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Single-particle tracking (SPT) has become a popular tool to study the intracellular transport of molecules in living cells. Inferring the character of their dynamics is important, because it determines the organization and functions of the cells. For this reason, one of the first steps in the analysis of SPT data is the identification of the diffusion type of the observed particles. The most popular method to identify the class of a trajectory is based on the mean square displacement (MSD). However, due to its known limitations, several other approaches have been already proposed. With the recent advances in algorithms and the developments of modern hardware, the classification attempts rooted in machine learning (ML) are of particular interest. In this work, we adopt two ML ensemble algorithms, i.e. random forest and gradient boosting, to the problem of trajectory classification. We present a new set of features used to transform the raw trajectories data into input vectors required by the classifiers. The resulting models are then applied to real data for G protein-coupled receptors and G proteins. The classification results are compared to recent statistical methods going beyond MSD., 32 pages, 5 figures

In this paper, we have proposed a statistical procedure for detecting transitions of the mean-square-displacement exponent value within a single trajectory. With this procedure, we have identified three regimes of proteins dynamics on a cell membrane, namely, subdiffusion, free diffusion, and immobility. The fourth considered dynamics type, namely, superdiffusion was not detected. We show that the analyzed protein trajectories are not stationary and not ergodic. Moreover, classification of the dynamics type performed without prior detection of transitions may lead to the overestimation of the proportion of subdiffusive trajectories.

Taking advantage of recent single-particle tracking data, we compare the popular standard mean-squared displacement method with a statistical testing hypothesis procedure for three testing statistics and for two particle types: membrane receptors and the G proteins coupled to them. Each method results in different classifications. For this reason, more rigorous statistical tests are analyzed here in detail. The main conclusion is that the statistical testing approaches might provide good results even for short trajectories, but none of the proposed methods is ``the best'' for all considered examples; in other words, one needs to combine different approaches to get a reliable classification.

In this paper, we deal with a problem of local damage detection in bearings in the presence of a high-energy impulsive noise. Such a problem was identified during diagnostics of bearings in raw materials crusher. Unfortunately, classical approaches cannot be applied due to the impulsive character of the noise. In this paper we propose, a procedure that cancels out impulsive noise rather than extracts signal of interest. The methodology is based on the regime switching model with two regimes: first corresponding to high-energy noncyclic impulses and second to the rest of the signal. We apply the proposed technique to a simulated signal as well as to the real one. Effectiveness of the method is presented graphically using time series, time-frequency spectrogram, and classical envelope analysis. The obtained results indicate efficiency of the method in impulsive noise cancellation and improve the ability to detect a damage.

Solar flares are large explosions in the sun’s atmosphere. They can damage satellites and overload electrical systems. To manage that risk, finding methods of efficiently predicting future events is very important. In this paper we introduce a full-Sun flare prediction method based on the Hidden Markov modelling with two hidden states. We concentrate on the soft X-ray emission data near the minimum of solar cycle and consider two different driving dynamics for both states, namely the independent identically distributed (IID) random variables and the autoregressive (AR) processes, the latter introducing a memory structure. We compare prediction performance for the IID and AR approaches and also with a naive prediction equal to the last observation. The solar X-flux dynamics is predicted by using the day-ahead forecasts. We calculate point and interval forecasts and perform relevant statistical tests to choose the best method. It appears that the AR approach is clearly superior to the IID and naive both by means of point and interval forecasts. Moreover, it can well detect the higher state which can lead to very strong energy releases. Significant development of the model would be necessary to forecast solar flares over an entire solar cycle.

In this paper, we study ergodic properties of α-stable autoregressive fractionally integrated moving average (ARFIMA) processes which form a large class of anomalous diffusions. A crucial practical question is how long trajectories one needs to observe in an experiment in order to claim that the analyzed data are ergodic or not. This will be solved by checking the asymptotic convergence to 0 of the empirical estimator F(n) for the dynamical functional D(n) defined as a Fourier transform of the n-lag increments of the ARFIMA process. Moreover, we introduce more flexible concept of the e-ergodicity.

In this paper we study asymptotic behavior of a dynamical functional for an α-stable autoregressive fractionally integrated moving average (ARFIMA) process. We find an analytical formula for this important statistics and show its usefulness as a diagnostic tool for ergodic properties. The obtained results point to the very fast convergence of the dynamical functional and show that even for short trajectories one may obtain reliable conclusions on the ergodic properties of the ARFIMA process. Moreover we use the obtained theoretical results to illustrate how the dynamical functional statistics can be used in the verification of the proper model for an analysis of some biophysical experimental data.