1. Simultaneous biohydrogen (H2) and bioplastic (poly-β-hydroxybutyrate-PHB) productions under dark, photo, and subsequent dark and photo fermentation utilizing various wastes
- Author
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Arokiyaraj Selvaraj, K. Mohanrasu, Dai-Viet N. Vo, Ramu Satheesh Murugan, T. Angelin Swetha, G. Sivapraksh, Quang-Vu Bach, Hai Nguyen Tran, A. Arun, Balasubramani Ravindran, G.H. Dinesh, Soon Woong Chang, Dinh Duc Nguyen, and M. Jothi Basu
- Subjects
genetic structures ,biology ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Bacillus cereus ,food and beverages ,Energy Engineering and Power Technology ,02 engineering and technology ,Dark fermentation ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,biology.organism_classification ,01 natural sciences ,Bioplastic ,Husk ,Hydrolysate ,0104 chemical sciences ,Fuel Technology ,Wastewater ,Fermentation ,Biohydrogen ,Food science ,0210 nano-technology - Abstract
The present study is focused on bio hydrogen (H2) and bioplastic (i.e., poly-β-hydroxybutyrate; PHB) productions utilizing various wastes under dark fermentation, photo fermentation and subsequent dark-photo fermentation. Potential bio H2 and PHB producing microbes were enriched and isolated. The effects of substrate (rice husk hydrolysate, rice straw hydrolysate, dairy industry wastewater, and rice mill wastewater) concentration (10–100%) and pH (5.5–8.0) were examined in the batch mode under the dark and photo fermentation conditions. Using 100% rice straw hydrolysate at pH 7, the maximum bio H2 (1.53 ± 0.04 mol H2/mol glucose) and PHB (9.8 ± 0.14 g/L) were produced under dark fermentation condition by Bacillus cereus. In the subsequent dark-photo fermentation, the highest amounts of bio H2 and PHB were recorded utilizing 100% rice straw hydrolysate (1.82 ± 0.01 mol H2/mol glucose and 19.15 ± 0.25 g/L PHB) at a pH of 7.0 using Bacillus cereus (KR809374) and Rhodopseudomonas rutila. The subsequent dark-photo fermentative bio H2 and PHB productions obtained using renewable biomass (i.e., rice husk hydrolysate and rice straw hydrolysate) can be considered with respect to the sustainable management of global energy sources and environmental issues.
- Published
- 2020