market, in the last decade. This review gives an overall idea of the current activities in this field.

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