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A pilot-scale multistage constructed wetland-pond (MCWP) system with a "pre-ecological oxidation pond, two-stage horizontal subsurface flow constructed wetland (HSCW) and surface flow constructed wetland (SFCW) as the core and postsubmerged plant pond" as the process was used to treat actual polluted river water in the field, and the variation in nitrogen removal from summer to winter was investigated. The results showed that the average total nitrogen (TN) removal efficiency in the MCWP was approximately 40.74%. The significant positive correlation between the daily highest temperature and the TN removal efficiency of the whole system was fitted with a nonlinear curve (R2 = 0.7192). The TN removal load rate in the HSCWs was 2.7–3.7 times that in the SFCW. The SFCW, which had high-density plants (35 plants/m2), increased the proportion of nitrogen removed by plant harvesting and microbial function. The TN transformed by Iris pseudacorus L. accounted for 54.53% in the SFCW. Furthermore, bacteria completed the nitrogen cycle in the SFCW through a variety of nitrogen removal pathways. This research not only investigated the TN removal performance in an MCWP system but also made it possible to predict the TN removal efficiency according to the daily highest temperature from summer to winter in the field.

The pharmaceutical and personal care product (PPCP) residues in freshwater lakes are being highlighted around the world. The occurrence and ecological risk of 34 PPCPs classified as antibiotics, non-steroidal anti-inflammatory drugs (NSAID), cardiovascular drugs, psychotropic drugs, anti-inflammatory drugs, psychostimulants, and pesticides during rainstorm period in surface water of the Dongting Lake, China, were studied. Twenty-six out of thirty-four PPCPs were detected, and the total concentrations of antibiotics ranged from 0.15 to 214.75 ng L−1 in surface water. The highest average concentration was observed for diclofenac, followed by diethyltoluamide (DEET). The PPCP concentrations were much lower in Dongting Lake compared to other rivers and lakes due to the strong dilution effect of rainstorm, while the detection rate remains high. Caffeine and DEET were detected with 100% frequency in Dongting Lake, and the detection rates of diclofenac, mefenamic acid, and roxithromycin were above 90%. The pollution levels of antibiotics decreased in the order of East Dongting Lake > South Dongting Lake > West Dongting Lake, which may be related to the distribution of aquaculture plants, sewage treatment plants, and population density. The risk quotient (RQ) method was used to evaluate ecological environment risk under the worst case and the results suggested that clarithromycin, diclofenac, roxithromycin, and erythromycin might pose a significant risk to aquatic organisms in Dongting Lake, especially clarithromycin. This study can provide data support for further research on the dilutive effect and mechanism of rainwater runoff on PPCPs in lakes on a large scale.

Plant-microbe is a complementary coupling system for antibiotics removing in constructed wetlands (CWs), but how plant and rhizosphere microbiomes respond to antibiotics exposure and the occurrence of ARGs in this microenvironment have seldom been researched. Thus, the response of the plant-microbe coupling system to different levels of antibiotics (sulfamethoxazole (SMZ) and ofloxacin (OFL)) was investigated. The results showed that two antibiotic stressors have hormetic effects on plant growth, physiology, and microbial community evolution, and the antibiotic toxic effects presented as SMZ + OFL > SMZ > OFL. Antibiotic accumulation in the plants was in the order of roots > stems > leaves. Notably, the root attachments affected antibiotic transportation. The accumulation of antibiotics in the under-ground parts affected the rhizosphere microbial community structure, and the microorganisms were more sensitive to SMZ + OFL than the plants, with inflection points of 0.5 mg L−1 and 1 mg L−1, respectively. Pseudomonas was highly resistant to antibiotics, while Acidovorax and Devosia may play a role in antibiotic degradation. Correlation analysis and network analysis showed that antibiotic enrichment and the bacterial community contributed significantly to the abundance of antibiotic-resistant genes (ARGs), further revealing the co-occurrence of int1, ARGs, and the potential bacterial hosts.

