1. LISzyme Biosensors: DNAzymes Embedded in an Anti-biofouling Platform for Hands-free Real-Time Detection of Bacterial Contamination in Milk
- Author
-
Hanie Yousefi, Sahar Esmaeili Samani, Shadman Khan, Akansha Prasad, Amid Shakeri, Yingfu Li, Carlos D. M. Filipe, and Tohid F. Didar
- Subjects
Bacteria ,General Engineering ,General Physics and Astronomy ,Food Contamination ,Biosensing Techniques ,DNA, Catalytic ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Milk ,Escherichia coli ,Animals ,General Materials Science ,0210 nano-technology ,Lubricants - Abstract
Nonspecific binding is a significant challenge associated with biosensors in complex food textures. To overcome this, we have developed LISzymes, which are DNAzymes incorporated in lubricant-infused surfaces (LISs). Using milk as a complex background matrix, we show that LISzyme biosensors are significantly more effective in preventing nonspecific binding compared to other commonly used "blocking" methods. The use of lubricant infusion to treat sensing surfaces results in a 4-fold increase in the signal-to-noise ratio obtained with the DNAzyme with respect to untreated surfaces, when detecting the presence of specific bacteria in milk. This is a striking improvement upon previous DNAzyme sensors. We also show that the use of LISs does not affect the DNAzyme's ability to effectively and specifically detect its target─a protein specifically produced by
- Published
- 2021