1. Durable endothelium-mimicking coating for surface bioengineering cardiovascular stents
- Author
-
Xiuying Shi, Xing Tan, Zhilu Yang, Kaiqin Xiong, Manfred F. Maitz, Qing Ma, Nan Huang, Yuan Yuan Cui, Qiufen Tu, Li Shen, Rui Wang, and Zhangmei Hu
- Subjects
Endothelium mimicking ,Intimal hyperplasia ,Endothelium ,QH301-705.5 ,medicine.medical_treatment ,Glycocalyx component ,0206 medical engineering ,Biomedical Engineering ,Cardiovascular stents ,02 engineering and technology ,engineering.material ,Article ,Biomaterials ,Glycocalyx ,chemistry.chemical_compound ,Restenosis ,Coating ,medicine ,Biology (General) ,Materials of engineering and construction. Mechanics of materials ,Carbodiimide ,Chemistry ,Surface bioengineering ,Stent ,Nitric oxide ,Heparin ,021001 nanoscience & nanotechnology ,medicine.disease ,020601 biomedical engineering ,medicine.anatomical_structure ,engineering ,TA401-492 ,0210 nano-technology ,Biotechnology ,Biomedical engineering ,medicine.drug - Abstract
Mimicking the nitric oxide (NO)-release and glycocalyx functions of native vascular endothelium on cardiovascular stent surfaces has been demonstrated to reduce in-stent restenosis (ISR) effectively. However, the practical performance of such an endothelium-mimicking surfaces is strictly limited by the durability of both NO release and bioactivity of the glycocalyx component. Herein, we present a mussel-inspired amine-bearing adhesive coating able to firmly tether the NO-generating species (e.g., Cu-DOTA coordination complex) and glycocalyx-like component (e.g., heparin) to create a durable endothelium-mimicking surface. The stent surface was firstly coated with polydopamine (pDA), followed by a surface chemical cross-link with polyamine (pAM) to form a durable pAMDA coating. Using a stepwise grafting strategy, Cu-DOTA and heparin were covalently grafted on the pAMDA-coated stent based on carbodiimide chemistry. Owing to both the high chemical stability of the pAMDA coating and covalent immobilization manner of the molecules, this proposed strategy could provide 62.4% bioactivity retention ratio of heparin, meanwhile persistently generate NO at physiological level from 5.9 ± 0.3 to 4.8 ± 0.4 × 10−10 mol cm−2 min−1 in 1 month. As a result, the functionalized vascular stent showed long-term endothelium-mimicking physiological effects on inhibition of thrombosis, inflammation, and intimal hyperplasia, enhanced re-endothelialization, and hence efficiently reduced ISR., Graphical abstract Image 1, Highlights • A durable endothelium-mimicking coating was developed for surface bioengineering of cardiovascular stents. • The durable endothelium-mimicking surface was realized by stepwise grafting of Cu-DOTA and heparin on a robust coating. • The durable endothelium-mimicking coating endows the vascular stents with ability to dramatically reduce restenosis.
- Published
- 2021