To investigate the effect of a constructed wetland (CW) with steel slag as the filler on water contaminated by low phosphorus levels, a multistage pond CW system was designed in this study. Low-phosphorus polluted river water was used as the research object. This study explored the effects of using steel slag as a CW filler on phosphorus removal and the total phosphorus (TP) purification effect of the wetland system. The results showed that the TP removal rates in the ecological pond, oxidation pond, surface flow wetlands and submerged plant pond were 5.17%, 8.02%, 21.56%, and 16.31%, respectively. Intermittent increases in phosphorus concentration were observed in the reactors and were caused by the decay of plant tissues, which released pollutants. Because steel slag was added to the filler, the TP concentrations in the effluent of the first- and second-level horizontal subsurface CWs increased by 151.13% and 16.29%, respectively, compared to the influent concentration. The 20th to 40th days of the test run was a period of rapid phosphorus release of the system. The use of steel slag has a potential risk of phosphorus release when applied in CWs used to purify low-phosphorus contaminated water bodies.

The residues of organophosphorus pesticides (OPPs) have been widely detected in rivers, the gulf, and even groundwater and drinking water, which may pose a serious threat to aquatic ecosystems and human health. Compared to other treatments, constructed wetlands (CWs) have been demonstrated to be a cost-effective alternative risk mitigation strategy for non-point-source pesticide pollution. This review summarizes 32 studies related to the remediation of OPPs in 117 CWs during 2001–2017 worldwide. The performances, mechanisms and influencing factors in the studies are comprehensively and critically reviewed in this paper. Overall, the OPPs were efficiently removed with an efficiency up to 87.22 ± 16.61%. The removal efficiency, differences and related reasons among different types of CWs in developed and developing countries and the different types of OPPs in CWs are well-evaluated in detail. In addition, the main processes for OPPs removal in CWs involve phytoremediation (plant uptake, phytoaccumulation, phytovolatilization and phytodegradation), substrate adsorption or sedimentation, and biodegradation. Based on the quantitative analysis by mass balance, for water-soluble pesticides, the dominant removal process was via microbiological degradation. This result was in contrast to findings obtained with hydrophobic OPPs, for which the dominant processes were biodegradation and sorption by substrate. Therefore, the behavior of microbial transformation prevails. Additionally, the presence of plants can facilitate the elimination of OPPs in CWs, promoting the process by an average percentage of approximately 6.19 ± 9.46%. Statistical analysis shows that loading of inlet OPPs is the largest limiting factor and that the HRT and T are the most significant parameters that influence the efficiency of trapping OPPs in CWs. Simultaneously, we can also obtain suitable parameters for the design and operation of CWs. This review promotes further research on plant-microbe joint combined remediation and examines the different behaviors of water-soluble and hydrophobic OPPs in CWs.

The efficiency of nitrogen removal and its mechanism, aquatic organism distribution characteristics and regeneration capability of zeolite from an integrated vertical-flow constructed wetland (IVFCW) for low pollution water treatment were evaluated after steady and continuous operation for eight years. After running for eight years, better than average COD and NH4+-N removal were observed in the IVFCW. The NH4+-N removal rate in this system was controlled by ammoxidation and adsorption and ion exchange of zeolite. The low total nitrogen (TN) removal efficiency was due to NO3−-N accumulation and zeolite desorption. In addition, this phenomenon indicated that because of poor organic carbon sources, nitrification was stronger than denitrification, consistent with the distribution of the functional genes for nitrification and denitrification. The biological activity in this system was abundant, especially that of spirogyra and navicula. The saturated adsorption capacity of zeolite was as high as 1.35 mg g−1 with a desorption rate of 50%). However, the drained reoxygenation performance of was better due to zero energy consumption and regeneration in situ.

The antibiotics residues in freshwater lakes are being highlighted around the world because of high potential threat to environment and human health. Understanding the current state of antibiotics and potential sources in lakes are important. The potential sources of antibiotics (Sewage treatment plants (STPs)), livestock and poultry farms and fishponds in the East Dongting was studied. Compared with other surface water bodies, the concentration of antibiotic in the East Dongting Lake was at a moderate or low level. Ten of 12 antibiotics likely originated from veterinary applications in livestock and poultry farms, especially in swine farms, and concentrations at these sources (ND-1240.41 ng L−1) were 1–3 orders of magnitude higher than in the effluent of local sewage treatment plants and fishponds. Based on a principal component analysis with multiple linear regression (PCA-MLR) model, we estimated source contributions of 79.95% for livestock and poultry farms, 0.27% for STPs, and 19.79% for aquaculture source and livestock and poultry farms. Overall, the predominance of sulfonamides and livestock and poultry farms in the East Dongting Lake has been identified, which can provide important information for regulating their veterinary use and environmental management.

