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Background Abelmoschus ficulneus (L.) Wight & Arn. (Malvaceae) commonly known as herb in the Ayurveda, Indian traditional medicine system. Herb is reported as a Rasayana that has the potential to produce immunomodulatory effects. In the present study, the immunomodulatory effect of aqueous (AEAF) and ethanolic (EEAF) leaves extract of A. ficulneus (L.) Wight & Arn. in different experimental animal models were evaluated. Results Acute oral toxicity shows that both extracts have wide range of safety as per OECD 420 guidelines. Quantitative and qualitative analysis was evaluated by performing preliminary phytochemical screening, thin layer chromatography and high-performance thin layer chromatography developed for simultaneous quantification of flavonoids (quercetin) in both the extracts. Oral administration of A. ficulneus L. extracts showed a significant increase in the primary and secondary humoral antibody responses against cyclophosamide (100 mg/kg) induced immunosuppression, by significantly increasing (p < 0.05) the hemagglutinating antibody titre at dose of 200 mg/kg/p.o. and also significantly (p < 0.05) potentiated delayed type hypersensitivity reaction induced by sheep red blood cells. There was a significant (p < 0.05) increase in the phagocytic index, percentage neutrophil adhesion and significantly prevented the mortality induced by bovine Pasteurella multocida in mice when compared with control group. Result findings shows that both the extracts have ability to inhibit immunosuppression induced by cyclophosamide. Conclusion Study finding shows that AEAF and EEAF (at dose 200 mg/kg) extracts exerts immunostimulatory effects on specific and nonspecific immune system thus chemical constituents of herbal extracts may have applications as an effective immunotherapeutic agent and its potential application in the treatment of immune-related diseases.

In the present work simple fast, accurate, precise and robust method for analysis of pregabalin using derivatizing agent is reported. Method is simple as it requires no tedious sample preparation and hence can be used for routine analysis of pregabalin. The maximum absorbance of the pregabalin was found to be 210nm. The maximum UV absorption of the derivatized pregabalin was determined at 226nm. The given method was found to be linear in the concentration range 01–20 μg/mL. The performance of the develop method was evaluated in terms of standard deviation and relative standard deviation to find out the significance of proposed methods over the reference spectrophotometric method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also precise.