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Low-cost, paper-based platforms are gaining increasing relevance in modern diagnostics and biosensing. Most diagnostic systems using blood samples require blood-plasma separation to reduce complexity and achieve better results. Blood plasma separation is often carried-out by using various membrane based filters. This paper presents a new method for capturing red blood cells on Whatman filter paper with no. 1, 2, 5 and glass fiber discs. All four paper types were subjected to modification with hydrothermally grown zinc oxide nanorods. Growth and morphologies of zinc oxide nanorods were confirmed by SEM, XRD and XPS studies. The blood plasma separation experiments were done by cutting the papers into identical strips with dimension of 5 × 20 mm2. 3 μL of blood was mixed with equal volume of antibody solution and pipetted on each strip. The colored antibody solution was used to track the path of antibody, eventually the separated plasma. The dried strips were scanned and the difference in the distance travelled by colored solution of antibodies was measured. From the results, it is clear that all four paper strips covered with nanorods have shown more distance travelled and better separation than their control samples.

Emotion recognition gains huge popularity now a days. Physiological signals provides an appropriate way to detect human emotion with the help of IoT. In this paper, a novel system is proposed which is capable of determining the emotional status using physiological parameters, including design specification and software implementation of the system. This system may have a vivid use in medicine (especially for emotionally challenged people), smart home etc. Various Physiological parameters to be measured includes, heart rate (HR), galvanic skin response (GSR), skin temperature etc. To construct the proposed system the measured physiological parameters were feed to the neural networks which further classify the data in various emotional states, mainly in anger, happy, sad, joy. This work recognized the correlation between human emotions and change in physiological parameters with respect to their emotion.

Background Periparturient period induces stress in cows which fluctuates hormonal and metabolic function and causes immune suppression. Apart from impairing the health, production, and reproduction of cows, it also influences the well-being of newborn calves by decreasing the colostrum quality. Micronutrients are known for optimal health and production and their effects on parturition stress, immune response in both cow and its calf need to be explored. Aim The aim of this study was to see the effect of oral supplementation of micronutrients during the prepartum period on the health status of crossbred dairy cows and subsequently on their newborn calves. Methods A total of 42 healthy multiparous cows were selected and randomly divided into five groups with seven cows in each group, i.e. control (Basal Diet, BD), VA group (BD + vitamin A, 105 IU), Zn group (BD + zinc sulphate, 60 ppm), VE group (BD + vitamin E, 2500 IU), and combined supplementation (CS) group (BD + combination of VA, Zn, and VE). The supplements were offered in compounded concentrate DM (100 g) to individual cows once daily before the morning feeding and the remaining portion was incorporated in the TMR. Feeding was started one month before the expected days of calving till calving. Blood samples were collected from cows at days -15, -7, -3, 0, +3, +7, and +15 relative to the day of calving. Blood samples from newborn calves and milk samples of cows were collected at days 0, +3, +7, and +15. Milk somatic cell counts (SCC) were estimated using a cell counter. Cortisol was estimated by ELISA kit in blood and milk plasma of cows and in the blood plasma of their calves. Total immunoglobulins (Ig) were estimated in milk of cows and serum of calves using zinc sulphate turbidity method. Blood neutrophils from cows and calves were studied for phagocytic activity (PA) using nitro blue tetrazolium (NBT) assay.Data were analysed by repeated-measures two-way ANOVA using the mixed procedure of SAS, and the pairwise comparison was performed using a multiple comparison test (Tukey). Results Combined supplementation of micronutrients decreased (P Conclusion Our results indicate that micronutrient interventions during the prepartum period can improve the health status of dairy calves and subsequently the well-being of their calves.

Distinguishing a specific biomarker from a biofluid sample containing a large variety of proteins often requires the selective preconcentration of that particular biomarker to a detectable level for analysis. Low-cost, paper-based device is an emerging opportunity in diagnostics. In the present study, we report a novel Zinc oxide nanorods functionalized paper platform for the preconcentration of Myoglobin, a cardiac biomarker. Zinc oxide nanorods were grown on a Whatman filter paper no. 1 via the standard hydrothermal route. The growth of Zinc oxide nanorods on paper was confirmed by a combination of techniques consisting of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS,) scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDX) analysis. The Zinc oxide nanorods modified Whatman filter paper (ZnO-NRs/WFP) was further tested for use as a protein preconcentrator. Paper-based ELISA was performed for determination of pre-concentration of cardiac marker protein Myoglobin using the new ZnO-NRs/WFP platform. The ZnO-NRs/WFP could efficiently capture the biomarker even from a very dilute solution (Myoglobin