Perfluorododecanoic acid (PFDoA), a kind of perfluorinated carboxylic acid (PFCA) with 12 carbon atoms, has an extensive industrial utilization and is widespread in both wildlife and the water environment, and was reported to have the potential to cause a disruption in the thyroid hormone system homeostasis. In this study, zebrafish embryos/larvae were exposed to different concentrations of PFDoA (0, 0.24, 1.2, 6 mg/L) for 96 h post-fertilization (hpf). PFDoA exposure caused obvious growth restriction connected with the reduced thyroid hormones (THs) contents in zebrafish larvae, strengthening the interference effect on the growth of fish larvae. The transcriptional level of genes within the hypothalamic-pituitary-thyroid (HPT) axis was analyzed. The gene expression levels of thyrotropin-releasing hormone (trh) and corticotrophin-releasing hormone (crh) were upregulated upon exposure to 6 mg/L of PFDoA, and iodothyronine deiodinases (dio2) was upregulated in the 1.2 mg/L PFDoA group. The transcription of thyroglobulin (tg) and thyroid receptor (trβ) were significantly downregulated upon exposure to 1.2 mg/L and 6 mg/L of PFDoA. PFDoA could also decrease the levels of sodium/iodide symporter (nis) and transthyretin (ttr) gene expression in a concentration-dependent manner after exposure. A significant decrease in thyroid-stimulating hormoneβ (tshβ), uridinediphosphate-glucuronosyltransferase (ugt1ab) and thyroid receptor (trα) gene expression were observed at 6 mg/L PFDoA exposure. Upregulation and downregulation of iodothyronine deiodinases (dio1) gene expression were observed upon the treatment of 1.2 mg/L and 6 mg/L PFDoA, respectively. All the data demonstrated that gene expression in the HPT axis altered after different PFDoA treatment and the potential mechanisms of the disruption of thyroid status could occur at several steps in the process of synthesis, regulation, and action of thyroid hormones.

Microcystin-LR (MCLR) has been reported to cause developmental neurotoxicity in zebrafish, but there are few studies on the mechanisms of MCLR-induced transgenerational effects of developmental neurotoxicity. In this study, zebrafish were exposed to 0, 1, 5, and 25 μg/L MCLR for 60 days. The F1 zebrafish embryos from the above-mentioned parents were collected and incubated in clean water for 120 h for hatching. After examining the parental zebrafish and F1 embryos, MCLR was detected in the gonad of adults and F1 embryos, indicating MCLR could potentially be transferred from parents to offspring. The larvae also showed a serious hypoactivity. The contents of dopamine, dihydroxyphenylacetic acid (DOPAC), serotonin, gamma-aminobutyric acid (GABA) and acetylcholine (ACh) were further detected, but only the first three neurotransmitters showed significant reduction in the 5 and 25 μg/L MCLR parental exposure groups. In addition, the acetylcholinesterase (AChE) activity was remarkably decreased in MCLR parental exposure groups, while the expression levels of manf, bdnf, ache, htr1ab, htr1b, htr2a, htr1aa, htr5a, DAT, TH1 and TH2 genes coincided with the decreased content of neurotransmitters (dopamine, DOPAC and serotonin) and the activity of AChE. Neuronal development related genes, α1-tubulin, syn2a, mbp, gfap, elavl3, shha and gap43 were also measured, but gap43 was the gene only up-regulated. Our results demonstrated MCLR could be transferred to offspring, and subsequently induce developmental neurotoxicity in F1 zebrafish larvae by disturbing the neurotransmitter systems and neuronal development.

Poyang Lake is the largest freshwater lake in China and plays a significant role by supplying drinking water, enabling agriculture, and preventing floods. Random selection was implemented to obtain 36 samples of surface deposits to determine concentrations of Pb, Cd, Cu, Zn, Cr, and Ni across Poyang Lake. At all sampling locations, values of Ni and Cd were thought to be lower than the effect range low (ERL). Relatively, values of Cu, Pb, Cr, and Zn were classified as ERL-effect range median (ERM) in more than 30% of sampling locations. In general, the eastern areas of Poyang Lake near the inflow rivers, such as the Raohe, Xinjiang, and Fuhe rivers, had higher concentrations of heavy metals. These rivers are sources of substantial industrial wastewater, including a lot of heavy metals. In addition, higher toxicity of sediments was found in the eastern area of the lake by evaluating the sum of toxic units (ΣTU). Contributions of Pb and Cr to the sum of toxic units were higher than the other heavy metals. If the heavy metals were classified by contribution to toxic units in Poyang Lake in decreasing order, they would be listed as Cr, Ni, Cd, Zn, Cu, and Pb.

