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Despite all the applications of nanotechnology, limited data are available on their environmental and health risks. Many nanomaterials have been developed; however, their safety evaluation is not performed at the same speed. Toxicity can occur at different trophic levels (microorganisms, invertebrates, and vertebrates), and because of that, alternative/complementary models have been successfully employed. This mini-review aims to report some studies that have successfully used different alternative models to assess nanotoxicology and to stimulate their use by other groups of this important field. This will provide more information about nanomaterials and also to evoke studies to improve formulations.