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Your search keyword '"MESH: Hordeum"' showing total 2 results
Start Over Descriptor "MESH: Hordeum" Topic 01 natural sciences
2 results on '"MESH: Hordeum"'

1. Hordeins are expressed in microspore-derived embryos and also during male gametophytic and very early stages of seed development

Author

Amada Pulido, Adela Olmedilla, Beáta Barnabás, Alberto Hernando, Martine Devic, Ferenc Bakos, Enrique Méndez, Laboratoire Génome et développement des plantes (LGDP), and Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Glutens, Physiology, Blotting, Western, Stamen, Enzyme-Linked Immunosorbent Assay, Plant Science, Biology, 01 natural sciences, Endosperm, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, Tissue Culture Techniques, 03 medical and health sciences, MESH: In Situ Hybridization, Microspore, MESH: Reverse Transcriptase Polymerase Chain Reaction, Botany, MESH: Blotting, Western, MESH: Tissue Culture Techniques, RNA, Messenger, In Situ Hybridization, MESH: RNA, Messenger, Plant Proteins, 030304 developmental biology, 0303 health sciences, MESH: Plant Proteins, Reverse Transcriptase Polymerase Chain Reaction, Embryogenesis, MESH: Enzyme-Linked Immunosorbent Assay, food and beverages, MESH: Immunohistochemistry, Hordeum, Embryo, Immunohistochemistry, Cell biology, MESH: Seeds, Protein body, Hordein, MESH: Hordeum, MESH: Pollen, Seeds, Pollen, Hordeum vulgare, Prolamins, 010606 plant biology & botany
Abstract

Microspore-derived embryos induced by anther or isolated-microspore culture display certain characteristics of zygotic embryos. Furthermore, the expression of certain endosperm genes has been described in these non-zygotic embryos. The expression of hordein genes encoding the main barley endosperm proteins has been studied using a wide range of methods (RT-PCR, in situ hybridization, ELISA sandwich, western blotting immunocytochemistry, and cytochemistry) to ascertain their presence or absence during the induction and first stages of microspore embryogenesis. Due to the very sensitive techniques used it was possible to detect for the first time hordein expression during microspore embryogenesis. Surprisingly, these hordeins were also detected at different stages of male gametophytic development as well as during the very early stages of seed development, when they have not hitherto been detected. The expression and localization of these storage proteins and their corresponding transcripts provide new information about barley microspore embryogenesis and its relationship to zygotic embryogenesis. Although only small quantities of hordeins are accumulated during microspore embryogenesis they seem to be necessary for the initial development of the microspore-derived embryo. This idea is supported by the changes detected in their concentration throughout this process and is in accordance with previously published data concerning the importance of endosperm proteins for embryo development in both microspore culture and in planta.

Published
2006
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2. Diversity and evolution of the Hordeum murinum polyploid complex in Algeria

Author

Malika L. Aïnouche, Spencer Brown, Abdelkader Aïnouche, Olivier Coriton, Rachid Amirouche, Marie-Thérèse Misset, Malika Ourari, Virginie Huteau, Faculté des Lettres et des Sciences humaines - Université de Béjaïa (FLSH), Université Abderrahmane Mira [Béjaïa], Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Amélioration des Plantes et Biotechnologies Végétales (APBV), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB), Programme Hubert Curien CMEP-Tassili 08 MDU 724 'Polyploidy, Genome evolution and Biodiversity', Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université des Sciences et de la Technologie Houari Boumediene = University of Sciences and Technology Houari Boumediene [Alger] (USTHB), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, MESH: Genome, Plant, Climate, MESH: Flow Cytometry, Evolution moléculaire, MESH: Base Sequence, 01 natural sciences, Genome, MESH: Ploidies, MESH: Chromosomes, Plant, MESH: Genetic Variation, MESH: Phylogeny, In Situ Hybridization, Phylogeny, 0303 health sciences, MESH: DNA, Ribosomal, biology, MESH: Genomics, RNA, Ribosomal, 5S, food and beverages, General Medicine, Polyploid complex, Genomics, Hordeum murinum L, MESH: Climate, Flow Cytometry, Biological Evolution, Meiosis, Phylogeography, MESH: Phylogeography, MESH: Hordeum, Ploidy, MESH: Algeria, Genome, Plant, Biotechnology, DNA, Plant, Mitosis, MESH: Biological Evolution, DNA, Ribosomal, Chromosomes, Plant, 03 medical and health sciences, Cytogenetics, MESH: In Situ Hybridization, Molecular evolution, Hordeum murinum, Botany, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, MESH: RNA, Ribosomal, 5S, POLYPLOIDIE, MESH: DNA, Plant, Molecular Biology, Gene, MESH: Cytogenetics, 030304 developmental biology, Ploidies, Base Sequence, fungi, Genetic Variation, Hordeum, MESH: Mitosis, biology.organism_classification, MESH: Meiosis, Taxon, RNA, Ribosomal, Algeria, FISH–GISH, MESH: RNA, Ribosomal, 010606 plant biology & botany
Abstract

International audience; Population diversity and evolutionary relationships in the Hordeum murinum L. polyploid complex were explored in contrasted bioclimatic conditions from Algeria. A multidisciplinary approach based on morphological, cytogenetic, and molecular data was conducted on a large population sampling. Distribution of diploids (subsp. glaucum) and tetraploids (subsp. leporinum) revealed a strong correlation with a North-South aridity gradient. Most cytotypes exhibit regular meiosis with variable irregularities in some tetraploid populations. Morphological analyses indicate no differentiation among taxa but high variability correlated with bioclimatic parameters. Two and three different nuclear sequences (gene coding for an unspliced genomic protein kinase domain) were isolated in tetraploid and hexaploid cytotypes, respectively, among which one was identical with that found in the diploid subsp. glaucum. The tetraploids (subsp. leporinum and subsp. murinum) do not exhibit additivity for 5S and 45S rDNA loci comparative with the number observed in the related diploid (subsp. glaucum). The subgenomes in the tetraploid taxa could not be differentiated using genomic in situ hybridization (GISH). Results support an allotetraploid origin for subsp. leporinum and subsp. murinum that derives from the diploid subsp. glaucum and another unidentified diploid parent. The hexaploid (subsp. leporinum) has an allohexaploid origin involving the two genomes present in the allotetraploids and another unidentified third diploid progenitor.

Published
2011
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