The potential risk to human health of antibiotics that pass through the food chain has become an important global issue, but there are few reports on the response of ginger (Zingiber officinale) to antibiotic pollution. In this study, we investigated the enrichment characteristics and biological response of ginger to sulfamethoxazole (SMZ) and ofloxacin (OFL) residues, which are common in the environment. Lower levels of SMZ, OFL and their combined duplex treatment (SMZ+OFL) promoted the growth of ginger, but the critical doses necessary to stimulate growth differed among treatments: 10 mg L−1 SMZ, 1 mg L−1 OFL and 1 mg L−1 (SMZ+OFL) had the strongest stimulating effects. At higher dosages, the root growth and light energy utilization efficiency of ginger were impaired, and (SMZ+OFL) had the strongest inhibitory effect. Treatments with lower levels of antibiotics had no significant effect on reactive oxygen species and antioxidant enzyme activities. However, when SMZ, OFL and SMZ+OFL concentrations exceeded 10 mg L−1, the contents of H2O2, O2− and MDA continued to increase, while the activities of SOD, POD, CAT first increased and then decreased, especially in SMZ+OFL. Ginger accumulated more SMZ and OFL in rhizomes and less in leaves, and accumulation increased significantly as antibiotic concentration increased. When SMZ concentration was 1 mg L−1, the SMZ concentrations in rhizomes, roots, and leaves were 0.23, 0.15, and 0.05 mg kg−1, respectively, and the residual SMZ in the rhizome was 2.3 times higher than the maximum residue limit. The abundance of the resistance genes sul1, sul2, qnrS, and intI1 increased with increasing antibiotic concentrations, and intI1 abundance was the highest. OFL induced higher levels of intI1 expression than did SMZ.

Antibiotics are widely detected in the water environment, posing a serious threat to the health of humans and animals. The effect of levofloxacin (LOFL) on pollutant removal and the difference in the influence mechanisms at normal and low temperatures in constructed wetlands are worth discussing. A hydroponic culture experiment was designed with Iris pseudacorus L. at low and normal temperatures. LOFL (0–100 µg/L) was added to the systems. The results indicated that the removal of pollutants was affected most by temperature, followed by LOFL concentration. At the same concentration of LOFL, the pollutant removal rate was significantly higher at normal temperature than at low temperature. Low concentrations of LOFL promoted the degradation of pollutants except TN under normal-temperature conditions. Compared with the results at low temperature, the bacterial community richness was higher and the diversity of bacterial communities was lower under normal-temperature conditions. The genera and the function of bacteria were greatly affected by antibiotic concentration, temperature and test time. A series of microorganisms resistant to antibiotics and low temperature were identified in this study. The results will provide valuable information and a reference for our understanding of the ecological effects of LOFL.

Polycyclic aromatic hydrocarbons (PAHs) residues have attracted attention worldwide. This study summarizes the current levels of PAH exposure in the water environments of lakes. In addition, the risk levels from individual PAHs and ΣPAHs in the water environments of lakes in China were evaluated by incremental lifetime cancer risk (ILCR) assessment, the toxic equivalent concentration (TEQBaP), the risk quotient (RQ), the effects range-low (ERL) and the effects range-median (ERM). The results showed that the concentrations of ∑PAHs in water and sediment ranged from 4.0 to 12,970.8 ng L−1 and 6.52 to 7935.21 ng g−1, respectively, and the highest concentrations of individual PAHs were of naphthalene (Nap) (6525 ng L−1), followed by indeno(1,2,3‑cd)pyrene (IcdP) (3452.6 ng g−1). Concentrations in the Great Lakes region in China showed spatial difference, with the Qinghai-Tibet Plateau Lakes District and Mongolia-Xinjiang Lakes District being less polluted. However, the pollution level of PAHs in lakes is relatively high at the global scale. The ecological risk assessment found a moderate level of ∑PAHs in water, but benz(a)anthracene (BaA) and phenanthrene (Phe) had high RQ values, which might pose a significant risk to aquatic organisms in lakes. Although the contents of ∑PAHs in sediments are low, most individual PAHs pose potential risks, especially acenaphthene (Ace), fluorene (Flu) and dibenz(a,h)anthracene (DahA). This study revealed the pollution levels of PAHs across China and provided a scientific basis for PAH pollution control and environmental protection.

