1. Effect of post-rinsing time on the mechanical strength and cytotoxicity of a 3D printed orthodontic splint material
- Author
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Yichen Xu, Alexander B. Xepapadeas, Bernd Koos, Jürgen Geis-Gerstorfer, Ping Li, and Sebastian Spintzyk
- Subjects
Materials science ,Surface Properties ,Scanning electron microscope ,medicine.medical_treatment ,02 engineering and technology ,Surface finish ,Bending ,Methacrylate ,03 medical and health sciences ,0302 clinical medicine ,Flexural strength ,Flexural Strength ,Materials Testing ,medicine ,General Materials Science ,Viability assay ,Composite material ,General Dentistry ,Aqueous solution ,030206 dentistry ,021001 nanoscience & nanotechnology ,Splints ,Mechanics of Materials ,Printing, Three-Dimensional ,0210 nano-technology ,Splint (medicine) - Abstract
Objective Since the post-rinsing time is inconsistently recommended, this study aims to investigate the effect of post-rinsing time on the flexural strength and cytotoxicity of an stereolithographically (SLA) printed orthodontic splint material. Methods SLA-printed specimens were ultrasonically rinsed with isopropanol (IPA) for 5 min, 12 min, 20 min, 30 min, 1 h, and 12 h, respectively. Surface characterization was conducted by scanning electron microscopy and roughness measurements. Flexural strength was evaluated using a three-point bending test. Cytotoxicity was determined by direct contact test and extract test. For both tests, cell viability (live/dead staining) and cell metabolic activity (CCK-8 assay) were evaluated. Additionally, water sorption and water solubility were tested to analyze the mass loss from immersion. Results No apparent surface alterations could be detected on the samples post-rinsed for less than 1 h. In contrast, when the post-rinsing time was prolonged to 12 h, surface fissures could be observed. Flexural strength linearly decreased with increasing post-rinsing time. All post-processed specimens did not show an obvious cytotoxic effect. Significance The removal of cytotoxic methacrylate monomers by post-rinsing with IPA could be achieved in 5 min. Extending post-rinsing time could not improve the cytocompatibility of the SLA-printed orthodontic splint material, and may result in a decrease in flexural strength.
- Published
- 2021