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Fluorescence based microarray detection systems provide sensitive measurements; however, variation of probe immobilization and poor repeatability negatively affect the final readout, and thus quantification capability of these systems. Here, we demonstrate a label-free and high-throughput optical biosensor that can be utilized for calibration of fluorescence microarrays. The sensor employs a commercial flatbed scanner, and we demonstrate transformation of this low cost (similar to 100 USD) system into an Interferometric Reflectance Imaging Sensor through hardware and software modifications. Using this sensor, we report detection of DNA hybridization and DNA directed antibody immobilization on label-free microarrays with a noise floor of similar to 30 pg/mm(2), and a scan speed of 5 s (50 s for 10 frames averaged) for a 2 mm X 2 mm area. This novel system may be used as a standalone label-free sensor especially in low-resource settings, as well as for quality control and calibration of microarrays in existing fluorescence-based DNA and protein detection platforms.

The afterglow emission of some gamma-ray bursts (GRBs) show a shallow decay (plateau) phase implying continuous injection of energy. The source of this energy is very commonly attributed to the spin-down power of a nascent millisecond magnetar. The magnetic dipole radiation torque is considered to be the mechanism causing the spin-down of the neutron star. This torque has a component working for the alignment of the angle between rotation and magnetic axis, i.e., inclination angle, which has been neglected in modelling GRB afterglow light curves. Here, we demonstrate the evolution of the inclination angle and magnetic dipole moment of nascent magnetars associated with GRBs. We constrain the initial inclination angle, magnetic dipole moment and rotation period of seven magnetars by modelling the seven long-GRB afterglow light curves. We find that, in its first day, the inclination angle of a magnetar decreases rapidly. The rapid alignment of the magnetic and rotation axis may address the lack of persistent radio emission from mature magnetars. We also find that in three cases the magnetic dipole moments of magnetars decrease exponentially to a value a few times smaller than the initial value. The braking index of nascent magnetars, as a result of the alignment and magnetic dipole moment decline, is variable during the afterglow phase and always greater than three., 13 pages, 8 figures, 4 tables, accepted for publication in ApJ

In this paper, we demonstrate utilization of a commercial flatbed document scanner as a label-free biosensor for high-throughput imaging of DNA and protein microarrays. We implemented an interferometric sensing technique through use of a silicon/oxide layered substrate, and easy to implement hardware modifications such as re-aligning moving parts and inserting a custom made sample plate. With a cost as low as 100USD, powered by a USB cable, and scan speed of 30 seconds for a 4mm x 4 mm area with similar to 10 mu m lateral resolution, the presented system offers a super low cost, easy to use alternative to commercially available label-free systems., NA