The pollution of antibiotics and antibiotic resistance genes (ARGs) has been highlighted on a global scale because of their serious threats to the environment and human health. Typical urban water in cities with high population density are ideal mediums for the acquisition and spread of antibiotics and ARGs. The pollution level of a broad range of antibiotics and ARGs in hospital wastewater, groundwater and the Wenyu River, and their fates through three sewage treatment plants (STPs) were investigated in this study. The concentrations of the 11 detected antibiotics ranged from not detected (ND)-16800 ng L−1 in diverse water samples from Beijing, and fluoroquinolones were detected at the highest concentration, especially in the hospital samples. The maximum concentrations of antibiotics in STPs and hospital were 1–3 orders of magnitude higher than those in the surface water from Wenyu River and groundwater. Good removal efficiencies by treatment processes were observed for tetracyclines and quinolones, and low removal efficiencies were observed for sulfonamides and macrolides. These results also revealed that the sulfonamide resistance genes (sul1, sul2) and macrolide resistance genes (ermB) were detected at the highest relative abundances (7.11 × 10−2-1.18 × 10−1) in the water bodies of Beijing. It was worth noting that sul1 abundance was the highest in groundwater samples. The relative abundance of most ARGs in STPs exhibited a declining trend in the order of influent > secondary effluents > effluent. However, the relative abundance of sul 1, sul 2 and tetC in the effluent was higher than those in the influent. The incomplete removal of antibiotics and ARGs in STPs poses a serious threat to the receiving rivers, and affects ecosystem security. Overall, our findings provide favorable support for a further investigation of the spread and risk of antibiotics and ARGs from diverse sources (e.g., STPs and hospitals) to the aquatic environment.

In this study, 15 representative surface waters and sediments and seven typical fish were collected during the wet season in 2016 to explore the occurrence, chemical fractionation, and ecological risk of heavy metals (Cu, Zn, Pb, Cd, Cr, Hg, As, and Ni) in aquatic ecosystems of Dongting Lake in China. In surface water, the concentrations of all elements were lower than the third grade of the surface water quality standards (GB3838-2002). The highest concentrations of Zn, Pb, Cd, Ni, and Cu were found in the outlet of Dongting Lake, whereas As and Cr were highest in the Xiangjiang River Delta area. In surface sediments, the concentration decreased in the order of Zn > Cr > Pb > Cu > Ni > As>Cd > Hg; the highest concentration of all elements, except for Hg, was located in the Xiangjiang River Delta area; for chemical fractionation, Cr, Hg, Zn, As, Ni, and Cu were mainly residual fractionation; and Pb and Cd were mainly in a reducible state and fraction soluble in acid, respectively. In fish muscle, the concentrations of all elements were lower than relevant standards; the highest concentration appeared in fish living in the middle-lower and demersal layers. As for the feeding habits of fish, the average concentration decreased in the order of carnivorous > omnivorous > herbivorous fish. The potential ecological risk and the ratio of secondary phase to primary phase assessment showed that Cd and Hg had the highest ecological risk and that Cd and Pb had a high risk of secondary release. A health risk assessment showed that drinking water and fish for consumption in urban and rural areas around Dongting Lake were in the acceptable level.

Signatures of stable isotope ratios have previously been used to trace the source and transport of particulate organic matter (POM) in freshwater and marine ecosystems. In this study, water columns were collected at 22 sites in the littoral zone of Lake Taihu in 2014 to investigate the distribution and concentration of nutrients and the stable isotope signatures of POM, and their potential interrelation. Generally, mean concentration of nitrogen forms (NH4+-N) showed substantial variation, probably because they had received large amounts of wastewater from various local industrial enterprises. Source analysis by stable carbon and nitrogen isotopic ratios showed that the main POM sources were phytoplankton. Contrasting correlations were obtained between δ15N and N concentrations in effluent river mouths when compared with influent river mouths. In effluent river mouths, there was a significant positive correlation between δ15N and nitrogen concentration (total nitrogen and ammonia-nitrogen), in contrast with the negative correlation in influent river mouths. According to these results, more factors should be taken into consideration when stable carbon and nitrogen isotopes of POM are used to assess the feeding relationship between consumers and prey, as well as the energy flow pathways that support the lake pelagic food webs.

