1. Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells
- Author
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Hongyu Shen, James D. Higgins, Ruifeng Fu, Chong Wang, Jie Zhang, and Wanqi Liang
- Subjects
0106 biological sciences ,0301 basic medicine ,DNA repair ,RAD51 ,Synapsis ,homologous recombination ,Plant Science ,lcsh:Plant culture ,Biology ,BRCA2 ,01 natural sciences ,Double Strand Break Repair ,Cell biology ,03 medical and health sciences ,DMC1 ,030104 developmental biology ,Meiosis ,Homologous chromosome ,meiosis ,lcsh:SB1-1110 ,Homologous recombination ,Original Research ,010606 plant biology & botany - Abstract
The mammalian BREAST CANCER 2 (BRCA2) gene is a tumor suppressor that plays a crucial role in DNA repair and homologous recombination (HR). Here, we report the identification and characterization of OsBRCA2, the rice orthologue of human BRCA2. Osbrca2 mutant plants exhibit normal vegetative growth but experience complete male and female sterility as a consequence of severe meiotic defects. Pairing, synapsis and recombination are impaired in osbrca2 male meiocytes, leading to chromosome entanglements and fragmentation. In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1. These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination. In addition, treatment of osbrca2-1 seedlings with mitomycin C (MMC) led to hypersensitivity. As MMC is a genotoxic agent that creates DNA lesions in the somatic cells that can only be repaired by HR, these results suggest that OsBRCA2 has a conserved role in DSB repair and HR in rice.
- Published
- 2020