Pollution caused by antibiotics has been highlighted in recent decades as a worldwide environmental and health concern. Compared to traditional physical, chemical and biological treatments, constructed wetlands (CWs) have been suggested to be a cost-efficient and ecological technology for the remediation of various kinds of contaminated waters. In this review, 39 antibiotics removal-related studies conducted on 106 treatment systems from China, Spain, Canada, Portugal, etc. were summarized. Overall, the removal efficiency of CWs for antibiotics showed good performance (average value = over 50%), especially vertical flow constructed wetlands (VFCWs) (average value = 80.44%). The removal efficiencies of sulfonamide and macrolide antibiotics were lower than those of tetracycline and quinolone antibiotics. In addition, the relationship between the removal efficiency of antibiotics and chemical oxygen demand (COD), total suspended solids (TSS), total nitrogen (TN), total phosphorus (TP) and ammonia nitrogen (NH3-N) concentrations showed an inverted U-shaped curve with turning points of 300 mg L−1, 57.4 mg L−1, 40 mg L−1, 3.2 mg L−1 and 48 mg L−1, respectively. The coexistence of antibiotics with nitrogen and phosphorus slightly reduced the removal efficiency of nitrogen and phosphorus in CWs. The removal effect of horizontal subsurface flow constructed wetlands for antibiotic resistance genes (ARGs) had better performance (over 50%) than that of vertical wetlands, especially for sulfonamide resistance genes. Microorganisms are highly sensitive to antibiotics. In fact, microorganisms are one of the main responsible for antibiotic removal. Moreover, due to the selective pressure induced by antibiotics and drug-resistant gene transfer from resistant bacteria to other sensitive strains through their own genetic transfer elements, decreased microbial diversity and increased resistance in sewage have been consistently reported. This review promotes further research on the removal mechanism of antibiotics and ARGs in CWs.

According to current study, seven surface sediments and three sediment cores were collected from three typical areas of Tiaoxi River, which were living area, agricultural area, and industrial area. The water quantity into the Lake Taihu from the Tiaoxi River accounted for almost one third of the total water quantity by all rivers into the Lake Taihu. To study geochemical features and pollution history of heavy metals in three typical areas of Tiaoxi River, total content and chemical fractionations of Cu, Pb, Cd, Cr, Mn, Zn, Fe, As, and Hg were analyzed for surface and core sediments using the speciation extraction procedure, proposed by the Commission of the European Communities Bureau of Reference (BCR), together with grain size and organic carbon measurements. The results showed that the concentration of nine heavy metals and the variation characteristics of Zn, Cu, Fe, Mn, and Cr are different among five cores, which has shown that the river responses to natural and anthropogenic activities were dissimilar in various areas. BCR sequential extraction showed contents of Cr, Fe, and Cu were dominated in the remaining parts. Non-residual fractions for Zn and Mn contained major portions. Based on RAC (risk assessment core), the risk of Mn was high to very high in the three typical areas, and the risk of Zn was medium in the three typical areas.

Based on the data analysis of the water environmental quality and economic development from 2002 to 2012 in the Nansi Lake basin, the correlation and change between the water environmental quality and economic development were studied. Results showed that the GDP and wastewater emissions of point source in the Nansi Lake basin had an average annual growth of 7.30 and 7.68 %, respectively, from 2002 to 2012. The emissions of chemical oxygen demand (COD) and ammonia nitrogen (NH3-N) had the average annual decrease of 7.69 and 6.79 % in 2012, respectively, compared to 2002. Basin water quality overall improved, reaching the Class III of the "Environmental quality standards for surface water (GB3838-2002)," in which the main reason was that sewage treatment rate increased gradually and was above 90 % in 2012 (an increase of 10 % compared to 2002) with the progress of pollution abatement technology and the implementation of relevant policies and regulations. The contribution of water environmental pollution was analyzed from related cities (Ji'ning, Zaozhuang, Heze). Results indicated that Ji'ning had the largest contribution to water pollution of the Nansi Lake basin, and the pollutant from domestic sources accounted for a higher percentage compared to industrial sources. The wastewater, COD, and NH3-N mainly came from mining and washing of coal, manufacture of raw chemical materials and chemical products, papermaking industry, and food processing industry. According to the water pollution characteristics of the Nansi Lake basin, the basin pollution treatment strategy and prevention and treatment system were dissected to provide a scientific basis for prevention and control of lakeside point source pollution along the Nansi Lake.